1、 Dansk standard DS/EN ISO 734-12. udgave2007-01-04Mel af olieholdige fr Bestemmelse af olieindhold Del 1: Ekstraktions-metode med brug af hexan (eller let petroleum) Oilseed meals Determination of oil content Part 1: Extraction method with hexane (or light petroleum) DS-publikationstyper Dansk Stand
2、ard udgiver forskellige publikationstyper. Typen p denne publikation fremgr af forsiden. Der kan vre tale om: Dansk standard standard, der er udarbejdet p nationalt niveau, eller som er baseret p et andet lands nationale standard, eller standard, der er udarbejdet p internationalt og/eller europisk
3、niveau, og som har fet status som dansk standard DS-information publikation, der er udarbejdet p nationalt niveau, og som ikke har opnet status som standard, eller publikation, der er udarbejdet p internationalt og/eller europisk niveau, og som ikke har fet status som standard, fx en teknisk rapport
4、, eller europisk prstandard DS-hndbog samling af standarder, eventuelt suppleret med informativt materiale DS-hfte publikation med informativt materiale Til disse publikationstyper kan endvidere udgives tillg og rettelsesblade DS-publikationsform Publikationstyperne udgives i forskellig form som hen
5、holdsvis fuldtekstpublikation (publikationen er trykt i sin helhed) godkendelsesblad (publikationen leveres i kopi med et trykt DS-omslag) elektronisk (publikationen leveres p et elektronisk medie) DS-betegnelse Alle DS-publikationers betegnelse begynder med DS efterfulgt af et eller flere prfikser
6、og et nr., fx DS 383, DS/EN 5414 osv. Hvis der efter nr. er angivet et A eller Cor, betyder det, enten at det er et tillg eller et rettelsesblad til hovedstandarden, eller at det er indfrt i hovedstandarden. DS-betegnelse angives p forsiden. Overensstemmelse med anden publikation: Overensstemmelse k
7、an enten vre IDT, EQV, NEQ eller MOD IDT: Nr publikationen er identisk med en given publikation. EQV: Nr publikationen teknisk er i overensstemmelse med en given publikation, men prsentationen er ndret. NEQ: Nr publikationen teknisk eller prsentationsmssigt ikke er i overensstemmelse med en given st
8、andard, men udarbejdet p baggrund af denne. MOD: Nr publikationen er modificeret i forhold til en given publikation. DS/EN ISO 734-1 Kbenhavn DS projekt: 51200 ICS: 67.200.20 Frste del af denne publikations betegnelse er: DS/EN ISO, hvilket betyder, at det er en international standard, der har statu
9、s bde som europisk og dansk standard. Denne publikations overensstemmelse er: IDT med: ISO 734-1:2006. IDT med: EN ISO 734-1:2006. DS-publikationen er p engelsk. Denne publikation erstatter: DS/EN ISO 734-1:2000. EUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 734-1November 2006ICS 67.200.20 S
10、upersedes EN ISO 734-1:2000 English VersionOilseed meals - Determination of oil content - Part 1: Extractionmethod with hexane (or light petroleum) (ISO 734-1:2006)Tourteaux de graines olagineuses - Dtermination de lateneur en huile - Partie 1: Mthode par extraction lhexane (ou lther de ptrole) (ISO
11、 734-1:2006)lsamenschrote - Bestimmung des lgehaltes - Teil 1:Extraktionsverfahren mit Hexan (oder Petrolether) (ISO734-1:2006)This European Standard was approved by CEN on 28 October 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for gi
12、ving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versio
13、ns (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cypru
14、s, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT E
15、UROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2006 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 734-1:2006: EEN ISO 734-1:2006 (E) 2 Foreword This document (EN ISO
16、734-1:2006) has been prepared by Technical Committee ISO/TC 34 “Agricultural food products“ in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis“, the secretariat of which is held by AFNOR. This do
17、cument supersedes EN ISO 734-1:2000. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2007, and conflicting national standards shall be withdrawn at the latest by May 2007. According to the CEN
18、/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg,
19、 Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 734-1:2006 has been approved by CEN as EN ISO 734-1:2006 without any modifications. Reference numberISO 734-1:2006(E)ISO 2006INTERNATIONAL STA
20、NDARD ISO734-1Second edition2006-11-01Oilseed meals Determination of oil content Part 1: Extraction method with hexane (or light petroleum) Tourteaux de graines olagineuses Dtermination de la teneur en huile Partie 1: Mthode par extraction lhexane (ou lther de ptrole) ISO 734-1:2006(E) PDF disclaime
21、r This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accep
22、t therein the responsibility of not infringing Adobes licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the fi
23、le; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ISO 2006 All rights
24、 reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs member body in the country of the r
25、equester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org ii ISO 2006 All rights reservedISO 734-1:2006(E) ISO 2006 All rights reserved iiiForeword ISO (the International Organization for Standardization) is
26、a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represent
27、ed on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are dra
28、fted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International St
29、andard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 734-1 was prepared
30、 by Technical Committee ISO/TC 34, Food products, Subcommittee SC 2, Oleaginous seeds and fruits and oilseed meals. This second edition cancels and replaces the first edition (ISO 734-1:1998), Annex A of which has been technically revised. ISO 734 consists of the following parts, under the general t
