1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2005 12 30 KS H 3012 KS H 3012: 2005 H 3012: 2005 ( ) ( ) ( ) ( ) F&B ( ) ( ) : : 1989 11 7 : 2005 12 30 2005 1157 : : ( ) ( 02 509 7262 5) . 7 5 , . ICS 67.120.20 KS H 3012: 2005Liquid egg 1. , . 2. . . KS A 7002 KS H 1101 KS H 1203 KS H 1204 3. . 3.1 , ,
2、5 . 3.2 20 . 3.3 . 3.4 . 3.5 , , 5 . 3.6 20 . 3.7 . 3.8 . 3.9 , , 5 . 3.10 20 . 4. 4.1 4.1.1 (, ) 4.1.2 4.1.3 4.1.4 4.2 4.2.1 (, ) H 3012: 2005 2 4.2.2 4.2.3 4.2.4 4.3 4.3.1 (, ) 4.3.2 5. 5.1 1 . 1 , . % 12.0 13.5 10.5 % 10.0 27.0 0.1 % 24.0 43.0 11.0 pH(1) 7.1 7.8 6.1 6.5 8.5 9.5 (2) 10 000 /g (2)
3、10 /g (2) (1) . (2) . 5.2 1 . 6. 6.1 KS A 7002 3.( ) . 6.2 KS H 1204( ) 3.( ) , 1 5.71, 2 6.25 . 6.3 KS H 1203 ( ) 4.( ) . 6.4 ( 50 mm .) 15 g , 98 100 , 3 4 g . ( 1 0.000 2 g .) . =%)( 100 6.5 pH pH . H 3012: 2005 3 6.6 6.6.1 40 , 10 mL 90 mL 30 cm 25 . 6.6.2 1 mL 10 1 mL 2 43 45 (platecount agar)
4、15 mL . 3 5 mL . 20 . (351) 24 48 . 1 mL , , . 15 % . 9 10 cm, 1.5 cm . . 5 24 . ( 1/2 .) 1 30 300 . 300 300 9 cm 1 cm2 65 . 30 . 6.6.3 a) (sodium chloride) 8.5 g 1 000 mL 15(121 ) 15 . b) (plate count agar, ) (tryptone) 5.0 g (yeast extract) 2.5 g (dextrose) 1.0 g (agar) 15.0 1 000 mL , pH 7.0 15(1
5、21 ) 15 . 6.7 6.6 10 , 100 1 mL . 1 mL 3 EC (44.50.2) (242) . . EC 1 EMB (351) (242) , . (351) (483) (351) (242) . , . 6.7.1 H 3012: 2005 4 a) 8.5 g 1 000 mL 15 (121 ) 15 . b) EC (EC broth) (peptone) 20.0 g (lactose) 5.0 (bile salt mixture) 1.5 g (dipotassium phosphate) 4.0 g (monopotassium phosphat
6、e) 1.5 g (sodium chloride) 5.0 g 1 000 mL , pH 6.9 7.1 15 (121 ) 15 . c) EMB (EMB agar) (peptone) 10.0 g (lactose) 5.0 g (sucrose) 5.0 g (dipotassium phosphate) 2.0 g Y(eosin Y) 0.4 g (methylene blue) 0.065 g (agar) 13.5 1 000 mL , pH 7.2 15(121 ) 15 . d) (lactose broth) (peptone) 5.0 g (beef extrac
7、t) 3.0 g (lactose) 5.0 g 1 000 mL , pH 6.9 15 (121 ) 15 . e) (nutrient agar) 1 000 mL 15 g . pH 7.0 7.4 15 (121 ) 15 . 6.8 1 2 mL Selenite F broth 10 mL 35 37 48 . Desoxycholate Citrate Agar MacConkey Agar 35 37 24 . 35 37 18 24 TSI (371) 18 24 . . . . , . . a) 0 0 . b) 0 0 , A, B, C, D, E H 3012: 2
8、005 5 . c) H H , a, b, c, d, e, h, G, K, L, r, y . 6.8.1 a) 34 g 500 mL , 1 N 175 mL pH 7.2 1 000 mL . 121 15 . 1 mL 800 mL . b) 8.5 g 1 000 mL 121 15 . c) 40 mL 70 1.0 2.0 g . 6 mL . . 7. 6. , 5.1 8. . 8. 8.1 . 8.2 . 9. 9.1 KS H 1101( ) 9.( ) . 9.2 KS H 1101( ) 6.( ) . 9.3 KS H 1101 ( ) 11.( ) . 20
9、06 1 24 135 513 701 7 (02)6009 4567 (02)6009 4887 8 http:/ 607 822 1 11( 10 ) (051)557 1239 Fax. (051)557 0430 702 012 2 1741( 3 ) (053)384 1562 4 Fax. (053)384 1565 790 380 627( ) (054)278 9611 Fax. (054)278 9662 406 130 994( 16 ) (032)260 0260 7 Fax. (032)260 0268 443 766 906 5( 9 ) (031)259 7000
10、9 Fax. (031)259 7010 200 041 1 9( 304 ) (033)252 9423, 254 9423 Fax. (033)256 9423 361 802 1508 1( 6 ) (043)236 2451 3 Fax. (043)236 2454 305 343 23 14( 5 ) (042)864 2301 3 Fax. (042)864 2304 330 816 43 5( 1204 ) (041)589 0500 3 Fax. (041)589 0504 561 841 1 337 2( 7 ) (063)214 2234 7 Fax. (063)214 2
11、238 506 301 621 15( 4 ) (062)953 1435 7 Fax. (062)953 1438 641 210 298 7 ( 6 ) (055)212 1212 Fax. (055)212 1213 683 804 758 2( 2 ) (052)289 6601 3 Fax. (052)289 6604 425 020 541 3( 4 ) (031)487 6191 4 Fax. (031)487 6195 730 350 92 30( 8 ) (054)473 6954 Fax. (054)473 6955 480 848 2 487 6( 3 ) (031)829 8182 4 Fax. (031)829 8185 KOREAN STANDARDS ASSOCIATION KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKS KS H 3012: 2005 Liquid egg ICS 67.120.20