1、BRITISH STANDARD BS EN 14119:2003 Testing of textiles Evaluation of the action of microfungi The European Standard EN 14119:2003 has the status of a British Standard ICS 07.100.99; 50.080.30 BS EN 14119:2003 This British Standard was published under the authority of the Standards Policy and Strategy
2、 Committee on 14 October 2003 BSI 14 October 2003 ISBN 0 580 42768 4 National foreword This British Standard is the official English language version of EN 14119:2003. It supersedes BS 6085:1992 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee TCI/80,
3、Textiles Chemical testing, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in th
4、e BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible
5、for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK i
6、nterests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN title page, pages 2 to 21 and a back cover. The BSI copyright notice displayed in this document indicates wh
7、en the document was last issued. Amendments issued since publication Amd. No. Date CommentsEUROPEANSTANDARD NORMEEUROPENNE EUROPISCHENORM EN14119 September2003 ICS07.100.99;59.080.30 Englishversion TestingoftextilesEvaluationoftheactionofmicrofungi EssaissurlestextilesEvaluationdelactiondes champign
8、onsmicroscopiques PrfungvonTextilienBestimmungderEinwirkung mikroskopischerPilze(Mikrofungi) ThisEuropeanStandardwasapprovedbyCENon1August2003. CENmembersareboundtocomplywiththeCEN/CENELECInternalRegulationswhichstipulatetheconditionsforgivingthisEurope an Standardthestatusofanationalstandardwithout
9、anyalteration.Uptodatelistsandbibliographicalreferencesconcernings uchnational standardsmaybeobtainedonapplicationtotheManagementCentreortoanyCENmember. ThisEuropeanStandardexistsinthreeofficialversions(English,French,German).Aversioninanyotherlanguagemadebytra nslation undertheresponsibilityofaCENm
10、emberintoitsownlanguageandnotifiedtotheManagementCentrehasthesamestatusasthe official versions. CENmembersarethenationalstandardsbodiesofAustria,Belgium,CzechRepublic,Denmark,Finland,France,Germany,Greece, Hungary,Iceland,Ireland,Italy,Luxembourg,Malta,Netherlands,Norway,Portugal,Slovakia,Spain,Swed
11、en,SwitzerlandandUn ited Kingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPISCHESKOMITEEFRNORMUNG ManagementCentre:ruedeStassart,36B1050Brussels 2003CEN Allrightsofexploitationinanyformandbyanymeansreserved worldwideforCENnationalMembers. Ref.No.EN14119:2003EEN14119:2003(
12、E) 2 Contents Page Foreword. 3 Introduction 4 1 Scope . 5 2 Normativereferences . 5 3 Termsanddefinitions. 5 4 Safety . 5 5 Principle. 6 6 Apparatus 7 7 Reagents 7 8 Testspecimens . 9 9 Preparationoftestspecimens. 10 10 Procedure 11 11 Evaluation 17 12 Expressionofresults . 18 13 Accuracyofmeasureme
13、nts. 18 14 Testreport . 19 AnnexA (informative) Recommendationsfortesting 20 AnnexB (informative) Informationonthetestfungi 21EN14119:2003(E) 3 Foreword Thisdocument(EN14119:2003)hasbeenpreparedbyTechnicalCommitteeCEN/TC248“Textilesand textileproducts”,thesecretariatofwhichisheldbyBSI. ThisEuropeanS
14、tandardshallbegiventhestatusofanationalstandard,eitherbypublicationofanidentical textorbyendorsement,atthelatestbyMarch2004,andconflictingnationalstandardsshallbewithdrawnat thelatestbyMarch2004. AnnexesAandBareinformative. AccordingtotheCEN/CENELECInternalRegulations,thenationalstandardsorganizatio
15、nsofthefollowing countriesareboundtoimplementthisEuropeanStandard:Austria,Belgium,CzechRepublic,Denmark, Finland,France,Germany,Greece,Hungary,Iceland,Ireland,Italy,Luxembourg,Malta,Netherlands, Norway,Portugal,Slovakia,Spain,Sweden,SwitzerlandandtheUnitedKingdom.EN14119:2003(E) 4 Introduction Under
16、certainclimaticandenvironmentalconditionsmicrofungicansettleonandcolonisethesurfaceof textileproductsandcanpossiblydeterioratethem. Thetestsandtestconditionsspecifiedinthisstandardareempiricalandcovermostbutnotallpotential applications. Theactionsofmicrofungiontextilesarequitedifferent: a) Directact
