1、BSI Standards PublicationBS EN 839:2014Wood preservatives Determination of theprotective effectivenessagainst wood destroyingbasidiomycetes Applicationby surface treatmentBS EN 839:2014 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN 839:2014. Itsupersedes DD CE
2、N/TS 839:2008 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisi
3、ons of a contract. Users are responsible for its correctapplication. The British Standards Institution 2014. Published by BSI StandardsLimited 2014ISBN 978 0 580 81699 4ICS 71.100.50Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published un
4、der the authority of theStandards Policy and Strategy Committee on 30 November 2014.Amendments issued since publicationDate Text affectedBS EN 839:2014EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 839 November 2014 ICS 71.100.50 Supersedes CEN/TS 839:2008English Version Wood preservatives - D
5、etermination of the protective effectiveness against wood destroying basidiomycetes - Application by surface treatment Produits de prservation du bois - Dtermination de lefficacit protectrice vis-vis des champignons basidiomyctes lignivores - Application par traitement de surface Holzschutzmittel -
6、Bestimmung der vorbeugenden Wirksamkeit gegen Holz zerstrende Basidiomyceten - Anwendung mit Oberflchenverfahren This European Standard was approved by CEN on 30 August 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this Euro
7、pean Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official version
8、s (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Be
9、lgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Swit
10、zerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN nation
11、al Members. Ref. No. EN 839:2014 EBS EN 839:2014EN 839:2014 (E) 2 Contents Page Foreword 4 Introduction .5 1 Scope 6 2 Normative references 6 3 Terms and definitions .6 4 Principle 7 5 Test materials and apparatus .7 5.1 Biological material .7 5.2 Products and reagents 8 5.3 Apparatus .9 6 Sampling
12、of the preservative 9 7 Test specimens . 10 7.1 Species of wood 10 7.2 Wood quality . 10 7.3 Provision of the test specimens 10 7.4 Dimensions and density of test specimens . 10 7.5 Number and distribution of test specimens 11 8 Procedure 11 8.1 Preparation of the untreated test specimens 11 8.2 Pre
13、paration of the treated test specimens . 12 8.3 Exposure to fungi . 13 8.4 Culture conditions and duration of test . 13 8.5 Assessment of test . 13 9 Statement of results . 15 10 Test report . 15 Annex A (informative) Test fungi 17 A.1 General information on maintenance and acquisition of test strai
14、ns . 17 A.2 Maintenance and treatment of test fungi . 17 A.3 Information regarding obligatory fungi 18 Annex B (normative) Methods of sterilization . 20 B.1 Ionizing radiation 20 B.2 Epoxyethane-based sterilant . 20 B.3 Epoxypropane-based sterilant 21 B.4 Steam . 21 Annex C (informative) Examination
15、 of colonisation . 22 C.1 General . 22 C.2 Procedure 22 C.3 Assessment of test . 22 C.4 Validity of results 23 BS EN 839:2014EN 839:2014 (E) 3 C.5 Statement of results 23 Annex D (informative) Example of a test report . 25 Annex E (informative) Environmental, health and safety precautions within che
16、mical/biological laboratory . 28 Bibliography 29 BS EN 839:2014EN 839:2014 (E) 4 Foreword This document (EN 839:2014) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the
17、status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2015 and conflicting national standards shall be withdrawn at the latest by May 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject
18、of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes CEN/TS 839:2008. In comparison with the previous version of the document, EN 839:2014 has been revised editorially. This document includes annexes; Annex A, Anne
19、x C, Annex D and Annex E are informative and Annex B is normative. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, E
20、stonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. BS EN 839:2014EN 839
21、:2014 (E) 5 Introduction This European Standard specifies a laboratory method of test which gives a basis for assessing the effectiveness of a wood preservative, when applied as a surface treatment, against wood destroying basidiomycetes. It tests whether the applied treatment is able to prevent the
22、 penetration of the fungi into the untreated interior of the test specimens under the conditions of test. This laboratory method provides one criterion by which the effectiveness of a product can be assessed. In making this assessment, the methods by which the preservative may be applied should be t
23、aken into account. It is also recommended that results from this test should be supplemented by those from other relevant tests and above all by practical experience. The procedures described in this European Standard method are intended to be carried out by suitably trained and/or supervised specia
24、lists. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel. Also see Annex E for environmental, health and safety precautions. BS EN 839:2014EN 839:2014 (E) 6 1 Scope This European Standard
25、 specifies a method of test for the determination of the protective effectiveness of a wood preservative, applied to the surface of the wood, against wood destroying basidiomycetes cultured on an agar medium. The method is applicable to all products which are to be applied by superficial application
26、 processes. This includes: organic solvent-based wood preservatives; or organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates; or water-soluble products; or chemicals which are being studied as active ingredients for application by superfici
27、al processes. This method may be used in conjunction with an ageing procedure, for example EN 73. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest
28、 edition of the referenced document (including any amendments) applies. EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample
29、having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled SOURCE: EN 1001-2, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) SOURCE: EN 1001-2, 4.83, modif
