1、May 2007DEUTSCHE NORM English price group 13No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.060.50!,xb1“9856314www.
2、din.deDDIN EN ISO 8192Water quality Test for inhibition of oxygen consumption by activated sludge forcarbonaceous and ammonium oxidation (ISO 8192:2007)English version of DIN EN ISO 8192:2007-05Wasserbeschaffenheit Bestimmung der Hemmung des Sauerstoffverbrauchs von Belebtschlamm nachKohlenstoff- un
3、d Ammonium-Oxidation (ISO 8192:2007)Englische Fassung DIN EN ISO 8192:2007-05SupersedesDIN EN ISO 8192:1995-04www.beuth.deDocument comprises 29 pages 08.07DIN EN ISO 8192:2007-05 2 This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlamm-untersuchung Testverf
4、ahren mit Wasserorganismen (Gruppe L) (German standard methods for the examination of water, waste water and sludge Test methods using water organisms (group L) and describes method L 39. National foreword This standard has been prepared by Technical Committee ISO/TC 147 “Water quality” in collabora
5、tion with Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Technical Committees NA 119-01-03-05-01 AK Biotests and NA 119-01-03 AA Wasserunter
6、suchung. Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Existing safety regulations are to be taken into account. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what e
7、xtent additional conditions will have to be specified. Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser-
8、und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth Verlag GmbH. All standard methods relevant to the Abwasserverordnung (Waste Water Regulation) (AbwV) included in the new Regulation on Section 7a of the Gesetz zur Ordnung des Wasserhaushalts (German Water Management
9、 Act) together with the Abwasserverordnung and the Gesetz zur Ordnung des Wasserhaushalts and other valid administrative regulations on waste water have been published as a loose-leaf compilation “Analysen-verfahren in der Abwasserverordnung Rechtsvorschriften und Normen”*). Standard methods or draf
10、t standards bearing the group title “German standard methods for the examination of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory analysis (group B) (DIN 38403) Physical and physicochemical parameters (gr
11、oup C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 38406) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) Biological-ecological methods of analysis (group M) (DIN 3841
12、0) Microbiological methods (group K) (DIN 38411) Test methods using water organisms (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) *)Available in German. DIN EN ISO 8192:2007-05 3 In
13、addition to the methods described in the DIN 38402 to DIN 38415 series of standards, there are a number of European and International Standards available as DIN EN, DIN EN ISO and DIN ISO Standards, which also form part of the collection of German standard methods. Information on Parts of these seri
14、es of standards that have already been published can be obtained from the offices of the Normenausschuss Wasserwesen, telephone +49 30 2601-2448, or from Beuth Verlag GmbH, Burggrafenstrae 6, 10787 Berlin. The DIN Standards corresponding to the International Standards referred to in the EN are as fo
15、llows: ISO 3696 DIN ISO 3696 ISO 5667-16 DIN EN ISO 5667-16 ISO 5814 DIN EN 25814 ISO 9509 DIN EN ISO 9509 ISO 9887 DIN EN ISO 9887 ISO 9888 DIN EN ISO 9888 ISO 10634 DIN EN ISO 10634 ISO 11733 DIN EN ISO 11733 ISO 11923 DIN EN 872 Amendments This standard differs from DIN EN ISO 8192:1995-04 as fol
16、lows: a) The title has been changed. b) Antifoam agents, an example of an inhibition curve, and a suitable system for preparing nitrifying activated sludge in the laboratory have been included in the annexes. c) Procedure and evaluation of the analytical method have been amended. d) The standard has
17、 been editorially revised. Previous editions DIN EN ISO 8192: 1995-04 National Annex NA (informative) Bibliography DIN EN 872, Water quality Determination of suspended solids Method by filtration through glass fibre filters DIN EN ISO 5667-16, Water quality Sampling Part 16: Guidance on biotesting o
18、f samples DIN EN 25814, Water quality Determination of dissolved oxygen by the electrochemical probe method DIN EN ISO 8192:2007-05 4 DIN EN ISO 9509, Water quality Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms DIN EN ISO 9887, Water quality Evaluatio
19、n of the aerobic biodegradability of organic compounds in an aqueous medium Semi-continuous activated sludge method (SCAS) DIN EN ISO 9888, Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium Static test (Zahn-Wellens method) DIN EN ISO 10634, Water q
20、uality Guidance for the preparation and treatment of poorly watersoluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium DIN EN ISO 11733, Water quality Determination of the elimination and biodegradability of organic compounds in an aqueous medium Acti
21、vated sludge simulation test DIN EN ISO 3696, Water for analytical laboratory use Specification and test methods EUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 8192February 2007ICS 13.060.70 Supersedes EN ISO 8192:1995 English VersionWater quality - Test for inhibition of oxygen consumption b
22、yactivated sludge for carbonaceous and ammonium oxidation(ISO 8192:2007)Qualit de leau - Essai dinhibition de la consommationdoxygne par des boues actives pour loxydation ducarbone et de lammonium (ISO 8192:2007)Wasserbeschaffenheit - Bestimmung der Hemmung desSauerstoffverbrauchs von Belebtschlamm
23、nachKohlenstoff- und Ammonium-Oxidation (ISO 8192:2007)This European Standard was approved by CEN on 29 December 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without an
24、y alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by
25、 translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germ
26、any, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGMana
27、gement Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 8192:2007: EContents Page Foreword Introduction 1 Scope . 2 Normative references . 3 Terms and definitions. 4 Principle. 5 Re
