1、BS EN 46-1:2016Wood preservatives Determination of thepreventive action againstrecently hatched larvae ofHylotrupes bajulus (Linnaeus)Part 1: Application by surface treatment(laboratory method)BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS EN 46-1:2016 BRITISH ST
2、ANDARDNational forewordThis British Standard is the UK implementation of EN 46-1:2016. Itsupersedes BS EN 46-1:2009 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A list of organizations represented on this committee can beobta
3、ined on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2016. Published by BSI StandardsLimited 2016ISBN 978 0 580 90448 6ICS 71.100.50Compliance with
4、a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 30 June 2016.Amendments issued since publicationDate Text affectedBS EN 46-1:2016EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM
5、EN 46-1 June 2016 ICS 71.100.50 Supersedes EN 46-1:2009English Version Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method) Produits de prservation du bois - Dtermin
6、ation de laction prventive contre les larves rcemment closes dHylotrupes bajulus (Linnaeus) - Partie 1: Application par traitement de surface (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1:
7、Anwendung durch Oberflchenverfahren (Laboratoriumsverfahren) This European Standard was approved by CEN on 5 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard with
8、out any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other
9、language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, De
10、nmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITT
11、EE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 46-1:2016 EBS EN 46-1:2016EN
12、 46-1:2016 (E) 2 Contents Page European foreword . 4 Introduction 5 1 Scope 6 2 Normative references 6 3 Terms and definitions . 6 4 Principle . 7 5 Test materials . 7 5.1 Biological material 7 5.2 Products and reagents . 7 5.3 Apparatus . 7 6 Sampling . 8 7 Test specimens . 8 7.1 Species of wood 8
13、7.2 Wood quality . 9 7.3 Provision of test specimens . 9 7.4 Dimensions of test specimens 9 7.5 Number of test specimens 9 8 Procedure 10 8.1 Preparation of the test specimens . 10 8.1.1 Conditioning of the test specimens prior to sealing 10 8.1.2 Sealing of the transverse faces 10 8.1.3 Treatment o
14、f the test specimens 10 8.1.4 Drying and conditioning of the test specimens after treatment . 12 8.2 Exposure of the test specimens to the insects . 12 8.3 Conditions and duration of the test . 13 8.4 Examination of the test specimens 13 8.4.1 Examination . 13 8.4.2 Validity of the test 14 9 Express
15、ion of results . 14 9.1 Evaluation of attack . 14 9.2 Toxic values 14 10 Test report 15 Annex A (informative) Example of a test report 17 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) 19 B.1 General . 19 B.2 Obtaining parent beetles . 19 B.3 Mating . 19 B.4 Egg-Iaying 1
16、9 BS EN 46-1:2016EN 46-1:2016 (E) 3 B.5 Hatching of eggs 20 B.6 Larval development . 20 B.7 Enemies and parasites 21 Annex C (informative) Differentiation of heartwood and sapwood in Pinus species . 22 C.1 Principle . 22 C.2 Reagents . 22 C.3 Apparatus 22 C.4 Procedure 22 Annex D (informative) Envir
17、onmental, health and safety precautions within chemical/biological laboratory . 23 Annex E (normative) Slow acting and deferred effect formulations Extending the test duration . 24 Bibliography . 26 BS EN 46-1:2016EN 46-1:2016 (E) 4 European foreword This document (EN 46-1:2016) has been prepared by
18、 Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflicti
19、ng national standards shall be withdrawn at the latest by December 2016. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document
20、 supersedes EN 46-1:2009. Significant technical differences between this document and EN 46-1:2009 are as follows: a) introduction of new harmonized specifications for wood quality; b) option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is wat
21、er of drinking quality; c) determination of validity of the test by using an X-ray apparatus added in 8.4.2. The standard EN 46 is composed of two parts: EN 46-1, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Applic
22、ation by surface treatment (laboratory method) EN 46-2, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory method) EN 46 consists of two parts to enable preventive action of wood preservatives
23、, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidial effect of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. Acco
24、rding to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany
25、, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. BS EN 46-1:2016EN 46-1:2016 (E) 5 Introduction This European Standard describes a laborator
26、y method of testing which gives a basis for the assessment of the preventive action of a wood preservative, when applied as a surface treatment, against recently hatched larvae of Hylotrupes bajulus, whereas the method for determining the toxic values against Hylotrupes bajulus (EN 47) provides a me
27、ans of checking whether a preservative prevents attack by these larvae and prevents their survival within totally impregnated wood. This method makes it possible to determine whether recently hatched larvae are capable of boring through the treated surface of a susceptible wood species and of surviv
28、ing in the untreated part of the test specimen. For this purpose, the procedure seeks to reproduce normal egg-laying conditions existing in cracks in wood, which provide the principal egg-laying sites. It takes account of the fact that, if larvae pass through the treated surface, they will then tunn
29、el in the direction of the least protected regions of the wood. This laboratory method provides one criterion by which the value of a preservative can be assessed. In making this assessment, the methods by which the preservative may be applied should be taken into account. This test is of particular
30、 interest when applied to test specimens which have been subjected to an ageing procedure. It is further recommended that results from this test should be supplemented by those from other appropriate tests and, above all, by practical experience. When products which are very active at low concentrat
31、ions are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, ext
32、raction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). BS EN 46-1:2016EN 46-1:2016 (E) 6 1 Scope This European Standard specifies a method for the determination of the preventive action of a wood preservative
33、against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory
34、 by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedur
35、es. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any a
36、mendments) applies. EN 73, Wood preservatives Accelerated ageing of treated wood prior to biological testing Evaporative ageing procedure EN 84, Wood preservatives Accelerated ageing of treated wood prior to biological testing Leaching procedure EN ISO 3696, Water for analytical laboratory use Speci
37、fication and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume bein
38、g sampled SOURCE: EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. BS EN 46-1:2016EN 46-1:2016 (E) 7 4 Principle Depending on the test being carried out either: on a set
39、 of test specimens of a susceptible wood species that is surface treated with a solution of the preservative; or if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are surface treated with a series of solutions in which the concentration of pre
40、servative is ranged in a given progression. The treated test specimens are exposed to recently hatched larvae of Hylotrupes bajulus. The resulting attack is observed and compared with those in untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a
41、concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae, within three days of hatching. 5.1.2 Source of larvae. Obtain the larvae
42、from cultures reared, e.g. as described in Annex B. 5.1.3 Provision of larvae. Collect larvae from eggs laid by different females. 5.1.4 Choice of larvae. Use a mixed batch of these larvae for the test. Use ten larvae per treated test specimen or control test specimen. 5.2 Products and reagents 5.2.
43、1 Paraffin wax, for fixing the glass plate and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatin, for sealing the end faces
44、of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the
45、 post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 2) C and at a relative humidity of (70 5) %. 5.3.2 Conditioning chambe
46、r, well ventilated and controlled at (20 2) C and at a relative humidity of (65 5) %. BS EN 46-1:2016EN 46-1:2016 (E) 8 The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.
47、2). 5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.4 Testing chamber, ventilated
48、 and air conditioned, controlled at (22 2) C and at a relative humidity of (70 5) %. 5.3.5 Treatment vessels of a material that does not react with the preservative under test, for example of glass for organic products and of polyethylene for salts containing fluorine. 5.3.6 Weights, to provide ball
49、ast for the test specimens. The weights shall not react with any materials with which they come into contact during the test. 5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.8 Glass plates, (48 1) mm long and (25 1) mm wide, intended to provide a lateral slit on the test specimens. 5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g. 5.3.10 Protective gloves 5.3
