1、March 2013 Translation by DIN-Sprachendienst.English price group 12No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS
2、07.100.20; 13.060.30; 13.060.70!$#2“1970015www.din.deDDIN EN ISO 7827Water quality Evaluation of the “ready“, “ultimate“ aerobic biodegradability of organiccompounds in an aqueous medium Method by analysis of dissolved organic carbon (DOC) (ISO 7827:2010);English version EN ISO 7827:2012,English tra
3、nslation of DIN EN ISO 7827:2013-03Wasserbeschaffenheit Bestimmung der “leichten“, “vollstndigen“ aeroben biologischen Abbaubarkeitorganischer Stoffe in einem wssrigen Medium Verfahren mittels Analyse des gelsten organischen Kohlenstoffs (DOC) (ISO 7827:2010);Englische Fassung EN ISO 7827:2012,Engli
4、sche bersetzung von DIN EN ISO 7827:2013-03Qualit de leau valuation de la biodgradabilit arobie facile, ultime des composs organiques enmilieu aqueux Mthode par analyse du carbone organique dissous (COD) (ISO 7827:2010);Version anglaise EN ISO 7827:2012,Traduction anglaise de DIN EN ISO 7827:2013-03
5、SupersedesDIN EN ISO 7827:1996-04www.beuth.deDocument comprises 22 pagesIn case of doubt, the German-language original shall be considered authoritative.02.13DIN EN ISO 7827:2013-03 2 A comma is used as the decimal marker. National foreword The text of ISO 7827:2010 has been prepared by Technical Co
6、mmittee ISO/TC 147 “Water quality” and has been taken over as EN ISO 7827:2012 by Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Com
7、mittee NA 119-01-03-05-01 AK Biotests of NA 119-01-03 AA Wasseruntersuchung. Designation of the method: Evaluation of the “ready”, “ultimate” aerobic biodegradability of organic compounds in an aqueous medium Method by analysis of dissolved organic carbon (DOC) (L 29): Method DIN EN ISO 7827 L 29 Th
8、is standard has been published to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy1). The DIN Standards corresponding to the International St
9、andards referred to in this document are as follows: ISO 8192 DIN EN ISO 8192 ISO 8245 DIN EN 1484 ISO 9408 DIN EN ISO 9408 ISO 9439 DIN EN ISO 9439 ISO 9887 DIN EN ISO 9887 ISO 9888 DIN EN ISO 9888 ISO 10707 DIN EN ISO 10707 ISO 14593 DIN EN ISO 14593 ISO/TR 15462 DIN SPEC 1164 (DIN-Fachbericht ISO
10、/TR 15462) Expert assistance and specialized laboratories will be required to perform the analyses described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what extent a
11、dditional conditions will have to be specified. This standard has been prepared by the Normenausschuss Wasserwesen (Water Practice Standards Committee) in collaboration with the Wasserchemische Gesellschaft Fachgruppe in der Gesellschaft Deutscher Chemiker (Water Chemistry Society Division of the Ge
12、rman Chemical Society). It is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung (German standard methods for the examination of water, waste water and sludge): 1) Registered in the DITR database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 1077
13、2 Berlin.DIN EN ISO 7827:2013-03 3 Evaluation of the “ready”, “ultimate” aerobic biodegradability of organic compounds in an aqueous medium Method by analysis of dissolved organic carbon (DOC). Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or
14、grouped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag GmbH b) Clause 1 “Scope” has been extended: it has been clarified that the method is no
15、t suitable for waste waters; reference is made to the definitions of the terms “ready” and “ultimate” in this standard; c) Clause 3 “Terms and definitions” has been extended: degradation time (3.1) inherent biodegradation (3.2); lag time (3.3); maximum level of biodegradation (3.4); “ready“ biodegra
16、dation (3.6); suspended solids (3.7); the definition of the term primary biodegradation (3.5) has been modified; d) Clause 4 “Principle” has been extended to include: information on incubation conditions (aeration, lighting, temperature); information on the use of inhibitory substances; information
17、that the test conditions specified do not necessarily correspond to the optimal conditions allowing the maximum degree of biodegradation to occur; indication of the very stringent requirements to fulfil the criterion of ready biodegradability; reference to alternative biodegradation methods; e) in C
18、lause 5 “Test environment”, the temperature range for the incubation has been changed to 20 C to 24 C (instead of 20 C to 25 C); f) Clause 6 “Reagents”: the permissible pH value of solution A has been changed to 7,4 0,2 (instead of 7,4); Subclause 6.2.2 “Preparation” has been extended to clarify the
19、 order in which the solutions are added and how long they can be stored; g) Clause 7 “Apparatus and materials”: 7.1 has been supplemented to indicate the measurement range of the DOC analysis apparatus; 7.2. has been supplemented to indicate the centrifuge to be used; 7.4 has been supplemented to sp
20、ecify the environment for incubation; DIN EN ISO 7827:2013-03 5 h) Clause 8 “Procedure” has been modified: description of the procedure when adding substances of low solubility in 8.1.1; assessment to be carried out when using a pre-exposed inoculum; the requirement to wash the inoculum has been tra
21、nsposed to 8.2.3, this remains an important step; the cell concentration of the inoculum has been rendered more precise by specifying the cell concentration in the final mixture; an alternative method for the preparation of an inoculum from homogenized sludge has been added; Subclause 8.3 “Test proc
22、edure” has been divided into 8.3.1 “Preparation of test and control vessels” and 8.3.2 “Incubation and sampling”; filter sterilization has been included as an alternative to the flask used for checking the abiotic removal in 8.3.1 e) by means of chemical substances preventing microbial activity; the