28、agents, media and inoculum. 6 Apparatus 7 Test environment 8 Procedure 9 10 Validity of the results 11 Test report .Annex A (informative) Examples of measuring units .Annex B (informative) Apparatus for culturing nitrifying activated sludge .Annex C (informative) Overview of the test procedure Annex
29、 D (informative) Mixtures for the preliminary test Annex E (informative) Example of an inhibition curve .Bibliography .2 EN ISO 8192:2007 (E) . 34 55567899121517 18 20222324 25Calculation and expression of resultsForeword This document (EN ISO 8192:2007) has been prepared by Technical Committee ISO/
30、TC 147 “Water Quality” in collaboration with Technical Committee CEN/TC 230 “Water Analysis”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by August 2007,
31、and conflicting national standards shall be withdrawn at the latest by August 2007. This document supersedes EN ISO 8192:1995. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Be
32、lgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice T
33、he text of ISO 8192:2007 has been approved by CEN as EN ISO 8192:2007 without any modifications. 3 EN ISO 8192:2007 (E) Introduction Information generated by this method for assessing the potential toxicity of substances, mixtures and waste waters to activated sludge may be helpful in estimating the
34、 effect of a test material on mixed bacterial communities in the aquatic environment, especially in aerobic biological treatment systems. The susceptibility of oxygen uptake by different sub-populations of the bacterial communities to inhibition by chemicals and waste waters is not necessarily unifo
35、rm and selective effects may profoundly influence the outcome of the test. There are two principal groups of microorganisms contributing to the total oxygen consumption by activated sludge: heterotrophic organisms mainly responsible for the breakdown of carbon-based substrates (carbonaceous oxidatio
36、n) and autotrophic nitrifying organisms causing the oxidation of ammonium to nitrate (nitrification). This International Standard may be used to assess the toxicity of substances on total oxygen uptake (i.e. carbonaceous oxidation and nitrification combined) or, by deliberately adding a specific inh
37、ibitor of nitrification, also to assess toxicity of substances to the carbonaceous and nitrification components separately. For the determination of the nitrification inhibition with this method, a sufficiently nitrifying activated sludge is required. Indications of nitrification may be investigated
38、 further by application of ISO 95094. The user of this method should be aware that particular problems could require the specification of additional marginal conditions. The inhibitory effect of a test material may be exerted on both components or it may be exerted predominantly on only one of them.
39、 Nitrification is the process more commonly prone to selective inhibition. 4 EN ISO 8192:2007 (E) WARNING Persons using this International Standard should be familiar with normal laboratory practice. This International Standard does not purport to address all of the safety problems, if any, associat
40、ed with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted according to this International Standard be carried out by suitably t
41、rained staff. 1 Scope This International Standard specifies a method for assessing the inhibitory effect of a test material on the oxygen consumption of activated sludge microorganisms. This method is intended to represent the conditions in biological waste-water treatment plants. It gives informati
42、on on inhibitory or stimulatory effects after a short exposure (usually 30 min up to 180 min or even more) of the test material on activated sludge microorganisms. This method is applicable for testing waters, waste waters, pure chemicals and mixtures of chemicals. Concerning the chemicals, the meth
43、od refers to those which are soluble under the test conditions. Special care is necessary with materials of low water solubility, high volatility and with materials abiotically consuming or producing oxygen. 2 Normative references The following referenced documents are indispensable for the applicat
44、ion of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 3696, Water for analytical laboratory use Specification and test methods 3 Terms and definitions For the purposes
45、of this document, the following terms and definitions apply. 3.1 activated sludge accumulated biological mass (floc) produced in the treatment of waste water by the growth of bacteria and other microorganisms in the presence of oxygen (ISO 6107-1:2004 3, definition 2) 3.2 concentration of suspended
46、solids of an activated sludge amount of solids obtained by filtration or centrifugation of a known volume of activated sludge and drying at about 105 C to constant mass (ISO 9888:1999 6, definition 3.4) 5 EN ISO 8192:2007 (E) 3.3 oxygen consumption rate uptake of oxygen by activated sludge microorga
47、nisms per unit volume of sludge, in unit time NOTE This quantity is expressed in milligrams per litre per hour mg/(lh). 3.4 specific oxygen consumption rate uptake of oxygen by activated sludge microorganisms per unit mass of dry sludge (suspended solids), in unit time NOTE This quantity is expresse
48、d in milligrams per gram per hour mg/(gh). 3.5 inhibition of oxygen consumption decrease of the oxygen consumption rate of an activated sludge plus (a) degradable substance(s) in the presence of the test material, compared with that of a similar mixture without test material NOTE 1 This quantity is
49、expressed as a percentage. NOTE 2 In the absence of a substrate, some chemicals (e.g. uncouplers of phosphorylation) can increase oxygen uptake. 3.6 toxic range range of concentration of a test material over which 0 % to 100 % inhibition occurs 3.7 EC50effective concentration of the test material giving a calculated or interpolated inhibition of oxygen consumption of 50 % compared with a blank control 3.8 nitrification oxidation of ammonium compounds by bacteria NOTE Usually the intermediate product is nitrit
