1、BSI Standards PublicationBS EN 49-2:2015Wood preservatives Determination of theprotective effectiveness againstAnobium punctatum (DeGeer) by egg-laying and larvalsurvivalPart 2: Application by impregnation(Laboratory method)BS EN 49-2:2015 BRITISH STANDARDNational forewordThis British Standard is th
2、e UK implementation of EN 49-2:2015. Itsupersedes BS EN 49-2:2005 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A list of organizations represented on this committee can beobtained on request to its secretary.This publication
3、does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2015. Published by BSI StandardsLimited 2015ISBN 978 0 580 88434 4ICS 71.100.50Compliance with a British Standard cannot confer immunity fromlega
4、l obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 August 2015.Amendments issued since publicationDate Text affectedBS EN 49-2:2015EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 49-2 August 2015 ICS 71.100.50 Supersedes EN
5、49-2:2005English Version Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method) Produits de prservation du bois - Dtermination de lefficacit protectrice vis vis
6、 de Anobium punctatum (De Geer) par lobservation de la ponte et de la survie des larves - Partie 2 : Application par imprgnation (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Lar
7、ven -Teil 2: Anwendung durch Volltrnkung (Laboratoriumsverfahren) This European Standard was approved by CEN on 16 July 2015. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard w
8、ithout any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any oth
9、er language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic,
10、 Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMM
11、ITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 49-2:2015 EBS EN 49-2:201
12、5EN 49-2:2015 (E) 2 Contents Page European foreword .3 Introduction .4 1 Scope 5 2 Normative reference 5 3 Terms and definitions .5 4 Principle 5 5 Test materials .6 5.1 Biological material .6 5.2 Products and reagents 6 5.3 Apparatus .6 6 Sampling .7 7 Test specimens 8 7.1 Species of wood.8 7.2 Woo
13、d quality 8 7.3 Provision of test specimens .8 7.4 Dimensions of test specimens .8 7.5 Number of test specimens 8 8 Procedure .9 8.1 Preparation of the test specimens .9 8.1.1 Conditioning of test specimens before treatment 9 8.1.2 Treatment of the test specimens 9 8.1.3 Drying and conditioning of t
14、he test specimens after treatment 10 8.2 Exposure of the test specimens to the insects . 11 8.3 Conditions and duration of the test 11 8.4 Examination of the test specimens . 11 9 Validity of test . 12 10 Expression of results . 12 10.1 Assessment of the protective effectiveness 12 10.2 Toxic values
15、 12 11 Test report . 12 Annex A (informative) Example of a test report 14 Annex B (informative) Identification of sex of test insects (Anobium punctatum) . 16 Annex C (informative) Culturing technique for Anobium punctatum . 17 Annex D (informative) Environmental, health and safety precautions withi
16、n chemical/biological laboratory 20 Bibliography . 21 BS EN 49-2:2015EN 49-2:2015 (E) 3 European foreword This document (EN 49-2:2015) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard sh
17、all be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by February 2016 and conflicting national standards shall be withdrawn at the latest by February 2016. Attention is drawn to the possibility that some of the elements of this d
18、ocument may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 49-2:2005. Significant technical differences between this document and EN 49-2:2005 are as follows: a) generalization of material f
19、or preparing the egg-laying zones; b) introduction of new harmonized specifications for wood quality. EN 49, Wood preservatives Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival, consists of two parts: Part 1: Application by surface t
20、reatment (Laboratory method); Part 2: Application by impregnation (Laboratory method). EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to be applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation. According to
21、the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
22、 Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. BS EN 49-2:2015EN 49-2:2015 (E) 4 Introduction This document describes a laboratory method of testin
23、g which gives a basis for assessment of the effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the concentration at which the product prevents the development of infestation from egg laying. The method simulates conditions which can occur in practice on t
24、imber which has been treated some time previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservativ
25、e may be applied should be taken into account. It is further recommended that results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very impo
26、rtant to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, condition
27、ing chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). BS EN 49-2:2015EN 49-2:2015 (E) 5 1 Scope This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative ag
28、ainst Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laborato
29、ry by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, and water-soluble materials, for example salts. NOTE This method can be used in conjunction with an ageing procedure, for example EN 73. 2 Normative refere
30、nce The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN
31、ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical characteristics identical to the volumetric
32、 average characteristics of the total volume being sampled 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) 4 Principle The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid, the numbers of e
33、ggs hatched, and the numbers of the surviving larvae are compared with those in untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent tre
34、ated control test specimens. Depending on the test being carried out either on a set of test specimens of a susceptible wood species that is impregnated with a solution of the preservative, or BS EN 49-2:2015EN 49-2:2015 (E) 6 if toxic values are to be determined, on several sets of test specimens o
35、f a susceptible wood species that are impregnated with a series of solutions in which the concentration of preservative is ranged in a given progression. 5 Test materials 5.1 Biological material Anobium punctatum (De Geer) Adult males and females in good condition. Adults to be used in the test shal
36、l be collected daily from naturally infested wood or laboratory culture (see Annex C). Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.2.2 and C.6) and then checked to ensure that they are undamaged, active, and free from any infestation by mites.
37、Determine the sex (see Annex B) of the collected and checked adults and place the males and females in separate containers. NOTE The proportion of males and females varies during the emergence period. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the end sections of test specimens. NOTE
38、Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and insoluble in the product under test. NOTE Sodium carboxymethyl cellulose, food grade, has been found to be suita
39、ble. 5.2.3 Xylene, technical grade, mixed isomers. 5.2.4 Water, complying with grade 3 of EN ISO 3696. 5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a to
40、xic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.2.6 Filter paper, ordinary quality, medium-fast grade. 5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the egg-laying zones. NOTE Cotton, linen
41、and polyamide-gauze have been proven suitable. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (21 2) C, and at relative humidity (80 5) %. 5.3.2 Conditioning chamber, well ventilated, controlled at (20 2) C and at relative humidity (65 5) %. BS EN 49-2:2015EN 49-2:2015 (E
42、) 7 The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Treatment vessel(s), of a material that does not react with the preservative under test, for example of gl
43、ass for organic products and of polyethylene for salts containing fluorine. 5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of o
44、perators to solvents or their vapours. 5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1). 5.3.6 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-fitting cover and containing supports that will give minimum cont
45、act with treated test specimens to be placed on them. The vessels and supports shall be of a material that does not react with the preservative under test, for example glass for organic compounds and polyethylene for products containing fluorine. 5.3.7 Vacuum vessel(s), fitted with stopcocks, capabl
46、e of receiving the treatment vessels (5.3.3). 5.3.8 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa1). 5.3.9 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test.
47、 5.3.10 Safety equipment and protective clothing and gloves, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.11 Test containers, suitable for holding the test specimens and of material resistant to the solvents used, and fitted with perforated covers
48、to provide a good exchange of air. NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable. 5.3.12 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g. 5.3.13 X-ray apparatus, (optional) with tungsten-target and beryllium
49、 window, with voltage and current continuously variable in the ranges: voltage: 10 kV to 50 kV; current: 0 mA to 15 mA. 6 Sampling The sample of preservative shall be representative of the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier. For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used. 1) 100 Pa = 1 mbar. BS EN 49-2:2015EN 49-2:2015 (E) 8 7 Test specimens 7.1 Species of wood The reference species is European
