1、BSI Standards PublicationBS EN ISO 9308-1:2014Water quality Enumerationof Escherichia coli and coliformbacteriaPart 1: Membrane filtration method forwaters with low bacterial background floraBS EN ISO 9308-1:2014 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN I
2、SO 9308-1:2014. It supersedes BS EN ISO 9308-1:2000 which is withdrawn.The UK committee voted against this standard on the grounds that the method is insufficiently selective because it enables too many background organisms to grow making reading of the plates problematic. This method is only useful
3、 for water with very low bacterial counts. It is the opinion of the UK committee that more appropriate membrane filtration methods are available for the monitoring of drinking water, including for regulatory purposes. These methods, approved by the Drinking Water Inspectorate, are described in The M
4、icrobiology of Drinking Water (2009) - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7) (https:/www.gov.uk/government/publications/standing-committee-of-analysts-sca-blue-books).Alternatively BS ISO 9308-2:2012 Water quality - E
5、numeration of Escherichia coli and coliform bacteria - Part 2: Most probable number method may also be used.The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/4, Microbiological methods.A list of organizations represented on this su
6、bcommittee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2014. Published by BSI Standards Limited 2014ISBN 978 0 580 71143 5ICS
7、07.100.20; 13.060.70Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 October 2014.Amendments/corrigenda issued since publicationDate Text affectedEUROPEAN STAN
8、DARD NORME EUROPENNE EUROPISCHE NORM EN ISO 9308-1 September 2014 ICS 07.100.20 Supersedes EN ISO 9308-1:2000English Version Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014) Qua
9、lit de leau - Dnombrement des Escherichia coli et des bactries coliformes - Partie 1: Mthode par filtration sur membrane pour les eaux faible teneur en bactries (ISO 9308-1:2014) Wasserbeschaffenheit - Zhlung von Escherichia coli und coliformen Bakterien - Teil 1: Membranfiltrationsverfahren fr Wsse
10、r mit niedriger Begleitflora (ISO 9308-1:2014) This European Standard was approved by CEN on 24 August 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any altera
11、tion. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made
12、by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia
13、, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDAR
14、DIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 9308-1:2014 EBS EN ISO 9308-1:2014EN I
15、SO 9308-1:2014 (E) 3 Foreword This document (EN ISO 9308-1:2014) has been prepared by Technical Committee ISO/TC 147 “Water quality“ in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a na
16、tional standard, either by publication of an identical text or by endorsement, at the latest by March 2015, and conflicting national standards shall be withdrawn at the latest by March 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
17、 rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 9308-1:2000. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this Europea
18、n Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Sloven
19、ia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 9308-1:2014 has been approved by CEN as EN ISO 9308-1:2014 without any modification. BS EN ISO 9308-1:2014EN ISO 9308-1:2014 (E) 3 Foreword This document (EN ISO 9308-1:2014) has been prepared by Techni
20、cal Committee ISO/TC 147 “Water quality“ in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest
21、 by March 2015, and conflicting national standards shall be withdrawn at the latest by March 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such pa
22、tent rights. This document supersedes EN ISO 9308-1:2000. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, F
23、inland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text o
24、f ISO 9308-1:2014 has been approved by CEN as EN ISO 9308-1:2014 without any modification. BS EN ISO 9308-1:2014EN ISO 9308-1:2014 (E) 3 Foreword This document (EN ISO 9308-1:2014) has been prepared by Technical Committee ISO/TC 147 “Water quality“ in collaboration with Technical Committee CEN/TC 23
25、0 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by March 2015, and conflicting national standards shall be withdrawn at the latest by March
26、 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 9308-1:2000. According to the CEN-CENELEC Inter
27、nal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ir
28、eland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norwa , Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 9308-1:2014 has been approved by CEN as EN ISO 9308-1:2014 without any modification. B
29、S EN ISO 9308-1:2014ISO 9308-1:2014(E) ISO 2014 All rights reserved iiiContents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 25 Apparatus and glassware 26 Culture media and reagents 27 Sampling 38 Procedure. 38.1 Preparation of the sample . 3
30、8.2 Filtration . 38.3 Incubation and differentiation 39 Expression of results 410 Test report . 411 Quality assurance 411.1 General . 411.2 Performance testing of Chromogenic Coliform Agar (CCA) . 411.3 Performance testing of oxidase test 5Annex A (informative) Further microbiological information on
31、 coliform bacteria 6Annex B (normative) Composition and preparation of culture media and reagents 7Annex C (informative) Performance characteristics 9Bibliography .10BS EN ISO 9308-1:2014ISO 9308-1:2014(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of n
32、ational standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Inter
33、national organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those inte
34、nded for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www
35、.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document w
36、ill be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expres
37、sions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 4, M
38、icrobiological methods.This third edition cancels and replaces the second edition (ISO 9308-1:2000), which has been technically revised.It also incorporates the Corrigendum ISO 9308-1:2000/Cor.1:2007.ISO 9308 consists of the following parts, under the general title Water quality Enumeration of Esche
39、richia coli and coliform bacteria: Part 1: Membrane filtration method for waters with low bacterial background flora Part 2: Most probable number method Part 3: Miniaturized method (Most Probable Number) for the detection and enumeration of E. coli in surface and waste wateriv ISO 2014 All rights re
40、servedBS EN ISO 9308-1:2014ISO 9308-1:2014(E)IntroductionThe presence and extent of faecal pollution is an important factor in assessing the quality of water and the risk to human health from infection. Examination of water samples for the presence of Escherichia coli (E. coli), which normally inhab
41、its the bowel of man and other warm-blooded animals, provides an indication of such pollution. Examination for coliform bacteria can be more difficult to interpret because some coliform bacteria live in soil and surface fresh water and are not always intestinal. Therefore, the presence of coliform b
42、acteria, although not a proof of faecal contamination, may indicate failure in treatment, storage, or distribution. ISO 2014 All rights reserved vBS EN ISO 9308-1:2014ISO 9308-1:2014(E) BS EN ISO 9308-1:2014Water quality Enumeration of Escherichia coli and coliform bacteria Part 1: Membrane filtrati
43、on method for waters with low bacterial background floraWARNING Persons using this document should be familiar with normal laboratory practice. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish app
44、ropriate safety and health practices and to ensure compliance with any national regulatory conditions.IMPORTANT It is absolutely essential that tests conducted in accordance with this document be carried out by suitably qualified staff.1 ScopeThis part of ISO 9308 specifies a method for the enumerat
45、ion of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growt
46、h can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.This part of ISO 9308 is especially suitable for waters with low bacterial numbers that will cause less than 100
47、 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.Some strains of E. coli which are -D-glucuronidase negative, such as Escherichia coli O157, will not be detected as E. coli. As they are -D-
48、galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For
49、undated references, the latest edition of the referenced document (including any amendments) applies.ISO 3696, Water for analytical laboratory use Specification and test methodsISO 7704, Water quality Evaluation of membrane filters used for microbiological analysesISO 8199, Water quality General guidance on the enumeration of micro-organisms by cultureISO 11133, Microbiology of food, animal feed and water Preparation, production, storage and performance testing of culture mediaISO 19458, Water quality Sampling for microbiological
