1、September 2017 English price group 17No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 07.100.30!%mPf“2744567www.din.
2、deDIN EN ISO 11290-2Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 2: Enumeration method (ISO 112902:2017);English version EN ISO 112902:2017,English translation of DIN EN ISO 11290-2:2017-09Mikrobiologie der Leb
3、ensmittelkette Horizontales Verfahren fr den Nachweis und die Zhlung von Listeria monocytogenes und von Listeria spp. Teil 2: Zhlverfahren (ISO 112902:2017);Englische Fassung EN ISO 112902:2017,Englische bersetzung von DIN EN ISO 11290-2:2017-09Microbiologie de la chane alimentaire Mthode horizontal
4、e pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. Partie 2: Mthode de dnombrement (ISO 112902:2017);Version anglaise EN ISO 112902:2017,Traduction anglaise de DIN EN ISO 11290-2:2017-09SupersedesDIN EN ISO 112902:200501www.beuth.deDocument comprises 38 pagesDTransla
5、tion by DIN-Sprachendienst.In case of doubt, the German-language original shall be considered authoritative.08.17 DIN EN ISO 11290-2:2017-09 2 A comma is used as the decimal marker. National foreword This document (EN ISO 11290-2:2017) has been prepared by Technical Committee ISO/TC 34 “Food product
6、s” (Secretariat: AFNOR, France) in collaboration with Technical Committee CEN/TC 275 “Food analysis Horizontal methods” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was DIN-Normenausschuss Lebensmittel und landwirtschaftliche Produkte (DIN Standards Committee
7、Food and Agricultural Products), Working Committee NA 057-01-06 AA “Microbiology of the food chain”. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 6887 (all parts) DIN EN ISO 6887 (all parts) ISO 7218 DIN EN ISO 7218 ISO 11133 DIN EN
8、ISO 11133 ISO 11290-1 DIN EN ISO 11290-1 ISO 18593 DIN ISO 18593 ISO 5725-2:1994 DIN ISO 5725-2:2002-12 ISO 17468 DIN EN ISO 17468 ISO/TS 17728 DIN CEN ISO/TS 17728 (DIN SPEC 10053) Amendments This standard differs from DIN EN ISO 11290-2:2005-01 as follows: a) the enumeration of Listeria monocytoge
9、nes has been modified as listed below; b) primary suspension with buffered peptone water, half-Fraser broth with or without supplements, and all appropriate diluents referred to in ISO 6887 (all parts) has been included; c) the resuscitation step has been deleted; d) the microscopic aspect, catalase
10、 and CAMP test for confirmation have been made optional; e) new performance characteristics have been included; f) moreover, the enumeration of Listeria monocytogenes has been included in the scope and the title has been changed accordingly. Previous editions DIN EN ISO 11290-2: 1998-08, 2005-01 DIN
11、 EN ISO 11290-2:2017-09 3 National Annex NA (informative) Bibliography DIN CEN ISO/TS 17728 (DIN SPEC 10053), Microbiology of the food chain Sampling techniques for microbiological analysis of food and feed samples DIN EN ISO 6887 (all parts), Microbiology of the food chain Preparation of test sampl
12、es, initial suspension and decimal dilutions for microbiological examination DIN EN ISO 7218, Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations DIN EN ISO 11133, Microbiology of food, animal feed and water Preparation, production, stora
13、ge and performance testing of culture media DIN EN ISO 11290-1, Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection method DIN EN ISO 17468, Microbiology of the food chain Technical requirements and guida
14、nce on establishment or revision of a standardized reference method DIN ISO 5725-2:2002-12, Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method (ISO 5725-2:1994 including
15、 Technical Corrigendum 1:2002) DIN ISO 18593, Microbiology of food and animal feeding stuffs Horizontal methods for sampling techniques from surfaces using contact plates and swabs DIN EN ISO 11290-2:2017-09 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO
16、11290-2 June 2017 ICS 07.100.30 Supersedes EN ISO 11290-2:1998English Version Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method(ISO 11290-2:2017) Microbiologie de la chane alimentaire - Mth
17、ode horizontale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. - Partie 2: Mthode de dnombrement (ISO 11290-2:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren fr den Nachweis und die Zhlung von Listeria monocytogenes und von Listeria spp. - Teil 2
18、: Zhlverfahren (ISO 11290-2:2017) This European Standard was approved by CEN on 6 April 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-dat
19、e lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
20、under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Form
21、er Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATIO
22、N COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11290-2:2017 EEuropean foreword .3Introductio
23、n 51 Scope . 62 Normative references 63 Terms and definitions . 74 Principle 74.1 General . 74.2 Initial suspension 74.3 Surface plating . 74.4 Incubation . 74.5 Confirmation . 84.6 Enumeration . 85 Culture media and reagents 86 Equipment and consumables 87 Sampling 88 Preparation of test sample . 9
24、9 Procedure. 99.1 Test portion, initial suspension and dilutions 99.2 Inoculation and incubation 99.3 Enumeration of characteristic colonies . 99.4 Confirmation of L. monocytogenes or Listeria spp. . 109.4.1 Selection of colonies for confirmation . 109.4.2 Confirmation of L. monocytogenes 119.4.3 Co
