1、June 2014Translation by DIN-Sprachendienst.English price group 17No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13
2、.060.70!%2L“2156141www.din.deDDIN EN ISO 16665Water quality Guidelines for quantitative sampling and sample processing of marinesoft-bottom macrofauna (ISO 16665:2014);English version EN ISO 16665:2013,English translation of DIN EN ISO 16665:2014-06Wasserbeschaffenheit Anleitung fr die quantitative
3、Probenahme und Probenbearbeitung marinerWeichboden-Makrofauna (ISO 16665:2014);Englische Fassung EN ISO 16665:2013,Englische bersetzung von DIN EN ISO 16665:2014-06Qualit de leau Lignes directrices pour lchantillonnage quantitatif et le traitement dchantillons de lamacrofaune marine des fonds meuble
4、s (ISO 16665:2014);Version anglaise EN ISO 16665:2013,Traduction anglaise de DIN EN ISO 16665:2014-06SupersedesDIN EN ISO 16665:2006-02www.beuth.deIn case of doubt, the German-language original shall be considered authoritative.Document comprises 41 pages05.14 DIN EN ISO 16665:2014-06 2 A comma is u
5、sed as the decimal marker. National foreword This document (EN ISO 16665:2013) has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparatio
6、n was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-03-05-06 AK Biologisch-kologische Gews-seruntersuchung of NA 119-01-03 AA Wasseruntersuchung. In accordance with the result of the parallel voting procedure within the scope of the Agreement on te
7、chnical cooperation between ISO and CEN (Vienna Agreement), International Standard ISO 16665:2014 has been adopted as a European Standard. Designation of the method: Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna (M 50): Method DIN EN ISO 16665 M 50 This
8、standard has been published to implement Directive 2008/56/EC of the European Parliament establishing a framework for Community action in the field of marine environmental policy (Marine Strategy Framework Directive)*) and the Water Framework Directive (WFD), Directive 2000/60/EC of the European Par
9、liament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy*). The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 5667-3 DIN EN ISO 5667-3 ISO 5667-12 DIN 38414-11 ISO 5667-19 DI
10、N EN ISO 5667-19 ISO 5813 DIN EN 25813 ISO 5814 DIN EN ISO 5814 Expert assistance and specialized laboratories will be required to perform the analyses described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a
11、case-by-case basis as to whether and to what extent additional conditions will have to be specified. This standard has been prepared by the Normenausschuss Wasserwesen (Water Practice Standards Com-mittee) in collaboration with the Wasserchemische Gesellschaft Fachgruppe in der Gesellschaft Deutsche
12、r Chemiker (Water Chemistry Society Division of the German Chemical Society). It is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung (German standard methods for the examination of water, waste water and sludge): Guidelines for quantitative sampling and sa
13、mple processing of marine soft-bottom macrofauna (M 50). *) Registered in the DITR database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 10772 Berlin. DIN EN ISO 16665:2014-06 3 Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or gro
14、uped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheits-verfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Beuth Verlag GmbH and Wiley-VCH Verlag GmbH b) strategies and objectives for baseline surveys or environme
15、ntal impact assessments and temporal trend monitoring have been revised; c) sample fixation requirements have been revised; d) Annex A “Sampling devices” has been revised and supplemented; e) Annex C “Biomass measurements” has been revised and supplemented; f) Annex B “Processing of samples with liv
16、e specimens” has been added; g) the standard has been editorially revised. Previous editions DIN EN ISO 16665: 2006-02 DIN EN ISO 16665:2014-06 4 National Annex NA (informative) Bibliography DIN 38414-11, German standard methods for the examination of water, waste water and sludge; sludge and sedime
17、nts (group S) Sampling of sediments (S 11) DIN EN 25813, Water quality Determination of dissolved oxygen Iodometric method DIN EN ISO 5667-19, Water quality Sampling Part 19: Guidance on sampling of marine sediments DIN EN ISO 5667-3, Water quality Sampling Part 3: Preservation and handling of water
18、 samples DIN EN ISO 5814, Water quality Determination of dissolved oxygen Electrochemical probe method EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO16665December 2013 ICS 13.060.70; 13.060.10 Supersedes EN ISO 16665:2005English Version Water quality - Guidelines for quantitative sampling
19、and sample processing of marine soft-bottom macrofauna (ISO 16665:2014)Qualit de leau - Lignes directrices pour lchantillonnage quantitatif et le traitement dchantillons de la macrofaune marine des fonds meubles (ISO 16665:2014) Wasserbeschaffenheit - Anleitung fr die quantitative Probenahme und Pro
20、benbearbeitung mariner Weichboden-Makrofauna (ISO 16665:2014) This European Standard was approved by CEN on 6 December 2013. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard wi
21、thout any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any othe
22、r language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic,
23、Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMI
24、TTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGCEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16665:2013 EContents Page
25、 Foreword . 3 Introduction . 4 1 Scope . 5 2 Terms and definitions 5 2.1 Ecological or biological concepts 6 2.2 Surveys and samples. 6 3 Strategies and objectives for soft-bottom faunal surveys. 7 3.1 Sampling programme and plan 7 3.2 Positioning of sampling stations 7 3.3 Reference stations . 9 3.
