1、September 2011 Translation by DIN-Sprachendienst.English price group 8No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).I
2、CS 13.080.30!$taL“1816241www.din.deDDIN EN ISO 14240-1Soil quality Determination of soil microbial biomass Part 1: Substrate-induced respiration method (ISO 14240-1:1997)English translation of DIN EN ISO 14240-1:2011-09Bodenbeschaffenheit Bestimmung der mikrobiellen Biomasse von Bden Teil 1: Substra
3、t-induziertes Respirationsverfahren (ISO 14240-1:1997)Englische bersetzung von DIN EN ISO 14240-1:2011-09Qualit du sol Dtermination de la biomasse microbienne du sol Partie 1: Mthode par respiration induite par le substrat (ISO 14240-1:1997)Traduction anglaise de DIN EN ISO 14240-1:2011-09Supersedes
4、DIN ISO 14240-1:1999-10www.beuth.deDocument comprises pagesIn case of doubt, the German-language original shall be considered authoritative.1208.11 DIN EN ISO 14240-1:2011-09 2 A comma is used as the decimal marker. National foreword This standard has been prepared by Technical Committee ISO/TC 190
5、“Soil quality” in collaboration with Technical Committee CEN/TC 345 “Characterization of soils” (Secretariat: NEN, Netherlands). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-02-04 UA Biol
6、ogische Verfahren of NA 119-01-02 AA Abfall- und Bodenuntersuchung. ISO 14240 consists of the following parts, under the general title Soil quality Determination of soil microbial biomass: Part 1: Substrate-induced respiration method Part 2: Fumigation-extraction method Expert assistance and special
7、ized laboratories will be required to perform the procedure described in this standard. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 10381-6:1993 DIN ISO 10381:2009-09 ISO 10390:1994 DIN ISO 10390:2005-12 ISO 10694:1995 DIN ISO 10694
8、:1996-08 ISO 11260:1994 DIN EN ISO 11260:2011-09 ISO 11266:1994 DIN ISO 11266:1997-05 ISO 11277 DIN ISO 11277 ISO 11465:1993 DIN ISO 11465:1996-12 Amendments This standard differs from DIN ISO 14240-1:1999-10 as follows: a) ISO 14240-1 has been adopted as European Standard EN ISO 14240-1. Previous e
9、ditions DIN ISO 14240-1: 1999-10 DIN EN ISO 14240-1:2011-09 3 National Annex NA (informative) Bibliography DIN EN ISO 11260:2011-09, Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution DIN ISO 10381-6:2009-09, Soil quality Samplin
10、g Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory DIN ISO 10390:2005-12, Soil quality Determination of pH DIN ISO 10694:1996-08, Soil quality Determination of organic an
11、d total carbon after dry combustion (elementary analysis) DIN ISO 11266:1997-05, Soil quality Guidance on laboratory testing for biodegradation of organic chemicals in soil under aerobic conditions DIN ISO 11277, Soil quality Determination of particle size distribution in mineral soil material Metho
12、d by sieving and sedimentation DIN ISO 11465:1996-12, Soil quality Determination of dry matter and water content on a mass basis Gravimetric method DIN EN ISO 14240-1:2011-09 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 14240-1 June 2011 ICS 13.080.30 E
13、nglish Version Soil quality - Determination of soil microbial biomass - Part 1: Substrate-induced respiration method (ISO 14240-1:1997) Qualit du sol - Dtermination de la biomasse microbienne du sol - Partie 1: Mthode par respiration induite par le substrat (ISO 14240-1:1997) Bodenbeschaffenheit - B
14、estimmung der mikrobiellen Biomasse von Bden - Teil 1: Substrat-induziertes Respirationsverfahren (ISO 14240-1:1997) This European Standard was approved by CEN on 3 June 2011. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this Eur
15、opean Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versio
16、ns (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, B
17、elgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN C
18、OMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 14240-1:2011: EContents DIN EN
19、 ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 2 Page Foreword3 Introduction .4 1 Scope 5 2 Normative references 5 3 Definitions 5 4 Principle6 5 Test conditions .6 6 Reagents and materials 6 7 Apparatus .6 8 Procedure .6 8.1 Determination of optimum glucose concentration .6 8.2 Determination of micro
20、bial biomass .7 9 Calculation of results 7 10 Test report 7 Annex A (informative) Bibliography 8 Foreword The text of ISO 14240-1:1997 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 1424
21、0-1:2011 by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2011, and conflicting natio
22、nal standards shall be withdrawn at the latest by December 2011. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/
23、CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lith
24、uania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 14240-1:1997 has been approved by CEN as a EN ISO 14240-1:2011 without any modification. DIN EN ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 3 DIN EN ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 4 DIN EN ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 5 DIN EN ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 6 DIN EN ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 7 DIN EN ISO 14240-1:2011-09 EN ISO 14240-1:2011 (E) 8
