1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN ISO 11269-1:2012Soil quality Determination of the effects of pollutants on soil floraPart 1: Method for the measurement of inhibition of root growthIncorporating corrigendu
2、m March 2013BS EN ISO 11269-1:2012 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN ISO 11269-1:2012. It supersedes BS ISO 11269-1:2012, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee EH/4, Soil quality.A list of o
3、rganizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2013.Published by BSI Standards Limited
4、 2013ISBN 978 0 580 78526 9 ICS 13.080.30 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 28 February 2013.Amendments/corrigenda issued since publicationDate Tex
5、t affected28 March 2013 This corrigendum renumbers BS ISO 11269-1:2012 as BS EN ISO 11269-1:2012EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11269-1 December 2012 ICS 13.080.30 English Version Soil quality - Determination of the effects of pollutants on soil flora - Part 1: Method for th
6、e measurement of inhibition of root growth (ISO 11269-1:2012) Qualit du sol - Dtermination des effets des polluants sur la flore du sol - Partie 1: Mthode de mesurage de linhibition de la croissance des racines (ISO 11269-1:2012)Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die
7、 Bodenflora - Teil 1: Verfahren zur Messung der Wurzelwachstumshemmmung (ISO 11269-1:2012) This European Standard was approved by CEN on 11 November 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the st
8、atus of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French,
9、German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cro
10、atia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
11、United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 1126
12、9-1:2012: EForeword The text of ISO 11269-1:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 11269-1:2012 by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat
13、 of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2013, and conflicting national standards shall be withdrawn at the latest by June 2013. Attention is drawn to the pos
14、sibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries
15、are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
16、Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 11269-1:2012 has been approved by CEN as a EN ISO 11269-1:2012 without any modification. BS EN ISO 11269-1:2012 EN ISO 11269-1:2012 (E)2 ISO 2012Soil quality Determina
17、tion of the effects of pollutants on soil flora Part 1: Method for the measurement of inhibition of root growthQualit du sol Dtermination des effets des polluants sur la flore du sol Partie 1: Mthode de mesurage de linhibition de la croissance des racinesINTERNATIONAL STANDARDISO11269-1Second editio
18、n2012-03-01Reference numberISO 11269-1:2012(E)ii ISO 2013COPYRIGHT PROTECTED DOCUMENT ISO 2012All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, withou
19、t permission in writing from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.orgPublished in SwitzerlandBS EN ISO 11269-1:2012 I
20、SO 11269-1:2012 (E) ISO 2013 iiiContents PageForeword ivIntroduction v1 Scope 12 Normative references . 13 Terms and definitions . 14 Principle . 25 Test plants . 36 Materials . 36.1 Test vessels 36.2 Soil . 37 Equipment . 58 Reference substance 59 Procedure 59.1 Experimental design . 59.2 Preparati
21、on of pots 69.3 Pregermination of the seeds 69.4 Growing conditions 69.5 Test duration . 69.6 Measurements 710 Expression of results and data 711 Validity criteria . 712 Test report . 7Annex A (informative) Recommended method for measuring the water-holding capacity of the soil. . 9Annex B (informat
22、ive) Results of tests performed on reference substances . 10Annex C (informative) Example of results obtained with boric acid using sand as the substrate 11Annex D (informative) Recommended methods for the incorporation of chemicals into soils . 12Annex E (informative) Recommended methods for the in
23、corporation of compost, sludge or waste into soils 13Annex F (informative) Example of seedlings of winter barley collected at the end of the test after removal from artificial soil 15Bibliography .16BS EN ISO 11269-1:2012ISO 11269-1:2012 (E)ForewordISO (the International Organization for Standardiza
24、tion) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be
25、represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.International Standards
26、 are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Internati
27、onal Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.ISO 11269-1 was p
28、repared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods.This second edition cancels and replaces the first edition (ISO 11269-1:1993), which has been technically revised.ISO 11269 consists of the following parts, under the general title Soil quality Determinati
29、on of the effects of pollutants on soil flora: Part 1: Method for the measurement of inhibition of root growth Part 2: Effects of contaminated soil on the emergence and early growth of higher plantsiv ISO 2013BS EN ISO 11269-1:2012 ISO 11269-1:2012 (E)IntroductionChemical analysis of soil samples or
30、 waste materials to be disposed on soil, together with ecotoxicological testing, provides substantial evidence of the suitability of the soil for arable production, or gives information on the potential environmental risk resulting from the disposal of wastes such as sewage sludge on farmland. There
