1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN ISO 7827:2012Water quality Evaluation ofthe “ready“, “ultimate“ aerobicbiodegradability of organiccompounds in an aqueousmedium Method by analysisof dissolved organic carbo
2、n(DOC) (ISO 7827:2010)BS EN ISO 7827:2012 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN ISO 7827:2012.It is identical to ISO 7827:2010. It supersedes BS EN ISO 7827:1996which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommit
3、tee EH/3/5, Biological Methods.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution
4、2013. Published by BSI StandardsLimited 2013ISBN 978 0 580 65910 2ICS 13.060.70Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 January 2013.Amendments issued si
5、nce publicationDate Text affectedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 7827 December 2012 ICS 13.060.70 Supersedes EN ISO 7827:1995English Version Water quality - Evaluation of the “ready“, “ultimate“ aerobic biodegradability of organic compounds in an aqueous medium -Method by an
6、alysis of dissolved organic carbon (DOC) (ISO 7827:2010) Qualit de leau - valuation de la biodgradabilit arobie “facile“, “ultime“ des composs organiques en milieu aqueux - Mthode par analyse du carbone organique dissous (COD) (ISO 7827:2010) Wasserbeschaffenheit - Bestimmung der leichten, vollstndi
7、gen aeroben biologischen Abbaubarkeit organischer Stoffe in einem wssrigen Medium - Verfahren mittels Analyse des gelsten organischen Kohlenstoffs (DOC) (ISO 7827:2010) This European Standard was approved by CEN on 8 December 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regula
8、tions which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member
9、. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN
10、 members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, P
11、ortugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and b
12、y any means reserved worldwide for CEN national Members. Ref. No. EN ISO 7827:2012: EBS EN ISO 7827:2012EN ISO 7827:2012 (E) 3 Foreword The text of ISO 7827:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has be
13、en taken over as EN ISO 7827:2012 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2013, and conflic
14、ting national standards shall be withdrawn at the latest by June 2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document s
15、upersedes EN ISO 7827:1995. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republ
16、ic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 7827:2010 has been appro
17、ved by CEN as a EN ISO 7827:2012 without any modification. BS EN ISO 7827:2012ISO 7827:2010(E) ISO 2010 All rights reserved iiiContents Page Foreword iv 1 Scope1 2 Normative references1 3 Terms and definitions .2 4 Principle .3 5 Test environment.3 6 Reagents 3 7 Apparatus.4 8 Procedure.5 9 Calculat
18、ion and expression of results 7 10 Validity of the test8 11 Test report9 Annex A (informative) Typical degradation curve .10 Annex B (informative) Interpretation of results .11 Bibliography13 BS EN ISO 7827:2012ISO 7827:2010(E) iv ISO 2010 All rights reservedForeword ISO (the International Organizat
19、ion for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established h
20、as the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Int
21、ernational Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Public
22、ation as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent ri
23、ghts. ISO 7827 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. This third edition cancels and replaces the second edition (ISO 7827:1994), which has been technically revised. BS EN ISO 7827:2012INTERNATIONAL STANDARD ISO 7827:2010(E) ISO 2010 All
24、 rights reserved 1Water quality Evaluation of the “ready”, “ultimate” aerobic biodegradability of organic compounds in an aqueous medium Method by analysis of dissolved organic carbon (DOC) WARNING Persons using this International Standard should be familiar with normal laboratory practice. This sta
25、ndard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests c
26、onducted according to this International Standard be carried out by suitably trained staff. SAFETY PRECAUTIONS Activated sludge and sewage contain potentially pathogenic organisms. Therefore take appropriate precautions when handling them. Handle toxic test compounds and those whose properties are u
27、nknown with care. 1 Scope This International Standard specifies a method for the evaluation of the “ready” and “ultimate” biodegradability of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, this International Standard also gives specific definitions f
28、or the terms “ready” and “ultimate”. The method applies to organic compounds which are: a) soluble at the concentration used under the conditions of the test dissolved organic carbon (DOC) concentrations of 10 mg/l to 40 mg/l; b) non-volatile or having a negligible vapour pressure under the conditio
29、ns of the test; c) not significantly adsorbable on glass and activated sludge; d) not inhibitory to the test microorganisms at the concentration chosen for the test. The method is not suitable for waste waters, as they usually contain significant amounts of water-insoluble organic carbon, which is n
30、ot included in DOC measurements. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) ap
31、plies. ISO 8245, Water quality Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC) ISO 9408, Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer B
