1、 FORD LABORATORY TEST METHOD AM 150-03 Date Action Revisions 2001 01 30 Revised Editorial no technical change A. Cockman 2000 04 14 Editorial no technical change A. Cockman 1991 09 23 Printed copies are uncontrolled Page 1 of 4 Copyright 2001, Ford Global Technologies, Inc. MINERAL OIL CONTAMINATION
2、 OF BRAKE FLUID BY THIN LAYER CHROMATOGRAPHY Application This procedure describes a method for detecting and estimating mineral oil contaminants above 0.1 percent in brake fluids by thin layer chromatography. Apparatus Required Thin Layer Plates Size 200 x 200 mm, Silica Gel coated, layer thickness
3、0.25 mm. Source: Andermann CAUTION: Highly dangerous fire hazard. FORD LABORATORY TEST METHOD AM 150-03 Page 2 of 4 Copyright 2001, Ford Global Technologies, Inc. Solutions Required Spray Reagent Solution 0.1 % w/v sodium fluorescein in ethanol. CAUTION: Low flash point. Standard Brake Fluid Solutio
4、ns Contaminated with 0.1 % v/v mineral oil. ASTM No. 3 to be used as contaminant. Note: 0.05 % and 0.025 % v/v mineral oil contaminated standards are also used for new oil evaluations. Conditioning and Test Conditions All test values indicated herein are based on material conditioned in a controlled
5、 atmosphere of 23 +/- 2 C and 50 +/- 5 % relative humidity for not less than 24 h prior to testing and tested under the same conditions unless otherwise specified. Procedure 1. Using a pencil, mark a base line on the thin layer plate, 25 mm from the edge, the plate being later immersed with this edg
6、e in the developing solvent. Using the spotting guide for accurate location, apply on the base line, with the syringe, 2 individual spots, i.e. 10 microliters of the brake fluid under test and 10 microliters of the standard 0.1 % v/v mineral oil contaminated brake fluid. 2. Prepare the developing ta
7、nk by lining with filter paper saturated with the developer solution, ensuring that there is approximately 15 mm depth of solution in the tank. Note: Tank must be cleaned and solution changed once per two days minimum. The test fluid spots must not be immersed in the developing solution. 3. Immerse
8、the plate with the edge, mentioned above, in the developing tank. Allow to stand for 1 h at 23 +/- 2 C, ensuring that tank is sealed by means of the lid. 4. Remove the plate from the tank, and allow developer solution to volatilize. 5. When dry, place under a fume hood and spray the plate with the f
9、luorescein spray reagent in such a way that the spraying unit is being traversed over the plate to produce a 50 % overlapping from one spray operation to the other. The operation must be repeated until uniform coat is achieved. FORD LABORATORY TEST METHOD AM 150-03 Page 3 of 4 Copyright 2001, Ford G
10、lobal Technologies, Inc. 6. Expose under the U.V. lamp until the spot produced from the sample of the standard mineral oil contaminated brake fluid becomes visible. Note: Wavelength 254 gives optimum resolution. Evaluation Mineral oil contamination is detected by the presence of a dark spot or tail
11、on the fluorescent background of the thin layer plate. The size and intensity of the mineral oil spot is compared with that of the brake fluid spot under test. Chemicals, materials, parts, and equipment referenced in this document must be used and handled properly. Each party is responsible for dete
12、rmining proper use and handling in its facilities. FORD LABORATORY TEST METHOD AM 150-03 Page 4 of 4 Copyright 2001, Ford Global Technologies, Inc. MINERAL OIL CONTAMINATION OF BRAKE FLUID BY THIN LAYER CHROMATOGRAPHY Note: Intensity of contaminant spot is normally visible only after development with Sodium Fluorescein.