31、 is also a need to assess the quality of the soil after reclamation of industrial sites and colliery tips or when capping landfill sites. As the ability of the soil to grow crops is the main criterion, a rapid-growth test has been developed, based on seedling growth in controlled environmental condi
32、tions.Two major prerequisites of a phytotoxicity test are that it provides consistently reliable results and that it can be used at any time of the year. It is therefore essential that seeds be grown in a controlled environment to ensure optimal growing conditions which can be maintained for any num
33、ber of tests, producing reproducible results over a long period of time.The test method described in this part of ISO 11269 can be used to compare soils, to monitor changes in their activity or to determine the effect of added chemicals or materials (compost, sludge, waste). ISO 2013 vBS EN ISO 1126
34、9-1:2012 ISO 11269-1:2012 (E)Soil quality Determination of the effects of pollutants on soil flora Part 1: Method for the measurement of inhibition of root growth1 ScopeThis part of ISO 11269 describes a method for the determination of the effects of contaminated soils or contaminated samples on the
35、 root elongation of terrestrial plants.This method is applicable to soils, soil materials, compost, sludge, waste or chemical testing. It is applicable to the comparison of soils of known and unknown quality and to the measurement of effects of materials (compost, sludge, waste) or chemicals deliber
36、ately added to the soil.The method is not intended to be used as a measure of the ability of the soil to support sustained plant growth.2 Normative referencesThe following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applie
37、s. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and divers
38、ity in the laboratoryISO 10390, Soil quality Determination of pHISO 10694, Soil quality Determination of organic and total carbon after dry combustion (elementary analysis)ISO 10930, Soil quality Measurement of the stability of soil aggregates subjected to the action of waterISO 11260, Soil Quality
39、Determination of effective cation exchange capacity and base saturation level using barium chloride solutionISO 11268-1, Soil quality Effects of pollutants on earthworms Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andreiISO 11268-2, Soil quality Effects of pollutants on earthwo
40、rms Part 2: Determination of effects on reproduction to Eisenia fetida/Eisenia andreiISO 11277, Soil quality Determination of particle size distribution in mineral soil material Method by sieving and sedimentationISO 11465, Soil quality Determination of dry matter and water content on a mass basis G
41、ravimetric methodISO/TS 20281, Water quality Guidance on statistical interpretation of ecotoxicity data3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1 contaminant substance or agent present in the soil as a result of human activ-ityINTERNATIONA
42、L STANDARD ISO 2013 1BS EN ISO 11269-1:2012 ISO 11269-1:2012 (E)3.2 test mixture mixture of test soil or test material (compost, sludge, waste or chemical) with control soil3.3 radicle portion of the plant embryo which develops into the primary root3.4 hypocotyl portion of the axis of an embryo or s
43、eedling situated between the cotyledons (seed leaves) and the radicle3.5 reference soil uncontaminated site-specific soil (e.g. collected in the vicinity of a contaminated site) with similar properties (nutrient concentrations, pH, organic carbon content and texture) as the test soil3.6 standard soi
44、l field-collected soil or artificial soil whose main properties (e.g. pH, texture, organic matter content) are within a known rangeEXAMPLE Euro-soils1, artificial soil2, LUFA soil.1)NOTE The properties of standard soils can differ from the test soil.3.7 control soil reference or standard soil used a
45、s a control and as a medium for preparing dilution series with test soil or test material (e.g. compost, sludge, waste, chemical)NOTE Both the effective concentration (ECx) and the no-observed-effect concentration (NOEC) are expressed in milligrams of test substance per kilogram (dry mass) of the te
46、st substrate. Soil mixtures are given in percent based on soil dry mass.3.8 effective concentration ECx effective concentration (dilution) of the test soil or test material (e.g. compost, sludge, waste, chemical) at which root elongation is reduced by x % compared to the control4 PrincipleThis metho
47、d compares the root elongation of terrestrial plants in a test soil and/or a series of dilutions with a control soil. This method may also be used for the testing of compost, sludge, waste or chemicals by applying various concentrations of the material under investigation to a control soil.Pregermin
48、ated seeds are exposed to the test material under controlled conditions. After the growth period, the lengths of the roots of the test plants are compared with those of the control plants. Statistically significant differences in the root lengths of seedlings grown in any test medium compared to the
49、 controls are indicative of an effect.NOTE Shoot height is also a useful parameter, and this can be measured in conjunction with root length to provide additional or corroborative data.1) Euro-soils, artificial soil and LUFA soil are examples of suitable products available commercially. This information isgiven for the convenience of users of this document and does not constitute an endorsement by ISO of these products.2 ISO 2013BS EN ISO 11269-1:2012 ISO 11269-1:2012 (E)5 Test plantsWinter barley