32、S EN ISO 7827:2012ISO 7827:2010(E) 2 ISO 2010 All rights reservedISO 9439, Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium Carbon dioxide evolution test 3 Terms and definitions For the purposes of this document, the following terms and definitions
33、 apply. 3.1 degradation time t2time from the end of the lag time, t1, until the time that about 90 % of the maximum level of biodegradation has been reached NOTE Degradation time is expressed in days. 3.2 inherent biodegradation level of biodegradation achieved which indicates the test compound is u
34、nlikely to be persistent in the environment NOTE See Annex B. 3.3 lag time t1time from the start of the test until 10 % biodegradation has been reached NOTE Lag time is expressed in days. 3.4 maximum level of biodegradation degree of biodegradation of a chemical compound or organic matter in a test
35、above which no further biodegradation takes place during the test 3.5 primary biodegradation structural change (transformation) of a chemical compound by microorganisms resulting in the loss of a specific property of that compound 3.6 “ready” biodegradation level of biodegradation achieved under def
36、ined conditions which indicates the test compound is considered likely to degrade rapidly and completely under aerobic aquatic environmental conditions NOTE See Annex B. 3.7 suspended solids activated sludge solid material within activated sludge with a particle diameter of 45 m NOTE The concentrati
37、on of suspended solids is obtained by filtration or centrifugation of a known volume of sludge under specified conditions, drying at 105 C, and correcting for the volume of sample. The concentration of suspended solids is expressed in milligrams per litre. 3.8 “ultimate” biodegradation breakdown of
38、a chemical compound or organic matter by microorganisms to carbon dioxide, water and mineral salts of any other elements present (mineralization), and the production of new biomass BS EN ISO 7827:2012ISO 7827:2010(E) ISO 2010 All rights reserved 34 Principle The biodegradation of organic compounds b
39、y aerobic microorganisms in a mineral medium is determined by measurement of the DOC concentration. The organic compound is the sole source of carbon in the medium. The concentration of the compound used is such that the initial DOC in the medium is between 10 mg/l and 40 mg/l. If necessary, concent
40、rations greater than 40 mg/l may be used. The test solution is aerated in the dark or diffuse light at 22 C 2 C. Biodegradation is monitored by measurement of the DOC at the start (day 0), at the end of the test (day 28 or longer if necessary), and at a minimum of three intermediate time intervals.
41、The percentage removal of DOC is calculated at each time interval, and the biodegradability of the organic compound based on these data. Specific analysis can give additional information on primary biodegradation. The test is not suitable for compounds which are inhibitory at the concentration used
42、in the test. Inhibitory effects can be determined as specified in 8.3 or by using any other method for determining the inhibitory effect on bacteria of a substance (e.g. ISO 81921). The conditions specified in this International Standard do not necessarily correspond to the optimal conditions allowi
43、ng the maximum degree of biodegradation to occur. Tests for ready biodegradability have very stringent conditions and a substance which passes these is considered likely to be rapidly and completely degraded in any aerobic aquatic environmental compartment, especially in waste water treatment plants
44、. For alternative biodegradation methods, see ISO/TR 154626. See Annex B for information on interpretation of results. 5 Test environment Incubation shall take place in the dark or in diffused light in an enclosure which is maintained at 22 C 2 C and which is free from vapours that are toxic to micr
45、oorganisms. 6 Reagents Use only reagents of recognized analytical grade (where applicable). 6.1 Water, distilled or demineralized, containing less than 10 % of the initial DOC content introduced by the compound to be tested to maintain acceptable precision. 6.2 Test medium 6.2.1 Composition 6.2.1.1
46、Solution A Anhydrous potassium dihydrogenphosphate, KH2PO48,5 g Anhydrous dipotassium hydrogenphosphate, K2HPO421,75 g Disodium hydrogenphosphate dihydrate, Na2HPO42H2O 33,4 g Ammonium chloride, NH4Cl 0,5 Water (6.1), quantity necessary to make up to 1 000 ml Measure the pH value of the solution, wh
47、ich should be 7,4 0,2. If it is not, then prepare a new solution. BS EN ISO 7827:2012ISO 7827:2010(E) 4 ISO 2010 All rights reserved6.2.1.2 Solution B Dissolve 22,5 g of magnesium sulfate heptahydrate (MgSO47H2O) in water (6.1) and make up to 1 000 ml. 6.2.1.3 Solution C Dissolve 27,5 g of anhydrous
48、 calcium chloride (CaCl2) in water (6.1) and make up to 1 000 ml. 6.2.1.4 Solution D Dissolve 0,25 g of iron(III) chloride hexahydrate (FeCl36H2O) in water (6.1) and make up to 1 000 ml. Prepare the solution freshly just before use. The need to prepare this solution just before use can be avoided if
49、 a drop of concentrated hydrochloric acid (HCI) or 0,4 g/l of ethylenediaminetetraacetic acid (EDTA) is added. 6.2.2 Preparation For 1 l of test medium, add to about 500 ml of water (6.1): a) 1 ml of each of solutions B, C, and D; b) 10 ml of solution A. Make up to 1 000 ml with water (6.1). Solution A is added last, to avoid precipitation of salts. Prepare the test medium freshly before use. Solutions A to C can be stored for up to 6 months in the dark at room temperature and Solution D) (with preservat
