1、January 2012 Translation by DIN-Sprachendienst.English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).IC
2、S 65.080!$y+B“1860831www.din.deDDIN EN 16086-1Soil improvers and growing media Determination of plant response Part 1: Pot growth test with Chinese cabbageEnglish translation of DIN EN 16086-1:2012-01Bodenverbesserungsmittel und Kultursubstrate Bestimmung der Pflanzenvertrglichkeit Teil 1: Wachstums
3、test mit Chinakohl im TopfEnglische bersetzung von DIN EN 16086-1:2012-01Amendements du sol et supports de culture Dtermination de la rponse des plantes Partie 1: Essai de croissance en pot avec du chou de ChineTraduction anglaise de DIN EN 16086-1:2012-01www.beuth.deDocument comprises pagesIn case
4、of doubt, the German-language original shall be considered authoritative.2701.12 DIN EN 16086-1:2012-01 2 A comma is used as the decimal marker. National foreword This standard has been prepared by Technical Committee CEN/TC 223 “Soil improvers and growing media” (Secretariat: ASI, Austria). The res
5、ponsible German body involved in its preparation was the Normenausschuss Lebensmittel und landwirtschaftliche Produkte (Food and Agricultural Products Standards Committee), Working Committee NA 057-03-01 AA Bodenverbesserungsmittel und Kultursubstrate. The DIN Standards corresponding to the Internat
6、ional Standards referred to in this document are as follows: ISO 22030 DIN EN ISO 22030 EN ISO 3696 DIN ISO 3696 DIN EN 16086 consists of the following parts, under the general title Soil improvers and growing media Determination of plant response: Part 1: Pot growth test with Chinese cabbage Part 2
7、: Petri dish test using cress National Annex NA (informative) Bibliography DIN ISO 3696, Water for analytical laboratory use Specification and test methods DIN EN ISO 22030, Soil quality Biological methods Chronic toxicity in higher plants EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 16086-1
8、 November 2011 ICS 65.080 English Version Soil improvers and growing media - Determination of plant response - Part 1: Pot growth test with Chinese cabbage Amendements du sol et supports de culture - Dtermination de la rponse des plantes - Partie 1: Essai de croissance en pot avec du chou de Chine B
9、odenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenvertrglichkeit - Teil 1: Wachstumstest mit Chinakohl im Topf This European Standard was approved by CEN on 17 September 2011. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions
10、for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in th
11、ree official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards b
12、odies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
13、Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16086-1:2011: E
14、EN 16086-1:2011 (E) 2 Contents Page Foreword 41 Scope 52 Normative references 53 Terms and definitions .54 Principle of the plant growth response test 64.1 General 64.2 Pot experiment with direct use of the prepared sample 64.3 Pot experiment using an extract of the original sample 65 Choice of meth
15、odology .66 Pot experiment with direct use of the prepared sample 66.1 Materials .66.1.1 Water of class 366.1.2 Sphagnum peat 66.1.3 Fertilized and limed sphagnum peat66.1.4 Ground limestone 76.1.5 Seeds of Chinese cabbage (Brassica napa, ssp. pekinensis) 76.1.6 Seeds of Spring barley (Hordeum vulga
16、re) .76.2 Apparatus .76.3 Preparation of the sample .76.3.1 General preparation .76.3.2 Mixing procedure .86.4 Test procedure 106.5 Control sample 106.6 Validity of the test . 106.7 Evaluation parameters . 116.7.1 Germination rate (%) 116.7.2 Fresh weight 126.7.3 Growth inhibition 136.7.4 Abnormalit
17、ies in comparison to the control 137 Pot experiment using an extract of the original sample . 147.1 Materials 147.1.1 Water of class 3. 147.1.2 Perlite . 147.1.3 Seeds of Chinese cabbage (Brassica napa, ssp. pekinensis) . 147.1.4 Seeds of Spring barley (Hordeum vulgare) 147.1.5 Balanced nutrient sol
18、ution (see B.1) 147.1.6 Nitric acid (HNO3), 1M . 147.2 Apparatus 147.2.1 Sieve with 20 mm mesh size 147.2.2 Circular plant pot 147.2.3 Saucer 147.2.4 Bucket 147.2.5 Thin fleece or plastic sheet for covering the containers 157.2.6 Testing facility . 157.2.7 Irrigation device for watering the pots . 1
