1、July 2014 Translation by DIN-Sprachendienst.English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 1
2、3.080.30!%20“2159313www.din.deDDIN EN ISO 11267Soil quality Inhibition of reproduction of Collembola (Folsomia candida) by soilcontaminants (ISO 11267:2014);English version EN ISO 11267:2014,English translation of DIN EN ISO 11267:2014-07Bodenbeschaffenheit Hemmung der Reproduktion von Collembolen (
3、Folsomia candida) durch Verunreinigungen(ISO 11267:2014);Englische Fassung EN ISO 11267:2014,Englische bersetzung von DIN EN ISO 11267:2014-07Qualit du sol Inhibition de la reproduction de Collembola (Folsomia candida) par des contaminants dusol (ISO 11267:2014);Version anglaise EN ISO 11267:2014,Tr
4、aduction anglaise de DIN EN ISO 11267:2014-07SupersedesDIN ISO 11267:2001-06www.beuth.deIn case of doubt, the German-language original shall be considered authoritative.Document comprises 27 pages06.14 DIN EN ISO 11267:2014-07 2 A comma is used as the decimal marker. National foreword This document
5、(EN ISO 11267:2014) has been prepared by Technical Committee ISO/TC 190 “Soil quality” in collaboration with Technical Committee CEN/TC 345 “Characterization of soils” (Secretariat: NEN, Netherlands). The preliminary work was done by Working Group WG 2 “Effects on soil fauna” of ISO/TC 190/SC 4 (lea
6、d-management: DIN, Germany). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-02-04 UA Biologische Verfahren of NA 119-01-02 AA Abfall- und Bodenuntersuchung. The DIN Standards corresponding
7、to the International Standards referred to in this document are as follows: ISO 10381-6 DIN ISO 10381-6 ISO 10390 DIN ISO 10390 ISO 10694 DIN ISO 10694 ISO 11260 DIN EN ISO 11260 ISO 11268-2 DIN ISO 11268-2 ISO 11277 DIN ISO 11277 ISO 11465 DIN ISO 11465 ISO 15799 DIN ISO 15799 ISO 16387 DIN EN ISO
8、16387 ISO 17616 DIN ISO 17616 Amendments This standard differs from DIN ISO 11267:2001-06 as follows: a) Clause 10 “Statistical analysis” has been supplemented; b) the standard has been editorially revised. Previous editions DIN ISO 11267: 2001-06 DIN EN ISO 11267:2014-07 3 National Annex NA (inform
9、ative) Bibliography DIN EN ISO 11260, Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution DIN EN ISO 16387, Soil quality Effects of contaminants on Enchytraeidae (Enchytraeus sp.) Determina-tion of effects on reproduction DIN ISO
10、10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory DIN ISO 10390, Soil quality Determination of pH DIN ISO 10694, Soil quality Determination
11、of organic and total carbon after dry combustion (elementary analysis) DIN ISO 11268-2, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 2: Determination of effects on reproduction DIN ISO 11277, Soil quality Determination of particle size distribution in mineral soil material
12、Method by sieving and sedimentation DIN ISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravimetric method DIN ISO 15799, Soil quality Guidance on the ecotoxicological characterization of soils and soil materials DIN ISO 17616, Soil quality Guidance on the choic
13、e and evaluation of bioassays for ecotoxicological characterization of soils and soil materials DIN EN ISO 11267:2014-07 4 This page is intentionally blank EN ISO 11267February 2014 ICS 13.080.30 English VersionSoil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contam
14、inants (ISO 11267:2014)Qualit du sol - Inhibition de la reproduction de Collembola (Folsomia candida) par des contaminants du sol (ISO 11267:2014) Bodenbeschaffenheit - Hemmung der Reproduktion von Collembolen (Folsomia candida) durch Verunreinigungen (ISO 11267:2014) This European Standard was appr
15、oved by CEN on 4 January 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
16、 standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language
17、and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hun
18、gary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any
19、form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11267:2014 EEUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGEUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMContents Page Foreword . 3 Introduction . 4 1 Scope . 5 2 No
20、rmative references. 5 3 Terms and definitions 5 4 Principle 7 5 Reagents and material . 8 6 Apparatus 10 7 Procedure 10 7.1 Experimental design 10 7.2 Preparation of test mixture . 11 7.3 Addition of the biological material . 13 7.4 Test conditions and measurements . 13 7.5 Determination of survivin
21、g Collembola . 13 8 Calculation and expression of results . 13 8.1 Calculation 13 8.2 Expression of results . 13 9 Validity of the test 14 10 Statistical analysis . 14 10.1 General 14 10.2 Single-concentration tests 14 10.3 Multi-concentration tests 15 11 Test report . 15 Annex A (informative) Techn
22、iques for rearing and breeding of Collembola . 17 Annex B (informative) Determination of water-holding capacity 19 Annex C (informative) Guidance on adjustment of pH of artificial soil 20 Annex D (informative) Extraction and counting of Collembola . 21 Bibliography 22 2DIN EN ISO 11267:2014-07EN ISO
23、 11267:2014 (E) Foreword This document (EN ISO 11267:2014) has been prepared by Technical Committee ISO/TC 190 “Soil quality” in collaboration with Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. This European Standard shall be given the status of
24、a national standard, either by publication of an identical text or by endorsement, at the latest by August 2014, and conflicting national standards shall be withdrawn at the latest by August 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of
25、patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
26、 Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey
27、and the United Kingdom. Endorsement notice The text of ISO 11267:2014 has been approved by CEN as EN ISO 11267:2014 without any modification. 3DIN EN ISO 11267:2014-07EN ISO 11267:2014 (E)IntroductionEcotoxicological test systems are applied to obtain information about the effects of contaminants in
28、 soil and are proposed to complement conventional chemical analysis (see 2 and 4). Reference 2 includes a list and short characterization of recommended and standardized test systems and 4 gives guidance on the choice and evaluation of the bioassays. Aquatic test systems with soil eluate are applied
29、 to obtain information about the fraction of contaminants potentially reaching the groundwater by the water path (retention function of soils), whereas terrestrial test systems are used to assess the habitat function of soils.Soil-dwelling Collembola are ecologically relevant species for ecotoxicolo
30、gical testing. Springtails are prey animals for a variety of endogeic and epigeic invertebrates and they contribute to decomposition processes in soils. In acidic soils they may be the most important soil invertebrates besides enchytraeids with respect to that function, since earthworms are typicall
31、y absent.19Additionally, Collembola represent arthropod species with a different route and a different rate of exposure compared to earthworms1and enchytraeids.3Various species were used in bioassays of which four species were used most commonly, Folsomia candida, Folsomia fimetaria, Onychiurus arma
32、tus, and Orchesella cincta.20Numerous soil toxicity tests supported by Environment Canada (EC) resulted in the development and standardization of a biological test method for determining the lethal and sublethal toxicity of samples of contaminated soil to Collembola.10The method prepared by EC inclu
33、des three species, Orthonychiurus folsomi, Folsomia candida, and Folsomia fimetaria. As standardized test systems using Collembola as indicator organisms for the habitat function of soil, another two methods exist. One is designed for assessing the effects of substances on the reproductive output of
34、 the Collembola, Folsomia fimetaria L. and Folsomia candida Willem in soil,19,21, and the other method described here, focuses on testing contaminated soil. Optionally the method can be used for testing substances added to standard soils (e.g. artificial soil) for their sublethal hazard potential to
35、 Collembola.This International Standard describes a method that is based on the determination of sublethal effects of contaminated soils to adult Collembola of the species Folsomia candida Willem. The species is distributed worldwide. It plays a similar ecological role to Folsomia fimetaria.10,19Fol
36、somia candida reproduces parthenogenetically and is an easily accessible species as it is commercially available and easy to culture. Folsomia candida is considered to be a representative of soil arthropods and Collembola in particular. Background information on the ecology of springtails and their
37、use in ecotoxicological testing is available.224DIN EN ISO 11267:2014-07EN ISO 11267:2014 (E) 1 ScopeThis International Standard specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candi
38、da Willem by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials.Effects of substances are assessed u
39、sing a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an un
40、contaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil).This International Standard provides information on how to use this method for testing substances under temperate conditions.The method is not applicable to volatile substances, i.e. subs
41、tances for which H (Henrys constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 C.NOTE The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persi
42、stence of the substance under test.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the refere
43、nced document (including any amendments) applies.ISO 10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratoryISO 10694, Soil quality Determination
44、of organic and total carbon after dry combustion (elementary analysis)ISO 10390, Soil quality Determination of pHISO 11260, Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solutionISO 11277, Soil quality Determination of particle size
45、distribution in mineral soil material Method by sieving and sedimentationISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravimetric method3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.5DIN EN ISO 11267:2014-
46、07EN ISO 11267:2014 (E)3.1contaminantsubstance or agent present in the soil as a result of human activity3.2ECxeffect concentration for x % effectconcentration (mass fraction) of a test substance that causes x % of an effect on a given end-point within a given exposure period when compared with a co
47、ntrolEXAMPLE An EC50 is a concentration estimated to cause an effect on a test end-point in 50 % of an exposed population over a defined exposure period.Note 1 to entry: The ECx is expressed as a percentage of soil to be tested (dry mass) per soil mixture (dry mass). When substances are tested, the
48、ECx is expressed as mass of the test substance per dry mass of soil in milligrams per kilogram.3.3ERxeffect raterate of a soil to be tested that causes an x % of an effect on a given end-point within a given exposure period when compared with a control3.4limit testsingle concentration test consistin
49、g of at least four replicates each, the soil to be tested without any dilution or the highest concentration of test substance mixed into the control soil and the control3.5LOEClowest observed effect concentrationlowest test substance concentration that has a statistically significant effect (probability p 2) or ionizing substances into account, 5 % of peat have proven to be sufficient for maintaining the desired