17、ion:deteriorationofthetextileproductwhichserveasanutritivesubstanceforthegrowthofthe microfungi; b) Indirectaction:influenceofmetabolicproductsofthemicrofungi,e.g.discolourationorbiochemical deterioration. Thisstandarddealsalsowiththecombinedactionofthesetwoprocesses. ThisstandardisbasedonapartofISO
18、846:1997“PlasticsEvaluationoftheactionofmicroorganisms“and IEC68210:1988“Basicenvironmentaltestingprocedures,Part2:TestJ,Mouldgrowth“.EN14119:2003(E) 5 1Scope ThisEuropeanStandardspecifiesmethodsfordeterminingtheresistanceoftextilestotheactionof microfungi.Theresultsofthetestsareevaluatedbyvisualexa
19、minationandbymeasuringthephysical propertiesofthetextiles.Thesetestsareapplicabletoalltextileproducts,includingcellulosicormanmade fibretextiles(seeannexA). 2 Normativereferences ThisEuropeanStandardincorporates,bydatedorundatedreference,provisionsfromotherpublications. Thesenormativereferencesareci
20、tedattheappropriateplacesinthetext,andthepublicationsarelisted hereafter.Fordatedreferences,subsequentamendmentstoorrevisionsofanyofthesepublicationsapplyto thisEuropeanStandardonlywhenincorporatedinitbyamendmentorrevision.Forundatedreferencesthe latesteditionofthepublicationreferredtoapplies(includ
21、ingamendments). ENISO139341,TextilesTensilepropertiesoffabricsPart1:Determinationofmaximumforceand elongationatmaximumforceusingthestripmethod(ISO139341:1999). 3 Termsanddefinitions ForthepurposesofthisEuropeanStandardthefollowingtermsanddefinitionsapply. 3.1 biodeterioration changeofchemicalorphysi
22、calpropertiesofmaterialsduetotheactionof(micro)organisms 3.2 fungistaticeffect antimycoticeffectofanantimicrobialtreatmentwhichpreventsagivenmaterialfrombeingovergrownbyfungi undermoistconditions 3.3 biodegradation ultimatedegradation/mineralizationoforganicmatterunderaerobicconditionsintoCO 2 ,wate
23、randmicrobial biomass 3.4 rotting microbiogenousdeteriorationundermoistorwetconditions,incontactwithamicrobiologicalactivesoil 4Safety Handlingofmicroorganismswhicharepotentiallyhazardousrequiresahighdegreeoftechnicalcompetence andcanbesubjecttocurrentnationallegislationandregulations.Onlypersonnelt
24、rainedinmicrobiological techniquesshouldcarryoutsuchtests.Codesofpracticefordisinfection,sterilisationandpersonalhygiene shallbestrictlyobserved. NOTE ItisrecommendedthatworkersshouldconsultappendixA“Dangertopersonnel“ofIEC68210andISO 7218“MicrobiologyGeneralguidanceformicrobiologicalexaminations“.E
25、N14119:2003(E) 6 5Principle 5.1General Thetestsinvolveexposingtestspecimensoftextilestotheactionofselectedteststrainsofmicrofungifor specifiedoragreedperiodsunderspecifiedtemperatureandhumidity. Attheendoftheexposure,thetestspecimensareassessedbeforeand/oraftercleaningbyvisual examinationand/orchang
26、einphysicalproperties,e.g.tensilestrength. Theresultsofthetestspecimensexposedtobiologicalattackarecomparedwithunexposedspecimens. Briefdescriptionsofeachmethodaregivenin5.2to5.5. 5.2 MethodA1:Fungalgrowthtestonincompleteagarmedium Thetestspecimensareexposedonanincompletenutritivemedium(agarmediumwi
27、thoutacarbonsource)to astandardizedmixedsporesuspensionofthetestfungi.Thetestfungicanonlygrowattheexpenseofthe material.Thestandardcontainsfungifortestingcelluloseorcellulosecontainingtextiles.Theinfluenceofthe testfungiisevaluatedbygrowthratingandtensilestrengthevaluation. 5.3 MethodA2:Fungalgrowth
28、testinamoistchamber Thetestspecimensareinoculatedwithastandardizedmixedsporesuspensionandexposedinaneartothe practicetest(moistchambertest)todeterminetheinfluenceofthetestfungi.Testfungiareproposedfor celluloseorcellulosecontainingtextiles.Theinfluenceofthefungiisevaluatedbygrowthratingandbytensile
29、strengthevaluation. 5.4 MethodB1:Fungalgrowthtestonacompleteagarmedium Thetestspecimensareexposedonacompletenutritivemedium(agarmediumwithacarbonsource)toa standardizedmixedsporesuspensionofthetestfungi.Thetestfungicangrowontheagarmediumandon thetextile,ifthetextileisnotprotectedagainstfungalgrowth.
