1、BRITISH STANDARDBS EN ISO 8655-7:2005Piston-operated volumetric apparatus Part 7: Non-gravimetric methods for the assessment of equipment performanceICS 17.060g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g
2、55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58Incorporating CorrigendumDecember 2008National forewordThis British Standard is the UK implementation of EN ISO 8655-7:2005. It is identical with ISO 8655-7:2005, incorporating corrigendum December 2008.The start and finish of text introduce
3、d or altered by corrigendum is indicated in the text by tags. Text altered by ISO corrigendum December 2008 is indicated in the text by .The UK participation in its preparation was entrusted by Technical Committee LBI/36, Laboratory glassware and related apparatus, to Subcommittee LBI/36/1, General
4、and volumetric, glass.A list of organizations represented on this subcommittee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.Compliance with a British Standard ca
5、nnot confer immunity from legal obligations.BS EN ISO 8655-7:2005This British Standard, having been prepared under the direction of the Materials and Chemicals Sector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 22 October 2002 BS
6、I 2009ISBN 978 0 580 66194 5Amendments/corrigenda issued since publication Date Comments 30 June 2009 Implementation of ISO corrigendum December 2008EUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 8655-7September 2005ICS 17.060English VersionPiston-operated volumetric apparatus - Part 7: Non-g
7、ravimetricmethods for the assessment of equipment performance (ISO8655-7:2005)Appareils volumtriques piston - Partie 7: Mthodes nongravimtriques pour lestimation de la performancedquipement (ISO 8655-7:2005)Volumenmessgerte mit Hubkolben - Teil 7: Nicht-gravimetrische Prfverfahren zur Bewertung derG
8、erteeigenschaften (ISO 8655-7:2005)This European Standard was approved by CEN on 13 August 2005.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-da
9、te lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the re
10、sponsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Irela
11、nd, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Bru
12、ssels 2005 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 8655-7:2005: EBS EN ISO 8655-7:2005Foreword This document (EN ISO 8655-7:2005) has been prepared by Technical Committee ISO/TC 48 “Laboratory glassware and related appara
13、tus“ in collaboration with Technical Committee CEN/TC 332 “Laboratory equipment“, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by March 2006, and conflicti
14、ng national standards shall be withdrawn at the latest by March 2006. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland,
15、 France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 8655-7:2005 has been approved by CEN as EN ISO 8655-7:2005 wi
16、thout any modifications. BS EN ISO 8655-7:2005Reference numberISO 8655-7:2005(E)INTERNATIONAL STANDARD ISO8655-7First edition2005-09-01Piston-operated volumetric apparatus Part 7: Non-gravimetric methods for the assessment of equipment performance Appareils volumtriques piston Partie 7: Mthodes non
17、gravimtriques pour lestimation de la performance dquipement BS EN ISO 8655-7:2005ii BS EN ISO 8655-7:2005iiiContents Page Foreword iv Introduction v 1 Scope . 1 2 Normative references . 1 3 Terms and definitions. 2 4 Principle. 2 4.1 Photometric method. 2 4.2 Titrimetric method 2 5 Reagents 3 5.1 Re
18、agents for photometric method 3 5.2 Reagents for titrimetric method 3 6 Apparatus 4 6.1 Photometric method. 4 6.2 Titrimetric method 4 7 Test conditions . 5 8 Procedure 5 8.1 General. 5 8.2 Photometric method. 5 8.3 Titrimetric method 5 9 Calculation. 6 10 Test report . 6 Annex A (informative) Examp
19、le 1 for photometric test method 7 Annex B (informative) Example 2 for photometric test method 13 Annex C (informative) Example for titration test method. 17 Bibliography . 21 BS EN ISO 8655-7:2005iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of natio
20、nal standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Internati
21、onal organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rul
22、es given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at
23、least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 8655-7 was prepared by Technical Committee ISO/TC
24、 48, Laboratory glassware and related apparatus, Subcommittee SC 6, Laboratory and volumetric ware. ISO 8655 consists of the following parts, under the general title Piston-operated volumetric apparatus: Part 1: Terminology, general requirements and user recommendations Part 2: Piston pipettes Part
25、3: Piston burettes Part 4: Dilutors Part 5: Dispensers Part 6: Gravimetric methods for the determination of measurement error Part 7: Non-gravimetric methods for the assessment of equipment performance BS EN ISO 8655-7:2005vIntroduction The ISO 8655 series was developed in order to specify the diffe
26、ring types of piston-operated volumetric apparatus and to provide a reference method and alternative test methods for verifying their characteristics covering the volume range typically from: the smallest hand-held pipetting devices, e.g. 1 l, up to the largest laboratory bench-standing volume dispe
27、nsing instruments, e.g. 100 ml. ISO 8655-1 provides general requirements and terminology. The detailed volumetric ranges for each type of apparatus specified in the ISO 8655 series are indicated in the appropriate tables of maximum permissible error, i.e. for piston pipettes (ISO 8655-2), for piston
28、 burettes (ISO 8655-3), for dilutors (ISO 8655-4) and for dispensers (ISO 8655-5). ISO 8655-6 is the reference method for type testing and conformity testing. It is gravimetric and contains precise instructions designed to limit variation in procedure and thereby the potential for sources of error a
29、 necessity for type and conformity testing. The photometric and titrimetric methods described in this part of ISO 8655, are deliberately given as outline methods (see examples in the informative annexes), so that individual laboratories having their own equipment available, and working to different
