1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationWood preservatives Determination of toxic values against Reticulitermesspecies (European termites)(Laboratory method)BS EN 117:2012National forewordThis British Standard is the U
2、K implementation of EN 117:2012. It supersedes BS EN 117:2005 which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee B/515, Wood preservation.A list of organizations represented on this committee can be obtained on request to its secretary.This publication do
3、es not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2012.Published by BSI Standards Limited 2012 ISBN 978 0 580 77208 5 ICS 71.100.50Compliance with a British Standard cannot confer immunity from l
4、egal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 November 2012.Amendments issued since publicationDate Text affectedBRITISH STANDARDBS EN 117:2012EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 117 October 2012 ICS 71.
5、100.50 Supersedes EN 117:2005English Version Wood preservatives - Determination of toxic values against Reticulitermes species (European termites) (Laboratory method)Produit de prservation du bois - Dtermination du seuil defficacit contre les termites europens du genre Reticulitermes (Mthode de labo
6、ratoire) Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenber Reticulitermes-Arten (Europische Termiten) (Laboratoriumsverfahren) This European Standard was approved by CEN on 24 September 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate th
7、e conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard
8、 exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the nationa
9、l standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovak
10、ia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved wor
11、ldwide for CEN national Members. Ref. No. EN 117:2012: EBS EN 117:2012EN 117:2012 (E) 2 Contents Page Foreword 3 Introduction .4 1 Scope 5 2 Normative reference 5 3 Terms and definitions .5 4 Principle 5 5 Test materials .6 6 Sampling .7 7 Test specimens 8 8 Procedure .9 9 Expression of results . 16
12、 10 Test report . 16 Annex A (informative) Example of a test report 18 Annex B (informative) Example of a method of culturing termites . 20 Annex C (informative) Environmental, health and safety precautions within chemical/biological laboratory 21 Bibliography . 22 BS EN 117:2012EN 117:2012 (E) 3 Fo
13、reword This document (EN 117:2012) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by end
14、orsement, at the latest by April 2013, and conflicting national standards shall be withdrawn at the latest by April 2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identif
15、ying any or all such patent rights. This document supersedes EN 117:2005. Significant technical differences between this document and EN 117:2005 are as follows: a) the number of treated test specimens was changed to at least five test specimens for each concentration of the product; b) the limiting
16、 values to determine the toxic values of a preservative were changed. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark
17、, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. BS EN 117:2012EN
18、117:2012 (E) 4 Introduction This document describes a laboratory method of testing which gives a basis for assessment of the effectiveness of a wood preservative against the Reticulitermes species of European termites. It allows the determination of the concentration at which the product completely
19、prevents attack by these insects of impregnated wood of a susceptible species. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative can be applied should be taken into account. It is further rec
20、ommended that results from this should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as
21、 possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex C for
22、 environmental, health and safety precautions). BS EN 117:2012EN 117:2012 (E) 5 1 Scope This European Standard specifies a method for the determination of the toxic values of a wood preservative against the Reticulitermes species of European termites1). This method is applicable to: water-insoluble
23、chemicals which are being studied as active insecticides; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and water-soluble materials, for example salts. NOTE This method can be used in conjunction with an ageing procedure, for example
24、 EN 73 or EN 84. 2 Normative reference The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (inc
25、luding any amendments) applies. EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical characteri
26、stics identical to the volumetric average characteristics of the total volume being sampled 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) 4 Principle Impregnation of several sets of test specimens of susceptible wood with a series of solu
27、tions in which the concentration of preservative is ranged in a given progression. Exposure of these test specimens to specified colonies of Reticulitermes2)and assessment of the attack suffered after exposure under fixed conditions and over a fixed period. 1) The method can be applied not only to d
28、ifferent species of Reticulitermes, but also to other species of the family of the Rhinotermitidae, adapting the conditions of temperature and humidity where necessary to the specific requirements of the species concerned. 2) In providing biological validation of individual species, it is essential
29、that the locality of origin of each test termite species is given. The description of the locality should at least include the district name. BS EN 117:2012EN 117:2012 (E) 6 Comparison of these results with those obtained with untreated and solvent or diluent-treated control test specimens. Derivati
30、on of the toxic values of the product under test. 5 Test materials 5.1 Biological material Workers, soldiers and nymphs of an identified termite species of Reticulitermes. The termite species and the locality of origin should be stated in the test report and their identification should be proved. Th
31、e termites should be obtained from colonies reared as described in Annex B. 5.2 Products and reagents 5.2.1 Substrate for establishing the colonies. A choice of: 5.2.1.1 Fine white quartz sand consisting of grains of crystallised silica, very pure (99,5 % silica), and free from any organic substance
32、s 3). 5.2.1.2 An hydrated, laminar, aluminium-iron-magnesium silicate exfoliated to give particles of 1 mm to 3 mm with an apparent density of 80 kg/m3to 90 kg/m3. Particles of less than 1 mm shall be eliminated by sieving prior to use to ensure the absence of free water and prevent any significant
33、agglomeration of the particles. 5.2.1.3 Rigid polyurethane foam with open pores of mass per unit of volume of 14 kg/m3and compressive strength 4)of 0,02 N/mm2to 0,03 N/mm2. It is advisable to cut the foam into sheets 15 mm thick. 5.2.2 Fumigant (if necessary) xylene, technical grade, mixed isomers.