19、57.2.8 Analytical balance. 157.3 Preparation of the sample 157.3.1 General preparation 15DIN EN 16086-1:2012-01 EN 16086-1:2011 (E) 3 7.3.2 Extraction procedure 157.4 Test procedure . 157.5 Control sample 167.6 Validity of the test 167.7 Evaluation parameters 168 Precision. 169 Test report 16Annex A
20、 (informative) Validation . 18Annex B (normative) Nutrient supply and fist test 21B.1 Composition of the nutrient solution 21B.2 Possible adjustments of the nutrient supply during the test . 22B.2.1 Possible reasons for nutritional adjustments 22B.2.2 Recommendations for nutritional adjustments . 22
21、B.2.3 Supplement to the report 23B.3 Fist test . 24Bibliography 25DIN EN 16086-1:2012-01 EN 16086-1:2011 (E) 4 Foreword This document (EN 16086-1:2011) has been prepared by Technical Committee CEN/TC 223 “Soil improvers and growing media”, the secretariat of which is held by ASI. This European Stand
22、ard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2012, and conflicting national standards shall be withdrawn at the latest by May 2012. Attention is drawn to the possibility that some of the elements of this docu
23、ment may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. SAFETY PRECAUTIONS Care should be taken when handling samples that may contain sharps or is of a dusty nature. According to the CEN/CENELEC Internal Regulations,
24、the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Neth
25、erlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. DIN EN 16086-1:2012-01 EN 16086-1:2011 (E) 5 1 Scope This European Standard describes a method for the routine determination of the effect of soil improvers and growing media or constit
26、uents thereof on the growth of Chinese cabbage (and in certain cases spring barley). This test may not be suitable for all growing media since the growing media characteristics (e.g. nutrient content) will vary according to target use and the product is not tested in accordance with the specified us
27、e and pack recommendations. This test is not appropriate for the detection of nitrogen immobilization. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the l
28、atest edition of the referenced document (including any amendments) applies. EN 13037, Soil improvers and growing media Determination of pH EN 13038, Soil improvers and growing media Determination of electrical conductivity EN 13040, Soil improvers and growing media Sample preparation for chemical a
29、nd physical tests, determination of dry matter content, moisture content and laboratory compacted bulk density EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696:1987) 3 Terms and definitions For the purposes of this document, the following terms and definition
30、s apply. 3.1 plant response variation in plant germination and/or growth when sown and grown in a growing medium, soil improver or constituent thereof or in an extract obtained from these materials Factors causing negative plant growth cannot be identified nor quantified by applying this method. 3.2
31、 prepared sample portion of the laboratory sample, undiluted or diluted with sphagnum peat at given ratios, fertilized and limed as required DIN EN 16086-1:2012-01 EN 16086-1:2011 (E) 6 4 Principle of the plant growth response test 4.1 General Depending on the material to be tested, one of the two m
32、ethods described in this standard shall be used. 4.2 Pot experiment with direct use of the prepared sample Sowing a defined quantity of Chinese cabbage into pots containing the prepared sample, cultivating under controlled conditions for a defined period of time and evaluating the plant response by
33、determining the germination rate, fresh weight, abnormalities and overall plant growth. If the presence of graminacious herbicides is suspected, Spring barley shall be used in addition to Chinese cabbage. For testing of other specific effects, the use of additional plant species (for example lettuce
34、) can be considered. 4.3 Pot experiment using an extract of the original sample Mixing the original sample with nutrient solution as an extractant, soaking for 4 h at ambient temperature and collecting the freely available nutrient solution. Filling pots with perlite saturated with the extract and c