25、nfirmation of Listeria spp. . 149.5 Interpretation of morphological and physiological properties and of the biochemical reactions 159.6 Additional characterization of isolated strains (optional) .1510 Expression of results .1511 Performance characteristics of the method 1511.1 Method validation stud
26、y 1511.2 Repeatability limit . 1611.3 Reproducibility limit . 1612 Test report 1613 Quality assurance .17Annex A (normative) Diagram of procedure .18Annex B (normative) Composition and preparation of media and reagents 19Annex C (informative) Distinction of Listeria spp. from other genera .27Annex D
27、 (informative) Reactions for the identification of Listeria species 28Annex E (informative) Results of interlaboratory studies for enumeration of Listeria monocytogenes .30Bibliography.33Contents PageDIN EN ISO 11290-2:2017-09 EN ISO 11290-2:2017 (E) 2Foreword 4European foreword This document (EN IS
28、O 11290-2:2017) has been prepared by Technical Committee ISO/TC 34 ” Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by pu
29、blication of an identical text or by endorsement, at the latest by December 2017, and conflicting national standards shall be withdrawn at the latest by December 2017. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN shall not
30、be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 11290-2:1998. This document has been prepared under a mandate given to CEN by the European Commission and the European Free Trade Association. According to the CEN-CENELEC Internal Regulations, the nat
31、ional standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Li
32、thuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 11290-2:2017 has been approved by CEN as EN ISO 11290-2:2017 without any modification. DIN EN ISO 1129
33、0-2:2017-09 EN ISO 11290-2:2017 (E) 3 ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body
34、interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotech
35、nical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO d
36、ocuments should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held res
37、ponsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information g
38、iven for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to
39、 Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CEN) Technical Committee CEN/TC 275, Food analysis Horizontal methods, in collaboration with ISO Technical Committee ISO/TC 34, Food products, Subcommittee
40、 SC 9, Microbiology, in accordance with the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This second edition cancels and replaces the first edition (ISO 11290-2:1998), which has been technically revised. It also incorporates the amendment ISO 11290-2:1998/Amd.1:2004. The
41、 main changes, compared to ISO 11290-2:1998, are the following. The enumeration of Listeria monocytogenes has been modified as listed below. Primary suspension with buffered peptone water, half-Fraser broth with or without supplements, and all appropriate diluents referred to in ISO 6887 (all parts)
42、. Resuscitation step deleted. Microscopic aspect, catalase and CAMP test for confirmation are optional. Inclusion of new performance characteristics. Moreover, enumeration of Listeria spp. has been included in the scope and the title changed accordingly.A list of parts in the ISO 11290 series can be
43、 found on the ISO website.DIN EN ISO 11290-2:2017-09 EN ISO 11290-2:2017 (E) 4 IntroductionThe main changes, listed in the foreword, introduced in this document compared to ISO 11290-2:1998 are considered as major (see ISO 1746828). The technical changes were assessed and were considered to have no
44、significant effect on the method performance characteristics or test results.Because of the large variety of food and feed products, this horizontal method may not be appropriate in every detail for certain products for which it may be necessary to use different or specific methods. Nevertheless, in
45、 all cases, this horizontal method is intended to be applied as far as possible and deviations from this only be made for justified technical reasons.When this document is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has
46、been followed and the reasons for deviations from it in the case of particular products.The harmonization of test methods cannot be immediate, and for certain groups of products International Standards and/or national standards may already exist that do not comply with this horizontal method. It is
47、hoped that when such standards are reviewed they will be changed to comply with this document so that eventually the only remaining departures from this horizontal method will be those necessary for well-established technical reasons.DIN EN ISO 11290-2:2017-09 EN ISO 11290-2:2017 (E) 5 WARNING In or
48、der to safeguard the health of laboratory personnel, it is essential that tests for detecting L. monocytogenes and Listeria spp. are only undertaken in properly equipped laboratories, under the control of a skilled microbiologist, and that great care is taken in the disposal of all incubated materials. Persons using this document should be familiar with normal laboratory practice. This document does not purport to address all of the safety aspects, if any, associated with its use. It is the responsi
