1、 Pseudomonas spp.ISO 13720:2010Meat and meat products Enumeration of presumptive Pseudomonas spp.(IDT) 137202011 27 2002 . 184- , 1.02004 . 1 - . . - ( . . ) , 42 226 3 13 2011 . 943-4 O 13720:2010 . Pseudomonas spp. (ISO 13720:2010 Meat and meatproducts Enumeration of presumptive Pseudomonas spp.).
2、 - , - 5 - , - . () . - , , 2014 , - - II 1372020111 12 13 14 25 , 26 .37 .48 49 410 511 .5 () .6 .7 137202011III Pseudomonas spp.Meat and meat products. Enumeration of presumptive Pseudomonas spp. 201301011 , , - Pseudomonas spp.2 : 6887-11999 . , . 1. (ISO 6887-1:1999Microbiology of food and anima
3、l feeding stuffs Preparation of test samples, initial suspension and decimaldilutions for microbiological examination Part 1: General rules for the preparation of the initial suspensionand decimal dilutions) 6887-22003 . , . 2. - (ISO 6887-2:2003 Microbiology of food andanimal feeding stuffs Prepara
4、tion of test samples, initial suspension and decimal dilutions for microbiologicalexamination Part 2: Specific rules for the preparation of meat and meat products) 7218 . - (ISO 7218 Microbiology of food and animal feedingstuffs General requirements and guidance for microbiological examinations) 111
5、33-1 . - . 1. - (ISO/TS 11133-1 Microbiology of food and animal feedingstuffs Guidelines on preparation and production of culture media Part 1: General guidelines on qualityassurance for the preparation of culture media in the laboratory) 11133-2 . - . 2. - (ISO/TS 11133-2 Microbiology of food and a
6、nimal feeding stuffs Guidelines onpreparation and production of culture media Part 2: Practical guidelines on performance testing of culturemedia)3 : Pseudomonas spp. (presumptive Pseudomonas spp.): , - - - (CFC ) 25 .1 1372020114 , - CFC Pseudomonas spp. 25 (44 c177 4) . Pseudomonas spp. (). Pseudo
7、monas spp. 1 (3) - F .5 , 5.1 O 7218; 11133-1 11133-2.5.2 6887-1 6887-2.5.3 - - (CFC ) 15.3.1 5.3.1.1 :- 16,0 ;- .10,0 ;- (K2SO4).10,0 ;- (MgCl2).1,4;- * .12,0 18,0 ;- 1000 3.5.3.1.2 . (. 6.4) , - 7,2 c177 0,2 25 . - (. 6.6) (. 6.1) 121 15 .5.3.2 (5 c177 3) 7 .5.3.2.1 5.3.2.1.1 : .0,1; .1003.5.3.2.1
8、.2 .5.3.2.2 5.3.2.2.1 : .0,1; .1003.5.3.2.2.2 -.5.3.2.3 5.3.2.3.1 :* 0,1; .1003.* .* - () .2 1372020115.3.2.3.2 .5.3.3 5.3.3.1 , 3, /3 (. 5.3.1) .100.; (. 5.3.2.1) 550; (. 5.3.2.2) .110; (. 5.3.2.3).110.5.3.3.2 , (. 6.3) (47 c177 2) , .5.3.4 CFC (. 6.8) 15 3 -. 11133-1. (5 c177 3) .5.3.5 11133-1. FC
9、 11133-2.5.3.5.1 : 25 (44 c177 4) .: Pseudomonas fluorescens WDCM 00115* Pseudomonas flagi WDCM 00116*. : - (TSA). : .: PR 0,5.5.3.5.2 : 25 (44 c177 4) .: Escherichia coli WDCM 00013*. : .: .5.4 5.4.1 :N, N, c162N , c162N -p- 1,0 ; 100 3.5.4.2 . ( ) . - .6 - 7218, :6.1 .6.2 , (25 c177 1) .6.3 , (47
10、c177 2) .6.4 - c177 0,05 .6.5 , - - .* , http:/www.wfcc.nig.ac.jp/WDCM Reference Strain Catalogue, . .3 1372020116.6 , .6.7 , , 1 3, 0,1 3 2 3, .6.8 90 100 .6.9 , , 3,5 , 200 , 30 , .7 . , - , - . , , - (. 7218).8 6887-1 6887-2 / , , - .9 9.1 , 6887-2.9.2 9.2.1 - 7218. .9.2.2 0,1 3 FC (. 5.3.4). FC 0,1 3 . , - .9.2.3 (. 6.9) , .9.2.4 (. 6.2) 25 - (44 c177 4) .9.3 , 150 . .9.4 9.4.1 (. 5.4.2). - - 6.5 (- ) . 510 , . 30 , -.4 137202011 , . Pseudomonas aeruginosa WDCM 00025( ), Escherichia coli WDCM 00013 ( ).9.4.2 Pseudomonas spp.10 7218.11 :a) , ;b) ;c) ;d) , - , .5 137202011 () .1