30、Thestandardcontainsfungifortestingcellulose orcellulosecontainingtextiles.Theinfluenceofthetestfungiisevaluatedbygrowthrating(determinationof theantimycoticeffect)andbytensilestrengthevaluation(determinationoftheresistanceofthetextileagainst biodeterioration). 5.5 Antimycoticeffectofatextilematerial
31、 5.5.1 MethodB1:Fungalgrowthtestoncompleteagarmedium;antimycoticeffect See5.4 5.5.2 MethodB2:Antimycoticactivity Thetestspecimensarelaidonaninoculatedcompletenutritivemedium(agarwithcarbonsourceand incorporatedfungalspores(singlecultures).Thetestfunguscangrowinandontheagarnearthetextile specimen,ift
32、hetextileisnotprotectedagainstfungalgrowth.ThestandardtestisusingAspergillusnigerand Chaetomiumglobosumfortesting.Thetestgermspectrumcanbeadapted.Theantimycoticeffectagainstthe teststrainisevaluatedbygrowthrating(determinationoftheantimycoticeffect)andmeasuringofapossibly zonewithoutfungalgrowtharou
33、ndthespecimen. NOTE1 MethodAallowsthedeterminationofthefungalresistanceofcelluloseorcellulosecontainingtextilesorother susceptibletextilesandtheevaluationofanantimicrobialfinishing. NOTE2 MethodBallowsthedeterminationoftheactionofatextilefinishingagainstselectedteststrains.Itallowsthe invitrocompari
34、sonofdifferentfinishings.EN14119:2003(E) 7 5.6 Choiceofpropertiesforassessment Avisualexaminationofthebiologicalattack,thefungalgrowthorformationofaninhibitionzoneagainstthe testfungishouldalwaysbemadeasthefirststageinassessingtheresistanceofa textile. Tensilestrengthevaluationallowsthedetermination
35、oftheresistanceofatextileagainstmicrofungi.Tensile strengthtestsarenecessaryfortestingtheresistanceofcellulosictextiles. Furtherevaluationscanbeagreed.Thechoicedependsontheaimofthetesting. 6Apparatus 6.1 Microbiologicalincubators ,capableofcontrollingtemperatureto(291)C. 6.2 Ovencapableofcontrolling
36、temperatureat(451)Cfordryingthetestspecimens. 6.3 Ambientconditioningtoattainstandardtemperatureandhumidityconditions(20Cand65%relative humidity(r.h.)forconditioningthespecimensfortensilestrengthtests. NOTE Ambientconditions23Cand50%r.h.areoptionalandshouldbeagreedbetweenthepartners. 6.4 Autoclaveca
37、pableofattainingatemperatureandpressureof121Cand2barrespectively. 6.5 Analyticalbalance (graduationof1mg)forpreparingmedia. 6.6 Laboratorycentrifuge. 6.7 Stereoscopicmicroscope (magnification50X). 6.8 Testcontainers: glassorplasticPetridishesofapproximately90mmdiameter. 6.9 Glassorplasticcontainers,
38、e.g.withavolumeofabout1l(height,16cm;diameter,11cm),for examplepreservingjarswithcoversoranotherhumidchamberfacility. 7Reagents 7.1Distilledordeionisedwaterwithaconductivityof1S/cm. forthepreparationofsolutions,nutritive mediaandthetests. 7.2 Microbicidalsolutions forcleaningthetextilebeforeandafter
39、thebiotest,ethanolwatermixtureatamass ratioof70:30. 7.3 TestfungiTeststrains. T hetestfungishallbeobtainedwheneverpossiblefromnationalculture collections.Thestrainstobeusedarelistedintable1andshallbestatedinthetestreport.EN14119:2003(E) 8 Table1Teststrains Name Strain Aspergillusniger vanTieghem ATC
40、C6275 DSM1957 IMI45551 Chaetomiumglobosum Kunze:Fries ATCC6205 DSM1962 IMI45550 Gliocladiumvirens Milleretal. ATCC9645 DSM1963 IMI45553 Paecilomycesvariotii Bainier ATCC18502 DSM1961 IMI40025 Penicilliumpinophilum Thom ATCC36839 DSM1944 IMI114933 Iftherearetechnicalreasons,andbyagreementbetweenthein
41、terestedparties,additionalspeciesmaybe used(seeannexB).Thestrainsusedshallbestatedinthetestreport. 7.4 Stockstrains Thetestfungicanbeculturedintubesonagarslantsofthefollowingcomposition: oatmeal 20 g maltextract 10g agar 20 g distilledordeionizedwater 1000ml Sterilizeat121Cfor15minto20mininanautocla
42、vewithsaturatedwatervapour. Afterincubationat(291)Cwellsporulatingculturesmaybeused.Theyshouldnotbestoredformorethan 4weeksatthistemperature. Becauseofthepossibilityofgeneticandphysiologicalchangesinthetestfungiduringculturingonartificial mediatheintervalsbetweensubculturingshouldbereducedtoaminimum
43、bysuitablemeasures(lyophilised cultures,storageat+4Corinliquidnitrogen). 7.5 Solutionsandmedia 7.5.1 Stockmineralsaltssolution (usesubstancesofanalyticalorequivalentpurity) NaNO 3 2,0g KH 2 PO 4 0,7g K 2 HPO 4 0,3g KCl 0,5g MgSO 4 7H 2 O 0,5 g FeSO 4 7H 2 O0,01 g H 2 O 1000mlEN14119:2003(E) 9 AdjustthepHto6.0to6.5byadding0,01mol/lsterileNaOHsolution. Thefollowingsterilesolutionsareprepared fromthestockmineralsaltssolution. 7.5.2 Mineral saltssolutionwithwettingagent. Addto1lofthestockmineralsaltssolutio
