1、KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 11 17 http:/www.kats.go.krKS I ISO 10260 a KS I ISO 10260: 2006 (2011 ) I ISO 10260: 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) : :1996 1 5 :2006 11 17 :2011 12 26 2011-0623 : : ( 02- 509-7270) (http:/www.kats.go.kr). 10 5 , . ICS 13.060.50 KS
2、 I ISO a 10260: 2006(2011 ) Water quality Measurement of biochemical parameters Spectrometric determination of the chlorophyll-a concentration 1992 1 ISO 10260 Water quality Measurement of biochemical parametersSpectrometric determination of the chlorophyll-a concentration . 0. - a . . - a . . . 1.
3、1.1 - a . , . ( A ). 1.2 - b - c . - a . 1.3 . . . 1.4 75 5 . ( .) 4 3 . 25 30 . 1.5 . 665 nm 750 nm . 1.6 ( ) - a . 665 nm - b - c . 2. . . . IEC ISO . I ISO 10260: 2006 2 KS M ISO 5667 1 1: KS M ISO 5667 2 2: 3. . - a . , 665 nm - a . 4. . 4.1 c(HCI) 3 mo1/L 4.2 C2H5OH, 90 % (V/V) (90 %) . 5. 5.1
4、1 nm, 2 nm , 0.001 , 1 5 cm 750 nm 5.2 5.3 1 m 99 % . 25 50 mm 5.4 5.3 . 25 mm. 6 000 g 5.5 (751) . 5.6 , 30 50 mL , 6 000 g polytetrafluoroethylene(PTFE) (vials) 6. KS M ISO 5667 1 KS M ISO 5667 2 . 8 . 7.1 7.3 . 25 30 (5.6) . . 7. 7.1 . Vs( 0.1 2 L ) (5.3) . , . . . 7.2 A (4.2) 75 . ( 30 40 mL) ,
5、. (4.2) . 3 I ISO 10260: 2006 3 . . , . (5.4) (50 mL 100 mL ) . (4.2) , . . Ve . 7.4 . 7.3 B (4.2) VE( 20 25 mL) (5.6) . . . (5.5) . 5 . 15 . . 1. (7.4). 3 . (5.4) (5.6) . ( 2. ). . . 2. . Ve . (7.1 ) , 5 % . 7.4 7.4.1 (7.4.2 ), (cuvette) . (4.2) 665 nm(A665) 750 nm(A750) . 665 nm 0.01 0.8 . , , , .
6、 0.5 L, 50 mm , 20 mL 5 cm . 7.4.2 ( 5 10 mL) 10 mL (4.1) 0.01 mL . 5 30 , 665 nm 750 nm . 8. 8.1 - a c . dVVRRKAA=se3cac101)( (1) A A665 A750: (7.4.1 ) Aa A665 A750: I ISO 10260: 2006 4 Ve: (mL) Vs: (L) Kc 82 L/g cm:- a R 1.7: - a A/Aa d: (cm) 103: Ve 8.2 p . cse3cap10 =dVVKRA (2) 8.3 (90 %) - a 82
7、 - a (R) 1.7 , - a c dVVAA=seac6.29)( (3) . (2) (4) . cseap8.20 =dVVA (4) 1. - a . - a 5 11 % , 6 46 % . 2. - a A/Aa 1.7 . -a 1 . (4.2) - a (82) 665 nm . (84) - b - c . - a 665 nm 2 3 % . 8.4 ( ) 2 . - a 5.5 g/ L 0.1 g /L 9. 1983 1 . I ISO 10260: 2006 5 1 l n na% g/L r g/L VCr% Rg/L VCR% A 18 71 0 1
8、26.5 5.46 4.3 6.36 5.0 B 18 70 2.8 20.3 3.67 18.1 2.90 11.3 C 17 67 5.6 24.6 2.21 9.0 2.8 11.4 l: r: n: VCr: na: R: : VCR: 10. . a) b) c) 8. d) , e) x xI ISO 10260: 2006 6 A() A.1 KS M ISO 5667 1 KS M ISO 5667 2 . ( ) ( , substratum) . . . . . . A.2 1966 UNESCO SCOR . . , . . , , . ( ) . . - a . - a
9、 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 10260: 2006 Water qualityMeasurement of biochemical parameters-Spectrometric determination of the chlorophyll -a concentration ICS 13.060.50