1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 11 20 http:/www.kats.go.kr KS H ISO 14378 ( )KS H ISO 14378： 2006 (2011 ) H ISO 14378： 2006 : ( ) ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 2001 12 31 ： 2006 11 20 ： 2011 12 29 2011-0652 ： ： ( 02-509-7273) (http:/www.kats.go.kr). 10 5 , . ICS 67.100
2、.10 KS ( ) H ISO 14378： 2006(2011 ) Milk and dried milk Determination of iodide content Method using high-performance liquid chromatography 2000 1 ISO 14378 Milk and dried milk Determination of iodide content Method using high-performance liquid chromatography . ISO 14378 ISO/TC 34 (SC 5), (IDF) (AO
3、AC International) , . ISO , . , . . 1. ( 0.031 g/g) ( 0.310.0 g/g) (HPLC) . 1. , . 2. 510 % . . 2. . . KS M ISO 3696 3. . 3.1 . g/g . 4. 25 000 D (cut-off membrane) . 050 mV HPLC . . H ISO 14378： 2006 2 5. HPLC . 5.1 KS M ISO 3696 2 . 5.2 . 5.2.1 100 mg/L (KI) 130.8 mg 1 000 mL (6.2) . . 5.2.2 20 g/
4、L, 50 g/L, 150 g/L 250 g/L . (5.2.1) 20 L, 50 L, 150 L 250 L 4 100 mL (6.2) . . 5.3 (CH3CN) HPLC . 5.4 3 CH3(CH2)15N(CH3)3Cl 25 %( ) , . 5.5 HPLC (68： 32 ) 2 3 pH 6.8 . 2 (Na2HPO4) 1.42 g 1 000 mL (6.2) 600 mL . 3 (5.4) 1.3 mL (5.3) 320 mL . (H3PO4) pH 6.8 . 1.2 m 0.5 m . (sonification) 2 . . 1 . 5.
5、6 n- 6. . 6.1 0.01 g . 6.2 100 mL 1 000 mL . 6.3 25 L, 50 L, 150 L 250 L . 6.4 0.1 mL 2 mL . 6.5 500 mL . 6.6 pH . 6.7 1.2 m 0.5 m, Nylon-6-6(1) HPLC . (1) 6-6, Amicon CS1A, Amicon centreflo and Millipore Ultrafre-PF . 6.8 50 mL 1 000 g . 6.9 50 mL , 27 mm, . 6.10 (6.9) (6.11) Amicon CS1A(1), . 6.11
6、 25 00030 000 D Amicon Centreflo(1) CF 25, . . (2： 8 ) 1 , H ISO 14378： 2006 3 . (6.10) 50 mL 9001 000 g 510 . . . , (1： 5 ) . . Milipore Ultrafre PF(1)(UFP1, 10 000 D ) , . 6.12 HPLC . 6.12.1 2 mL/ . 6.12.2 50200 L . 6.12.3 PARTISPHERE C 18(2), 5 m, 4.7 mm, 110 mm, . (2) PARTISPHERE C 18 Whatman .
7、6.12.4 Guard ( ) SPHERI 5 C 18 cartridge(3), 3.2 mm, 15 mm, . (3) SPHERI 5 C 18 cartridge Brownlee . 6.12.5 0 50 mV . 6.12.6 . “ (negative peak)” Spectra Physics(4) . (4) Spectra Physics . 7. , KS H ISO 707 . . 8. 8.1 . 8.2 (202) . 40 (202) . 8.3 . 9. 9.1 9.1.1 (455) g 0.1 g 100 mL (6.2) . . 9.1.2 (
8、4.20.2) g 0.01 g 100 mL (6.2) . 7080 mL 510 . n- (5.6) . 9.2 (clean-up) (9.1.1 9.1.2) 5 mm 9001 000 g 1520 . ( 2 ) HPLC . 6.11 . 9.3 HPLC H ISO 14378： 2006 4 9.3.1 HPLC HPLC (5.3) (5.1) (1： 1 ) HPLC (5.5) 30 mL . 2 mL/ 1 . (6.12.5)( 0 50 mV, 1020 nA) HPLC (5.5) . 250 g/L (5.2.2) 50 L . , 3 % . 48 ,
9、(5.5 ). 0 50 mV ( 1 ). 80 % 250 g/L (5.2.2) . HPLC (5.5) . 0.2 mL/min . (5.3) (5.1) (1： 1 ) HPLC HPLC (5.5) . 9.3.2 4 (5.2.2) . 5 . . (9.2 ) . 5 . . 68 150 g/L (5.2.2) . 5 % . HPLC . . 9.4 4 ( ) . (0.0) . 0.99 . 10. 10.1 . wI= 1.0tmw wI： (g/g) wt： (g/L) m： (g) 10.2 10.2.1 0.1 g/g , 0.11 g/g . 10.2.2
10、 1 g/g , 110 g/g . H ISO 14378： 2006 5 11. 11.1 ISO 5725(5) . (5) ISO 5725： 1986( ) . 11.2 2 25 % 5 % . 11.3 2 30 % 5 % . 12. . ( ) a) (102 g/L) b) (2.3 g/L) 1 2 4.7 mm, 110 mm PARTISPHERE C 18 5 m LC 1 H ISO 14378： 2006 6 1 KS H ISO 707 2 ISO 5725： 1986 Precision of test methods Determination of re
11、peatability and reproducibility for a standard test method by inter-laboratory tests 3 KS A ISO 5725 1： 2002 ( ) 1： 4 KS A ISO 5725 2： 2002 ( ) 2： 5 SERTL D. and MALONE W. J. AOAC. Int., 76, 1993, pp. 711 719 6 AOAC International, Official methods of analysis, 16th edition, 1995, Method No. 992.22 ( ) 153787 1 92 3(13) (02)26240114 (02)2624 01489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS H ISO 14378： 2006 Milk and dried milkDetermination of iodide contentMethod using high performance liquid chromatography ICS 67.100.10