31、itle Oilseed meals Determination of oil content: Part 1: Extraction method with hexane (or light petroleum) Part 2: Rapid extraction method ISO 734-1:2006(E) iv ISO 2006 All rights reservedIntroduction A method for the determination of the oil content of oilseeds has been specified in ISO 659. It is
32、 therefore necessary to provide for control of oil production by establishing a reference method for the determination of the oil content of oilseed meals in the same way. INTERNATIONAL STANDARD ISO 734-1:2006(E) ISO 2006 All rights reserved 1Oilseed meals Determination of oil content Part 1: Extrac
33、tion method with hexane (or light petroleum) 1 Scope This part of ISO 734 specifies a method for the determination of the hexane extract (or light-petroleum extract), called “oil content”, of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvent
34、s. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 771, Oilseed residu
35、es Determination of moisture and volatile matter content ISO 5502, Oilseed residues Preparation of test samples 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil content all of the substances extracted under the operating conditions specifi
36、ed in this part of ISO 734, and expressed as a mass fraction, in percent, of the product as received NOTE The oil content may also be expressed relative to dry matter. 4 Principle A test portion of the product is extracted in a suitable apparatus, with technical hexane or, failing this, light petrol
37、eum. The solvent is eliminated and the extract obtained is weighed. 5 Reagents Use only reagents of recognized analytical grade, unless otherwise specified. 5.1 Technical hexane, n-hexane or light petroleum, essentially composed of hydrocarbons with six carbon atoms. Less than 5 % shall distil below
38、 50 C and more than 95 % between 50 C and 70 C. For any of these solvents, the residue on complete evaporation shall not exceed 2 mg per 100 ml. ISO 734-1:2006(E) 2 ISO 2006 All rights reserved6 Apparatus Usual laboratory apparatus and, in particular, the following. 6.1 Mechanical grinder, easy to c
39、lean and allowing the meals to be ground, without heating and without appreciable change in moisture, volatile matter and oil content, to obtain particles which pass completely through a sieve of aperture size 1 mm. 6.2 Mechanical microgrinder, of the Dangoumau type1)capable of producing a fineness
40、of grinding of oilseed meals of less than 160 m, with the exception of the “shell” whose particles may reach 400 m. In laboratories where a microgrinder is not available, microgrinding of the ground sample (see 9.4.3) may be replaced by trituration with a pestle and mortar, in the presence of about
41、10 g of sand that has been washed with hydrochloric acid and then calcined. However, grinding in a mortar cannot be applied in the case of multiple analyses because operator fatigue prevents sufficiently efficient grinding of numerous samples, and the extraction of oil from a coarsely ground sample
42、can never be complete. 6.3 Extraction thimble and cotton wool, or filter paper, free from matter soluble in hexane or light petroleum. 6.4 Suitable extraction apparatus, fitted with a flask of capacity 200 ml to 250 ml. NOTE Straight-through extractors, for example the Butt, Smalley, Twisselmann and
43、 Bolton-Williams2)are suitable. The use of other extractors is conditional upon the results of a test on a standard material of known oil content to confirm the suitability of the apparatus. 6.5 Electric heating bath (e.g. sand bath, water bath) or hot plate. 6.6 Electrically heated oven, with therm
44、ostatic control, permitting ventilation or obtaining reduced pressure, capable of being maintained at 103 C + 2 C. 6.7 Desiccator, containing an efficient desiccant. 6.8 Pumice stone, in small particles, previously dried in an oven at 103 C + 2 C and cooled in a dessicator. 6.9 Analytical balance, c
45、apable of weighing to an accuracy of + 0,001 g. 7 Sampling A representative sample should have been sent to the laboratory. It should not have been damaged or changed during transport or storage. Sampling is not part of the method specified in this part of ISO 734. A recommended sampling method is g
46、iven in ISO 5500. 1) The Dangoumau mechanical microgrinder is an example of suitable apparatus available commercially. This information is given for the convenience or users of this part of ISO 734 and does not constitute an endorsement by ISO of this apparatus. 2) The Butt, Smalley, Twisselmann or
47、Bolton-Williams straight-through extractors are examples of suitable apparatus available commercially. This information is given for the convenience of users of this part of ISO 734 and does not constitute an endorsement by ISO of this apparatus. ISO 734-1:2006(E) ISO 2006 All rights reserved 38 Pre
48、paration of test sample 8.1 Prepare the test sample in accordance with ISO 5502. 8.2 If necessary, grind the test sample, in the previously well-cleaned mechanical mill (6.1). First, use about one-twentieth of the sample to complete the cleaning of the mill, and reject these grindings. Then grind th
49、e rest, collect the grindings, mix carefully and carry out the analysis without delay. 9 Procedure 9.1 Number of determinations If it is required to check whether the repeatability (11.2) is met, carry out two single determinations in accordance with 9.2 to 9.4.4. 9.2 Test portion 9.2.1 Weigh, to the nearest 0,001 g, about 10 g of the test sample (8.2). 9.2.2 Transfer this test portion to the extraction thimble (6.3) and