35、ontinuing as described under 4.2, irrigating during the test period with a fixed quantity of the extract and afterwards water. If the presence of graminacious herbicides is suspected, Spring barley shall be used in addition to Chinese cabbage. For testing of other specific effects, the use of additi
36、onal plant species (for example lettuce) can be considered. 5 Choice of methodology For most materials, the pot growth test can be carried out as described in Clause 6. However, for coarse materials such as bark, expanded clay, lava, mineral wool, perlite, polyurethane and pumice with an inherently
37、low water holding capacity which are used as growing media without amendment, this procedure is unsuitable and the extract method described in Clause 7 shall be adopted. 6 Pot experiment with direct use of the prepared sample 6.1 Materials 6.1.1 Water of class 3 According to EN ISO 3696. 6.1.2 Sphag
38、num peat Sphagnum peat with a degree of humification of H3 H5 according to von Post scale, having a pH measured according to EN 13037 of between 3,0 and 4,5, an EC measured according to EN 13038 of between 1 mS m-1and 5 mS m-1, a particle size of 10 mm and to which neither lime nor fertilizer has be
39、en added. 6.1.3 Fertilized and limed sphagnum peat Sphagnum peat (see 6.1.2), having a pH measured according to EN 13037 adjusted using ground limestone (see 6.1.4) to a range between 5,5 and 6,5, fertilized with a water soluble “complete” fertilizer with essential DIN EN 16086-1:2012-01 EN 16086-1:
40、2011 (E) 7 micronutrients, added at a rate to supply (225 25) mg N l-1, (for example 1,5 g l-1water soluble complete fertilizer N : P2O5 : K2O 15 : 10 : 20), (see B.1). 6.1.4 Ground limestone Finely ground limestone, containing at least 5 % MgCO3, having a particle size less than 1 mm and a moisture
41、 content of less than 1 % m/m. 6.1.5 Seeds of Chinese cabbage (Brassica napa, ssp. pekinensis) Specified germination capacity 95 %. 6.1.6 Seeds of Spring barley (Hordeum vulgare) Specified germination capacity 95 %. 6.2 Apparatus 6.2.1 Sieve with 20 mm mesh size 6.2.2 Sieve with 5 mm mesh size 6.2.3
42、 Circular plant pot Upper diameter (12 0,5) cm , height between 8,5 cm and 9,0 cm, volume between 650 ml and 700 ml, perforated bottom to provide drainage (for example plastic pot used in horticulture). 6.2.4 Saucer Saucer capable of catching all surplus water from the plant container after overhead
43、 watering. 6.2.5 Thin fleece or plastic sheet for covering the containers 6.2.6 Testing facility Testing facility capable of maintaining and monitoring the temperature and light intensity specified in 6.4 such as a greenhouse or plant growth room. 6.2.7 Irrigation device for watering the pots For ex
44、ample watering can, greenhouse watering hose 6.2.8 Analytical balance Accuracy 0,01 g, capacity 500 g. 6.3 Preparation of the sample 6.3.1 General preparation Pass the sample through a 20 mm sieve (see 6.2.1). Any foreign material such as plastic, metal or glass retained on the sieve shall be remove
45、d and noted. Other material that is retained on the sieve and which is an intrinsic part of the sample shall be physically reduced to parts of similar size as few times as are necessary to permit the entire sample to pass through the sieve. Fibrous materials i.e. coir fibres and straw shall be cut t
46、o a length 20 mm by using scissors. Thoroughly mix the laboratory sample with the broken particles that had been retained on the sieve taking care to minimise physical damage to the sample as a whole. Transportation DIN EN 16086-1:2012-01 EN 16086-1:2011 (E) 8 and possible storage of the samples sha
47、ll be done in accordance with EN 13040, using food grade polyethylene bags. 6.3.2 Mixing procedure Before mixing, the laboratory compacted bulk density shall be measured (according to EN 13040). To produce the prepared sample, the material prepared as described in 6.3.1 is thoroughly mixed with spha
48、gnum peat (see 6.1.2) on a volume/volume basis as given in Table 1. The pH according to EN 13037 is ideally within the range between 5,5 and 6,5. If it is below, the pH shall be adjusted by adding limestone (see 6.1.4). If it is above, the pH shall be noted. Additionally, a water soluble complete fertilizer with essential micronutrients, added at a rate to supply (225 25) mg N l
