1、FORD LABORATORY TEST METHOD BN 012-03 2001 0822 RESISTANCE OF VINYLS TO MILDEW (“PINK STAINING) Revised Editorial - no technical change A. Cockman Application This procedure is used to determine the resistance of vinyls to mildew growth which may cause a “pink“ staining condition in the field. Appar
2、atus Required Dishes 4 inch Petri dishes and covers. Incubator Capable of maintaining a temperature of 86 +/- 3 OF Sterilizing Equipment Autoclave capable of maintaining 15 Ibs. of steam pressure for 20 minutes. Refrigerator maintained at 40 OF. Inoculating Needle Solutions Required Stock Culture St
3、reptomyces Rubrireticuli - NRRL B-1484“ (Store in refrigerator at 40 OF) *Source: United States Dept. of Agriculture ARS Culture Collection Investigations Fermentation Laboratory 181 5 North University Street Peoria, Illinois 61604 I Date I Action I Revisions I Copynght02001, Ford Global Technologie
4、s, Inc FORD LABORATORY TEST METHOD BN 012-03 H i c key-Tres na rs Ag ar Yeast Extract Beef Extract N-Z Amine“ Dextrin Cobaltous Chloride Agar-Agar Distilled Water Final pH 1 .O gm 1 .O gm 2.0 gm 10.0 gm 20.0 mg 20.0 gm 1000 mL 7.3 *Source: Sheffield Chemical Co. Norwich. New York Note: The stock cul
5、tures of Streptomyces Rubrireticuli are maintained on Hickey Tremers Agar whereas Bennetts Agar is used as a plating medium. Bennetts Agar Yeast Extract Beef Extract N-Z Amine Glucose Agar-Agar Distilled Water Final pH 1 .O gm 1 .O gm 2.0 gm 10.0 gm 15.0 gm 1000 mL 7.3 Wetting Agent Triton XI00 Cond
6、itioning and Test Conditions All test values indicated herein are based on material conditioned in a controlled atmosphere of 23 +/- 2 “C and 50 +/- 5 % relative humidity for not less than 24 h prior to testing and tested under the same conditions unless otherwise specified. Page 2 of 3 Copynght0200
7、1, Ford Global Technologies, Inc FORD LABORATORY TEST METHOD BN 012-03 Procedure Sterilize Bennetts Agar medium in autoclave under 15 Ibs. pressure for a minimum of 20 minutes. Pour sufficient medium to cover bottom of a sterile Petri dish and cool to 23 +/- 2 OC. The inoculum shall be prepared by a
8、dding a few drops of non-toxic wetting agent (Triton X-100) to a 7 - 14 day old culture of the test organism growing in a Petri dish, or growing on an Agar test tube slant (Hickey-Tresnar). One loopful of the mycelium is streaked in a cross hatched pattern on the culture medium (Bennetts Agar). Cut
9、two samples 3/4 inch in diameter. One sample is placed with the vinyl side down on the Agar. The textile side of the second sample should first be treated in the following manner: Place two drops of sterilized water on the fabric and work the drops with a sterilized glass rod until the fabric is com
10、pletely wet. Now place the fabric side on the Agar. Care should be taken to establish good contact between the surface of the samples and the plating medium. (The use of sterilized stainless steel weights less than 3/4 inch in diameter may prevent curling of the samples.) A control sample should be
11、included in each Petri dish. Incubate the Petri dishes at 86 +/- 3 OF for a period of two weeks. Observe the degree of staining on the samples and compare with the control. Note: Standard microbiological practice should be observed when conducting this test. Chemicals, materials, parts, and equipment referenced in this document must be used and handled properly. Each party is responsible for determining proper use and handling in its facilities. Page 3 of 3 Copynght02001, Ford Global Technologies, Inc