30、ied 3.3 superficial application process process which does not include particular features or procedures intended to overcome the natural resistance of wood to penetration of a wood preservative in its ready to use form SOURCE: EN 1001-2, 4.82 BS EN 839:2014EN 839:2014 (E) 7 4 Principle Several seri
31、es of test specimens of a susceptible wood species are end-sealed with a material to prevent penetration of the wood preservative under test into the end grain of the test specimens. The end-sealed test specimens are treated with the wood preservative under test using the process and application rat
32、e specified by the supplier. NOTE Suitable application methods are brushing, pipetting and dipping. The treated test specimens are exposed to attack by basidiomycetes in pure culture. The performance of the test product is assessed in terms of its ability to prevent decay as determined by the maximu
33、m acceptable loss in mass and the absence of visible decay of the surface and the untreated interior. 5 Test materials and apparatus 5.1 Biological material The test fungi to be used are as follows: 5.1.1 Obligatory fungus in all cases Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15) o
34、n softwood. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %. 5.1.2 Obligatory fungus for particular hazards Coriolus versicolor (Linnaeus) Qulet (CTB 863A) on hardwood and/or on softwood as appropriate. Loss in mass of beech in 16 weeks: a mass fraction of minimum 20
35、%. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 15 %. 5.1.3 Two species to be used compulsorily on the basis of the nature of the test product For all products except creosote-type products: Poria placenta (Fries) Cooke sensu J. Eriksson (FPRL 280) on softwood. Loss in
36、mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %; Gloeophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109) on softwood. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %. For creosotes and similar products: Lentinus lepideus Fries ex Fries (
37、BAM Ebw. 20) on softwood. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %; Lentinus cyathiformis (Schaeffer ex Fries) Bresadola (CTB 67-02B) on hardwood. Loss in mass of beech in 16 weeks: a mass fraction of minimum 20 %. BS EN 839:2014EN 839:2014 (E) 8 5.1.4 Optional
38、 fungi For specific regional uses or conditions, it is also possible to select other fungi on an optional basis. When optional fungi are used, information similar to that given in Annex A for the obligatory fungi should be included in the test report. 5.1.5 Maintenance of strains The strains shall b
39、e maintained and treated (frequency of subculturing, alternation of culture media, etc.) in accordance with the instructions of their laboratory of origin (see A.2). The parent strain shall be maintained in the laboratory of its origin so as to conserve and to ensure its vigour. If tests are not und
40、ertaken regularly or if a strain shows signs of degeneration a new standard culture of the strain should be obtained from the laboratory of its origin for each test (see A.2). When new strains are received, the virulence shall be tested to ensure the strain can achieve the minimum loss in mass (see
41、5.1.1, 5.1.2 and 5.1.3). 5.2 Products and reagents 5.2.1 Culture medium The culture medium is a malt agar medium with the following composition: malt extract: in concentrated form: (50 0,5) g; in powder form: (40 0,5) g; agar causing no inhibition of growth of fungi: (20 0,5) g to (30 0,5) g; water
42、conforming to grade 3 of EN ISO 3696. quantity to make up to 1 000 ml. Prepare this medium by warming the mixture in a boiling water bath or steam bath, stirring until completely dissolved. Place in each culture vessel (5.3.1) a sufficient quantity of the medium to provide a minimum depth of 3 mm to
43、 4 mm when in its in-use position. Close the vessels as specified in 5.3.1 and sterilize in an autoclave at 121 C for 20 min. Let the vessels cool in their in-use position. 5.2.2 Solvents and diluents For water soluble or water dispersible preservatives: water conforming to grade 3 of EN ISO 3696. F
44、or preservatives to be diluted or dissolved in an organic solvent: suitably volatile liquids that leave no residue in the wood that would have a toxic effect on the fungi at the end of the post-treatment conditioning period. BS EN 839:2014EN 839:2014 (E) 9 NOTE Toluene and xylene of recognized analy
45、tical grade have been found suitable. 5.2.3 Fumigant (if necessary) Xylene technical grade. 5.2.4 End-seal compound A material resistant to the penetration of the wood preservative under test and the test fungi, or separate materials for each, and without any fungistatic or fungicidal activity withi
46、n the test specimen. NOTE Three brush coats of a 2-component epoxy lacquer, with drying between each application, have been found to be suitable. 5.3 Apparatus 5.3.1 Culture vessels, Kolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml, providing a flat surface area of bet
47、ween 85 cm2and 120 cm2for the medium. NOTE 1 Examples of suitable vessels are given in EN 113. NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with leak proof lids, the centres of which are pierced with a round hole of up to 15 mm diameter
48、and plugged with a wad of cotton wool. 5.3.2 Drying oven, capable of being controlled at (103 2) C. 5.3.3 Desiccators, with efficient desiccant (silica gel for example). 5.3.4 Conditioning chamber, well ventilated and controlled at (20 2) C and (65 5) % relative humidity. 5.3.5 Drying supports, whic
49、h will give a minimum contact with the treated test specimens. The supports shall be of a material that does not react with the test solvent or test wood preservative, for example glass for organic products. 5.3.6 Culture chamber, (incubator or room), dark and controlled at (22 2) C and (70 5) % relative humidity. 5.3.7 Test specimen supports, made of glass, stainless steel or any other inert material, that is to say, with no risk of having any effect on the culture medium, the fungus, the wood or the tes