30、uncertainty requirements, may adapt either these methods, or the gravimetric method, accordingly. If the laboratories operate under ISO 9000 series regimes, or have accreditation to ISO 17025, the individually-adapted methods are usually validated to give results equivalent to those given by the gra
31、vimetric method specified in ISO 8655-6. This part of ISO 8655 is applicable to the following types of testing: of piston-operated volumetric apparatus for purposes other than type testing or the conformity testing which is required prior to declarations or certification of conformity; in user locat
32、ions, where there may be no suitable balance or facilities to perform the reference method given in ISO 8655-6, but which may have at their disposal a suitable photometer or automatic titrator. As users have expressed the wish to have alternative tests available, the following observations are given
33、 to help them select the most appropriate test methods for their purposes. a) Gravimetric method: Uncertainty values can increase at volumes significantly below 1 l, due to increasing balance uncertainty, especially in low humidity areas (where there is increased risk of evaporation) and due to the
34、effects of static electricity. These effects are compensated for through the careful design of the test method specified in ISO 8655-6, which applies to the volume ranges specified in ISO 8655-2 to ISO 8655-5. b) Photometric method: This may be the method of choice for laboratories having a UV/VIS p
35、hotometer of suitable wavelength and bandwidth. Uncertainty with this method tends to become lower as test volumes decrease and can be further reduced if the volumes used in dilution steps for the preparation of comparative standards use larger capacity Class A glassware (e.g. 100 ml of chromophore
36、solution diluted to 1 000 ml can lead to lower uncertainty than 10 ml diluted to 100 ml). c) Titrimetric method: This may be the method of choice of a laboratory already having a titrator with the properties specified in 6.2 and C.4.1. in Annex C. The method is most suited to the testing of piston-o
37、perated volumetric apparatus working in the volume range above 500 l. Again, uncertainty can be reduced if larger capacity Class A volumetric apparatus and larger weights of solid reagents are used to prepare standard solutions. BS EN ISO 8655-7:2005vi If any of these methods is adapted, the expande
38、d uncertainty of measurement needs to be calculated to enable comparison with the reference method. In any case, users will determine that the uncertainty of the chosen method is suitable for their intended purpose. The tests specified in the ISO 8655 series are intended to be carried out by trained
39、 personnel. BS EN ISO 8655-7:20051Piston-operated volumetric apparatus Part 7: Non-gravimetric methods for the assessment of equipment performance WARNING The use of this part of ISO 8655 may involve hazardous materials, operations and equipment. This standard does not purport to address all the saf
40、ety problems associated with its use. It is the responsibility of the user of this part of ISO 8655 to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1 Scope This part of ISO 8655 specifies the photometric and titrimetric det
41、ermination of errors of measurement of piston-operated volumetric apparatus. The tests are applicable to complete systems comprising the basic apparatus and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery process. These non-gravimetric t
42、est methods can be applied as aids to quality assurance by the supplier, as routine quality assurance and routine calibrations by the user, and as routine and post-repair testing. The methods described in this part of ISO 8655 are not applicable as alternatives to the gravimetric reference test meth
43、ods specified in ISO 8655-6, which gives the only method suitable as a basis for suppliers declarations or independent certification of conformity. NOTE 1 Metrological requirements for piston-operated volumetric apparatus, especially maximum permissible errors, are specified in ISO 8655-2 to ISO 865
44、5-5. NOTE 2 For conformity tests or type tests for declaration and certification of conformity, see the gravimetric reference test methods in ISO 8655-6. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the e
45、dition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 648, Laboratory glassware One-mark pipettes ISO 1042, Laboratory glassware One-mark volumetric flasks ISO 3696, Water for analytical laboratory use Specification and te
46、st methods BS EN ISO 8655-7:20052 ISO 8655-1, Piston-operated volumetric apparatus Part 1: Terminology, general requirements and user recommendations ISO 8655-2, Piston-operated volumetric apparatus Part 2: Piston pipettes ISO 8655-3:2002, Piston-operated volumetric apparatus Part 3: Piston burettes
47、 ISO 8655-4, Piston-operated volumetric apparatus Part 4: Dilutors ISO 8655-5, Piston-operated volumetric apparatus Part 5: Dispensers ISO 8655-6:2002, Piston-operated volumetric apparatus Part 6: Gravimetric methods for the determination of measurement error 3 Terms and definitions For the purposes
48、 of this part of ISO 8655, the terms and definitions given in ISO 8655-1 apply. 4 Principle 4.1 Photometric method The photometric method of testing piston-operated volumetric apparatus relies upon the relationship between the concentration of a chromophore in solution and its absorbance of light at
49、 a specified wavelength, as described by the Beer-Lambert law. The method can use one of two procedures, depending on the needs of the calibration. In both methods, the test volume of liquid to be measured is delivered by the piston-operated volumetric apparatus under test into a known volume of liquid, and the degree of dilution is calculated from photometric measurements. The first method is suitable for test volumes 20 % of the total volume. The total volume depends on the size of the photometric meas