34、5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a suitable volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood which would have a toxic effect on the insect at the end of the conditioning period. 5.2.5 Filter paper, ord
35、inary quality, medium-fast grade. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (26 2) C and at a minimum relative humidity of (70 5) %. 5.3.2 Conditioning chamber, well ventilated, controlled at (20 2) C and relative humidity (65 5) %5). 3) In France Fontainebleau sand,
36、 of which more than 97 % of the particles are between 75 m and 300 m in size, provides these features. 4) Determined in accordance with EN IS0 844. BS EN 117:2012EN 117:2012 (E) 7 5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out6). 5.3.4 Testing chamb
37、er, protected from light, ventilated and controlled at (26 2) C and at a minimum relative humidity of (70 5) %. 5.3.5 Treatment vessels, of a material that does not react with the preservative under test, for example of glass for organic products and of polyethylene for salts containing fluorine. 5.
38、3.6 Drying vessel(s), capable of holding sets of three test specimens (7.5), provided with a close-fitting cover and containing supports that will give minimum contact with treated test specimens to be placed on them. The vessels and supports shall be of a material that does not react with the prese
39、rvative under test, for example glass for organic compounds and polyethylene for products containing fluorine. 5.3.7 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come in contact during the test. 5.3.8 Safety equipment and protecti
40、ve clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.9 Vacuum vessels, fitted with stopcocks, capable of receiving the treatment. 5.3.10 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa. 5.3.11 Instrume
41、nts, adapted for termite manipulation (aspirator, forceps). 5.3.12 Test containers, suitable for holding the test specimens and of material resistant to the product used, and fitted with perforated cover to provide a good exchange of air. Base area 35 cm2to 60 cm2Minimum height 8,5 cm Volume 500 cm3
42、to 1 000 cm35.3.13 Glass rings, 20 mm high, 20 mm in diameter and with a wall thickness of at least 1 mm. 5.3.14 Protective gloves 5.3.15 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g. 6 Sampling The sample of preservative shall be representative of
43、the product to be tested. Samples should be stored and handled in accordance with any written recommendations from the supplier. NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 will be used. 5) The conditioning of test specimens after treatment can be carried
44、 out in the laboratory work area (5.3.3) provided that this meets the conditions specified for the conditioning chamber (5.3.2). 6) It is essential to follow safety procedures for handling flammable and toxic materials. Excessive exposure of operators to solvents or their vapours should be avoided.
45、BS EN 117:2012EN 117:2012 (E) 8 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus). Additional tests can be carried out using other species but, if so, this should be stated in the test report. 7.2 Wood quality The wood shall be free from visible cra
46、cks, stain, decay, insect damage and other defects. The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in winter. NOTE Wood that has been kiln dried at temperatures below 60 C can be used. The wood shall be exclusi
47、vely sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole. It is recommended to use test specimens of similar growth rate within a single test. 7.3 Provision o
48、f test specimens Prepare planed strips having a cross-section of (25 0,5) mm x (15 0,5) mm removing a minimum of 2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall have a contact angle of greater than 10 to the
49、 broad faces of the test specimens. Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end grain surfaces, to give test specimens (50 0,5) mm long. The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock originally of more than 500 test specimens. 7.4 Dimensions of test specimens The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be (50 0,5) mm x (25 0,5) mm x (15 0,5) mm. For the purposes of c
