1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS H ISO 20370 , KS H ISO 20370:2012 2012 9 19 http:/www.kats.go.krKS H ISO 20370:2011 : ( ) ( ) () () ( ) : (ISO/TC126) () ( ) () KT&G () KT&G () () ( ) KT&G : (http:/www.standard.go.kr) ： ：2012 9 19 2012-0452 ： ：(ISO/TC126) ( 02-509-7266) (http:/www.kats.g
2、o.kr). 10 5 , . KS H ISO 20370:2012 i ii . iii 1 1 2 1 3 .1 4 2 5 2 5.1 2 5.2 2 5.3 (sodium acetate trihydrate) .3 6 .3 7 4 7.1 4 7.2 .4 8 .5 9 6 10 .6 7 KS H ISO 20370:2012 ii ISO(the International Organization for Standardization) (ISO ) . ISO . . ISO . ISO (IEC) . ISO/IEC , 2 . . . 75 % . . ISO .
3、 ISO/TC 126, . KS H ISO 20370:2012 iii 2009 1 ISO 20370, Material used for producing wrappings for cigarette filters, cigarettes and other tobacco products Determination of acetate content . KS H ISO 20370:2012 , Material used for producing wrappings for cigarette filters, cigarettes and other tobac
4、co products Determination of acetate content , . . . 1 , (acetate) . 2 . . ( ) . KS M ISO 187, , KS M ISO 287, KS M ISO 3696, 3 . 3.1 , , . KS H ISO 20370:2012 2 4 , ( ) 3 (adenosine 5-triphosphate, ATP) A(coenzyme A, CoA) A (acetyl coenzyme A synthase, ACS) A(acetyl coenzyme A), 1 (adenosine 5-mono
5、phosphate, AMP) (pyrophosphate) . ACS acetate ATP CoA acetyl-CoA AMP pyrophosphate(1) A(acetyl coenzyme A) (citrate synthase, CS) (oxaloacetate) . CS acetyl-CoA oxaloacetateH2O citrateCoA (2) (2) L- (L-malate dehydrogenase, L-MDH) - (nicotinamide-adenine dinucleotide, NAD) . L-MDH malate NADoxaloace
6、tate H NADH(3) NADH 340 nm . , NADH . 5 5.1 (analytical grade) . KS M ISO 3696 3 . 5.2 5.2.1 (kit) Roche-Biopharm 10.148.261.035 1). , . , . 5.2.2 1 1) Roche-Biopharm 10.148.261.035 . , ISO . KS H ISO 20370:2012 3 (ready-to-use) 1 (triethanolamine) pH 8.4 , . L- (L-malic acid), 4.2 mg/mL (magnesium
7、chloride), 2.1 mg/mL 1 4 . 5.2.3 2 2 2 . 2 . 3 (adenosine 5-triphosphate, ATP), 25.0 mg/mL A(coenzyme A, CoA), 2.6 mg/mL (nicotinamide adenine dinucleotide, NAD), 12.3 mg/mL 2 4 4 . 5.2.4 3 3 . L- (L-malate dehydrogenase, L-MDH), 2 750 IU2)/mL (citrate synthase), 675 IU/mL 3 4 . (1005) % . 5.2.5 4 4
8、 4 . 4 A (acetyl coenzyme A synthase, ACS) 20 IU/mL . 4 4 5 . 5.3 (sodium acetate trihydrate) 6 . 6.1 , 250 mL . 6.2 , 80 mm . 2) IU ( ) 1 1 mol ( ) . KS H ISO 20370:2012 4 6.3 , 125 mmWhatman No. 40 .3) 6.4 , 1 mL, 2 mL, 5 mL, 10 mL , (enzyme assay pipettes) 2. 6.5 , 10 L 20 L . 6.6 (double-beam sp
9、ectrophotometer), 340 nm . 6.7 (cuvets), 10 mm , 5 mL . 6.8 (ultrasonic bath) (magnetic stirrer) 6.9 , 0.001 g . 7 7.1 KS M ISO 187 1.0 g 0.001 g 250 mL (6.1) 100 mL (6.8) 30 . (6.3) . 7.2 20 25 . (pipetting) ( 1 ) ( ) (7.1 ) . ( ) 340 nm (6.6) . , V 3.23 mL . , 300 mg/L, 100 mg/L 50 mg/L (5.3) . 3)
10、 Whatman No.40 . , ISO . KS H ISO 20370:2012 5 1 mL mL 5.2.2 1 1.00 1.00 5.2.3 2 0.20 0.20 2.00 1.90 0.10 , A0 . 5.2.4 3 0.01 0.01 , 3 , 1A . 5.2.5 4 0.02 0.02 , ( 10 15 ) , 2A . 15 , 2 2 . , . 8 NADH , , A (4. ). . ()()()()()()=blank022blank01blank02sample022sample01sample02AAAAAAAAAAAAA (4) 0.1 .
11、1 g 15 g , 0.01 g/L 0.15 g/L . 0.2 0.4 . L g A . AVFMVA =0001P(5) , V ： (mL)( 3.23 mL) M ： F ： ： 340 nm NADH ： 6.30 L mmol 1 cm 1 ： (cm) VP： (mL) A ： 7.2 , L g , A (M 60.05 g/mol) . A 2.566A(6) A KS H ISO 20370:2012 6 . %100PAA= (7) P , L g . 1 g 100 mL , P 10 g/L , A . A A10 % (8) ( ) . 9 2 sr sR .
12、 0.4 % 1.1 % 3 2005 9 . 8 . 2 mNsrsR , n A1 8 0.52 0.025 0.031 A2 8 0.40 0.035 0.043 A3 8 1.11 0.037 0.057 10 . a) ( , , ) b) c) d) e) f) g) KS M ISO 287 h) i) j) , . KS H ISO 20370:2012 7 1 CORESTA Recommended Method No 33, Determination of Acetate in Cigarette Paper, January 1993 2 DIN 12699, Labo
13、ratory glasswareGraduated pipettes for enzymatic analyses, short delivery time, waiting time 15s, class AS , 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS H ISO 20370:2012 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Material used for producing wrappings for cigarette filters, cigarettes and other tobacco products Determination of acetate content ICS 65.160