23、 former notes 5 and 6 relating to the elimination or adsorption in the flasks are now incorporated in the normative text; the permissible temperature range for the incubation has been changed to (20 2) C (instead of (20 to 25) C; for flasks FT, FB, FC, FI, the volume of the inoculum to be added has
24、been changed to 10 ml to 100 ml for 1 000 ml of test solution (instead of 1 ml to 10 ml); the information has been added that measurements at shorter intervals and more sampling points near the start of the test are necessary to identify the 10 d windows; the centrifugation time has been changed fro
25、m 15 min to 25 min; the temperature range for the storage of samples used for the measurements of organic carbon has been changed to 18 C (instead of 18 C); i) Clause 9 “Calculation and expression of results”: Subclause 9.2 “Calculation of primary biodegradation” has been included; the calculation o
26、f the abiotical removal and for the elimination of the reference substance has been incorporated in the normative text; reference is made to Annex B for further information on the interpretation of results; j) Clause 10 “Validity of the test”: it has been specified that the DOC contributed to the te
27、st system by the inoculum is less than 10 % of that contributed by the test compound; Annex B (informative) “Interpretation of results” has been included; k) normative references and the Bibliography have been updated and the standard has been editorially revised. Previous editions DIN EN ISO 7827:
28、1996-04 DIN EN ISO 7827:2013-03 6 National Annex NA (informative) Bibliography DIN EN 1484, Water analysis Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC) DIN EN ISO 8192, Water quality Test for inhibition of oxygen consumption by activated sludge fo
29、r carbonaceous and ammonium oxidation DIN EN ISO 9408, Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer DIN EN ISO 9439, Water quality Evaluation of ultimate aerobic biodegradability of org
30、anic compounds in aqueous medium Carbon dioxide evolution test DIN EN ISO 9887, Water quality Evaluation of the aerobic biodegradability of organic compounds in an aqueous medium Semi-continuous activated sludge method (SCAS) DIN EN ISO 9888, Water quality Evaluation of ultimate aerobic biodegradabi
31、lity of organic compounds in aqueous medium Static test (Zahn-Wellens method) DIN EN ISO 10707, Water quality Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds Method by analysis of biochemical oxygen demand (closed bottle test) DIN EN ISO 14593, Water q
32、uality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium Method by analysis of inorganic carbon in sealed vessels (CO2headspace test) DIN SPEC 1164 (DIN-Fachbericht ISO/TR 15462), Water quality Selection of tests for biodegradability EUROPEAN STANDARD NORME EUROP
33、ENNE EUROPISCHE NORM EN ISO 7827 December 2012 ICS 13.060.70 Supersedes EN ISO 7827:1995English Version Water quality - Evaluation of the “ready“, “ultimate“ aerobic biodegradability of organic compounds in an aqueous medium -Method by analysis of dissolved organic carbon (DOC) (ISO 7827:2010) Quali
34、t de leau - valuation de la biodgradabilit arobie “facile“, “ultime“ des composs organiques en milieu aqueux - Mthode par analyse du carbone organique dissous (COD) (ISO 7827:2010) Wasserbeschaffenheit - Bestimmung der leichten , vollstndigen aeroben biologischen Abbaubarkeit organischer Stoffe in e
35、inem wssrigen Medium - Verfahren mittels Analyse des gelsten organischen Kohlenstoffs (DOC) (ISO 7827:2010) This European Standard was approved by CEN on 8 December 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
36、 Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (E
37、nglish, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgiu
38、m, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerl
39、and, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref.
40、 No. EN ISO 7827:2012: E“ “ “Contents Page Foreword .3 1 Scope4 2 Normative references4 3 Terms and definitions .5 4 Principle .6 5 Test environment.6 6 Reagents 6 7 Apparatus.7 8 Procedure.8 9 Calculation and expression of results 10 10 Validity of the test11 11 Test report12 Annex A (informative)
41、Typical degradation curve .13 Annex B (informative) Interpretation of results .14 Bibliography16 DIN EN ISO 7827:2013-03 EN ISO 7827:2012 (E) 2Foreword The text of ISO 7827:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization
42、(ISO) and has been taken over as EN ISO 7827:2012 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2
43、013, and conflicting national standards shall be withdrawn at the latest by June 2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights.
44、 This document supersedes EN ISO 7827:1995. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former
45、 Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 7827:201
46、0 has been approved by CEN as a EN ISO 7827:2012 without any modification. DIN EN ISO 7827:2013-03 EN ISO 7827:2012 (E) 3 WARNING Persons using this International Standard should be familiar with normal laboratory practice. This standard does not purport to address all of the safety problems, if any
47、, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted according to this International Standard be carried out by
48、suitably trained staff. SAFETY PRECAUTIONS Activated sludge and sewage contain potentially pathogenic organisms. Therefore take appropriate precautions when handling them. Handle toxic test compounds and those whose properties are unknown with care. 1 Scope This International Standard specifies a me
49、thod for the evaluation of the “ready” and “ultimate” biodegradability of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, this International Standard also gives specific definitions for the terms “ready” and “ultimate”. The method applies to organic compounds which are: a) soluble at the c