26、4 Types of survey 9 3.5 Change in sampling programme and intercalibration 12 4 Sampling . 12 4.1 Documentation and field log . 12 4.2 Sampling and sample processing in the field . 14 4.3 Sample fixation . 17 4.4 Background environmental descriptors 18 5 Sample processing in the laboratory . 21 5.1 S
27、orting . 21 5.2 Sample residue . 22 6 Taxon determination and quantification 22 6.1 Level of identification and taxon lists 22 6.2 Quantification . 23 6.3 Reference collection 23 6.4 Biomass 24 6.5 Data reporting . 24 6.6 Storage and archiving 24 7 Quality assurance and quality control . 25 7.1 Gene
28、ral 25 7.2 Auditing . 25 7.3 Equipment calibration and operating safety . 25 7.4 Training . 26 7.5 Checklists, sample log and anomaly reporting 26 7.6 Quality of sample sorting 26 7.7 Quality of taxon identification . 26 7.8 Identification literature. 27 7.9 Reference and collection . 27 Annex A (in
29、formative) Sampling devices 28 Annex B (informative) Processing of samples with live specimens 33 Annex C (informative) Biomass measurements . 34 Annex D (informative) Processing particularly large samples 36 Bibliography 37 2DIN EN ISO 16665:2014-06EN ISO 16665:2013 (E)ForewordThis document (EN ISO
30、 16665:2013) has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identic
31、al text or by endorsement, at the latest by June 2014, and conflicting national standards shall be withdrawn at the latest by June 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsi
32、ble for identifying any or all such patent rights. This document supersedes EN ISO 16665:2005. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus
33、, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United K
34、ingdom. Endorsement notice The text of ISO 16665:2014 has been approved by CEN as EN ISO 16665:2013 without any modification. 3DIN EN ISO 16665:2014-06EN ISO 16665:2013 (E)IntroductionAnalysis of macrofaunal communities in soft-bottom sediments is an integral part of marine environmental assessment.
35、 The faunal composition, in terms of both the species present and their relative abundance, reflects integrated environmental conditions in the survey area over a period of time. The composition and structure of soft-bottom macrofaunal communities therefore can be used to characterize environmental
36、conditions and estimate the extent of environmental impact.Characterization of environmental conditions is usually based on quantitative methods, in this case by relating the numbers of species and individuals captured to a known area of sea floor. For accurate data interpretation, it is essential t
37、o add information on the geophysicochemical characteristics or properties of the water masses and bottom sediments, including nutrients, oxygenation, and redox state where appropriate.For effective data utilization and quality assurance (QA) of the work carried out, it is beneficial and may be essen
38、tial (depending on the individual survey aims) that surveys be intercomparable temporally, spatially, and between operators. This International Standard contributes to ongoing work on QA of data from soft-bottom macrofaunal surveys. These guidelines primarily aim to assist in standardizing monitorin
39、g surveys carried out for commercial purposes or in connection with the EU Water Framework Directive. For this reason, detailed specifications are given in areas of consequence for data intercompatibility.Where appropriate, costbenefit issues have been taken into consideration, and accepted minimal
40、requirements for general environmental impact assessment have been given. The cited minimum requirements for accuracy are not intended to satisfy research needs or to provide a full ecological understanding of the sampling area. Designers of programmes for research or other studies requiring a detai
41、led knowledge of soft-bottom macrofauna should consult the guidelines given in Reference 13 for decisions on survey design and sampling frequency.This International Standard applies to all areas of the sea floor where it is possible to collect faunal samples by a grab or coring device. For practical
42、 reasons, this applies to animals retained on a mesh screen of 0,5 mm or 1 mm aperture size.The sensitivity of the method, here defined as detection of faunal disturbance, change in taxon composition or faunal mapping, is dependent on the survey design, the type of environmental influences present i
43、n the area and on the level of competence or standardization of the personnel.4DIN EN ISO 16665:2014-06EN ISO 16665:2013 (E)WARNING Persons using this document should be familiar with normal laboratory practice. This document does not purport to address all of the safety problems, if any, associated
44、 with its use. It is the responsibility of the employer or user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions.IMPORTANT It is absolutely essential that tests conducted in accordance with this document be carried out by suitably
45、trained staff.1 ScopeThis International Standard provides guidelines on the quantitative collection and processing of subtidal soft-bottom macrofaunal samples in marine waters.This International Standard encompasses:a) development of the sampling programme;b) requirements for sampling equipment;c) s
46、ampling and sample treatment in the field;d) sorting and species identification;e) storage of collected and processed material.This International Standard does not specifically address the following, although some elements may be applicable: bioassay sub-sampling; deep water (750 m) or offshore samp
47、ling; in situ faunal studies, e.g. recolonization assays; non-benthic organisms caught in the sampling device; estuarine sampling; intertidal sampling; meiofaunal sampling and analysis (see Reference 9); sampling by dredge and sledge; self-contained underwater breathing apparatus (SCUBA) sampling; s
48、tatistical design.Accuracy of position fixing is determined by the geographical area, equipment used and survey objective.2 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.5DIN EN ISO 16665:2014-06EN ISO 16665:2013 (E)2.1 Ecological or biological concepts2.1.1benthicdwelling at the bottom of an aquatic environment2.1.2benthic macrofaunabottom-dwelling animals retained on a mesh screen of 0,5 mm or 1 mm aperture size2.1.3receiving water bodyrecipie
