1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 11 17 http:/www.kats.go.krKS I ISO 15089 KS I ISO 15089： 2006 (2011 ) I ISO 15089： 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) ： ：2001 12 18 ：2006 11 17 ：2011 12 26 2011-0623 ： ： ( 02- 509-7270) (http:/www.kats.go.kr). 10 5 , . ICS 13.060.50 K
2、S I ISO 15089： 2006(2011 )Water quality Guidelines for selective immunoassays for the determination of plant treatment and pesticide agents 2000 1 ISO 15089 Water quality Guidelines for selective immunoassays for the determination of plant treatment and pesticide agents . 1. , , ( ) . 0.05 g/L . 0.0
3、5 g/L . 2. . . . IEC ISO . ISO 5667 1： 1980 Water quality Sampling Part 1： Guidance on the design of sampling programmes ISO 5667 2： 1991 Water quality Sampling Part 2： Guidance on sampling techniques ISO 5667 3： 1994 Water quality Sampling Part 3： Guidance on the Preservation and handling of sample
4、s ISO/TR 13530： 1997 Water quality Guide to analytical quality control for water analysis 3. 3.1 (affinity) Ab H AbH (K) . Ab , H ( ), K K cAbH/(cAbcH) . 3.2 (analyte) 3.3 (antibody) , . 1. (mAb) . . 2. (pAb) . . 3. (rAb) . 3.4 (antibody conjugate) . 3.5 (antigen) . I ISO 15089： 2006 2 3.6 (antiseru
5、m) . / . 3.7 (coating conjugate) . . 3.8 (competitive immunoassay) . . . 3.9 (cross-reactivity) . 1. . (100 % ) . IC50 . . . . . . 2. . 3.10 (enzyme immunoassy) EIA ( ) . . . 3.11 (enzyme substrate) . 3.12 (excess standard) . 3.13 (fluorescence immunoassay) . 3.14 (hapten) . . 3.15 (heterogeneous im
6、munoassay) . 3.16 (immunoassay) , . 3.17 (immunogen) . 3.18 (inhibition concentration) IC 50 % IC50 (100 %) . 3.19 (luminescence immunoassay) LIA . 3.20 (solid phase immunoassy) I ISO 15089： 2006 3 . ELISA(enzyme-linked immunosorbent assay) . 3.21 (tracer) , . 3.22 (zero standard) . 4. (interference
7、) . . , , , pH . . 5. (principle) . . . ( 1 4 ). EIA . ( ) . . . . . . 6. 6.1 . . . 10 % . 10 % . . . 6.2 (buffered washing solution) 80 mmol/L(NaH2PO4, Na2HPO4) 85 g/L . 6.3 (acid) c(H2SO4) 1 mol/L pH . 6.4 (base) c(NaOH) 1 mol/L pH . 7. 7.1 (solid phase) , , , , . I ISO 15089： 2006 4 7.2 (multipip
8、ettes) , 10 500 L , 10 L, 100 L, 200 L , , 300 L . 7.3 (magnetic rack ) , . 8. 8.1 . , . 8.2 (coating of solid phases) . . a) , 50 mmol/L (pH 9.6) , . ELISA . b) . ELISA . . , . . 8.3 (enzyme tracer) . . 8.4 , . 8.5 , , , . 20 37 (0.5 ) . pH ( , ) . . 9. . . 9.1 ( ) 9.1.1 (binding reaction) . 30 60
9、. 9.1.2 (washing step) . 9.1.3 (enzyme reaction) . . . . 9.1.4 . I ISO 15089： 2006 5 IA(immunoassay) . 9.1.5 A (pipetting) . 9.1.5.1 200 L . . , 1： 1 000 10 mL 10 L . 50 L 30 . 20 1 . 9.1.5.2 300 L 3 . . 9.1.5.3 , 10 15 200 L . 10 100 L . 10 . . . 9.1.5.4 (microwell plate) (photome ter) . (well) 1.0
10、 1.5 . . 3 10 % . . 9.2 . (6.1) . . 9.3 . . 3 % . , ： 0 g/L, 0.03 g/L, 0.10 g/L, 0.30 g/L, 1.0 g/L, 10 g/L, 1 000 g/L 5 . . 10. (validity criteria) . . . (CV) . CV 10 % . . . I ISO 15089： 2006 6 11. (calculation) 11.1 (replicate) 5 10 % . CV . 3 . 11.2 B/B0 . , . , , (1) B/Bo . . B/Bo 100)()(eooAAAA
11、(1) B： Bo： A： Ao： Ae： 11.3 (2) ( 6 ). . 1 . y d+50IC1dab (2) y： d： a： ： (g/L) IC50： IC50 (g/L) b： IC50 12. . B. EIA . 13. . a) b) c) d) 9.3 e) 11.3 f) 10. g) , h) I ISO 15089： 2006 7 1 ( 5 ) EIA 1.75 0.75 0.50 0.25 0.75 75.75 50.75 25.75 0.75 1.75 0.75 1.75 2.75 3.75 4.75 0.01 0.1 1 10 g/L% B/BoLogi
12、t I ISO 15089： 2006 8 A() (pipetting) ( ) , 30 60 ( ) 10 30 A.1 A.1 1 , 200 L 2 50 L 3 30 1 4 300 L(3) 5 200 L 10 30 6 (H2SO4) 100 L 7 I ISO 15089： 2006 9 B() B.1 2 3 . B.1 EIA l n o % xnom g/L x g/L % R g/L CVR % r g/L CVr % 1 15 56 11.1 0.003 0.001 6 52.8 0.006 18.9 2 15 57 9.5 0.130 0.125 95.8 0.
13、015 3 12.3 0.008 0 6.4 3 14 54 8.5 0.115 0.116 100.5 0.023 4 20.2 0.010 9 9.4 l ： n ： o ： xnom ： x ： R ： CVR ： r ： CVr ： 1： 2： 0.130 g/L 3： 0.115 g/L I ISO 15089： 2006 10 1 GEE S.J., Hammock B.D. and Skerritt J.H. Diagnostics for plant agrochemicals a meeting of chemistry and immunoassay. In: Biotec
14、hnology in Agriculture Series: New Diagnostics in Crop Sciences(Skerritt, J.H. and Appels, R., sds.) CAB International, United Kingdom, 1995, pp. 243 276 2 Sherry J. Environmental chemistry： The immunoassay option. Crit. Rev. Anal. Chem., 23, 1992, pp. 217 300 3 HOCK B. Enzyme immunoassays for pesti
15、cide analysis. Acta hydrochim. Hydrobiol., 21, 1993, pp. 71 93 4 MEULENBERG E.P., MULDER W.H. and STOKS P.G. Immunoassays for pesticides. Environ. Sci. Technol., 29, 1995, pp. 553 561 5 GIERSCH T. A new monoclonal antibody for the sensitive detection of atrazine with immunoassay in microtiterplate a
16、nd dipstick format. J. Agric. Food Chem., 41, 1993, pp.1006 1011 6 DUDLEY R.A., EDWARDS P., EKINS R.P., FINNEY D.J., MCKENZIE I.G.M., Raab G.M., RODBRD D. and RODGERS R.P.C. Guidelines for immunoassay data processing. Clin. Chem., 31, 1995, pp. 1264 1271. 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 15089： 2006 Water qualityGuidelines for selective immunoassays for the determination of plant treatment and pesticide agents ICS 13.060.50
