1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 9439 KS I ISO 9439:2008 2008 11 24 http:/www.kats.go.krKS I ISO 9439:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 2003 12 3 ： 2008 11 24 2008-0794 ： ： ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 9439:2008 Water quality Evaluation o
2、f ultimate aerobic biodegradability of organic compounds in aqueous medium Carbon dioxide evolution test 1999 2 ISO 9439, Water quality Evaluation of ultimate aerobic biodegradeability of organic compounds in aqueous medium Carbon dioxide evolution test , . ISO 9439 ISO/TC 147, , SC5, . A D . . . .
3、1 (CO2) , . . a) , DOC ( D ). b) , (： KS I ISO 10634 ). c) . KS I ISO 9408 KS I ISO 14593 d) . 8.3 , (： KS I ISO 8192 ). 2 KS I ISO 9439:2008 2 2.1 (ultimate aerobic biodegradation) , ( ) 2.2 (primary biodegradation) ( ) 2.3 (activated sludge) 2.4 (concentration of suspended solids) 105 2.5 (dissolv
4、ed organic carbon) DOC 15 40 000 m s 2 0.2 m 0.45 m 2.6 (total inorganic carbon) TIC 2.7 (dissolved inorganic carbon) DIC 15 40 000 m s 2 0.2 m 0.45 m 2.8 (theoretical amount of formed carbon dioxide) ThCO2 (mg)/ (mg g) 2.9 (lag phase) KS I ISO 9439:2008 3 10 % 2.10 (maximum level of biodegradation)
5、 . . 2.11 (biodegradation phase) 90 % 2.12 (plateau phase) ( ) 2.13 (pre-exposure) , 2.14 (preconditioning) , 3 . , , 10 mg/L 40 mg/L , . 28 ( A) . ( B), (ThCO2) (%) . DOC . , D . . 4 KS I ISO 9439:2008 4 2 20 25 (diffused light) . 5 . 5.1 1 mg/L DOC . 5.2 5.2.1 a) a) (5.1) 1 000 mL . (KH2PO4) 8.5 g
6、 (K2HPO4) 21.75 g 2 (Na2HPO4 2H2O) 33.4 g (NH4Cl) 0.5 g (5.1) 1 000 mL . pH , pH 7.4 . , . b) b) 7 (MgSO4 7H2O) 22.5 g (5.1) 1 000 mL . c) c) 2 (CaCl2 2H2O) 36.4 g (5.1) 1 000 mL . d) d) (III) 6 (FeCl3 6H2O) 0.25 g (5.1) 1 000 mL . (HCl) 1 . 5.2.2 1 000 mL 800 mL (5.1), 10 mL a) 1 mL b) d) . (5.1) 1
7、 000 mL . 6 . 6.1 ( , ) . ( )KS I ISO 9439:2008 5 . 6.2 3 L 50 mL/min 100 mL/min , ( A ). 6.3 , DIC ( B ) 6.4 (DOC) ( ) 6.5 (0.2 m 0.45 m) . 6.6 pH 7 7.1 7.1.1 (5.1) (5.2) , 10 mg/L 40 mg/L . ( , ) . . . KS I ISO 10634 . 7.1.2 . 20 mg/L (7.1.1) , (5.2) . 7.1.3 (5.2) 20 mg/L (7.1.1) (7.1.2) . 7.2 7.2
8、.1 KS I ISO 9439:2008 6 (7.2.2) 7.2.3 7.2.4 . (7.1.2 9. ) . (9. ) . ： (5.2.2) 7 . ( 2 ). 1 . , (： x %), . ：Zahn Wellens (KS I ISO 9888) SCAS (ISO 9887) (： ) . 2 . . (9. ) . 1 mL 103CFU 106CFU . 30 mg/L . DOC 10 % . 1 mL 10 mL 1 000 mL . 7.2.2 . ( KS I ISO 11923 ) . . . 30 mg/L . 7.2.3 , . . . 1 . 7.
9、2.4 . . . 7.3 (6.1) . (7.1.1) ( FT) ( FB) KS I ISO 9439:2008 7 (7.1.2) ( FC) (7.1.3) ( FI) , (7.1.1) ( FS). (HgCl2) 10 g/L 1 mL/L . 2 . 3 L 1 (5.2) (7.2) . . . ( A ). (4.) 24 , . . . (7.1.1) 1 (7.1.2) 50 mL/min 100 mL/min 3 L . 1 (5.2) (7.1.1) (7.1.2) (7.2) FT FT FB FB FC FI ( ) FS ( ) ( B ) . ( ) .
10、 28 . 1 2 . pH , 1 mL 10 mL . 24 . 1 , (DIC) , . , . (FS ) . KS I ISO 9439:2008 8 FS . 2 (DOC) D . 8 8.1 (ThCO2, mg) (1) . 1244ThCOLC2 V (1) C： (mg/L) , (7.1.1) . VL： L . 44 12 ： . (7.1.3) ThCO2 . 8.2 , % (2) FT Dm . 2BTThCOttmmDm (2) tmT ： t FT (mg) tmB ： t FB (mg) FC . FI , FS . DOC D . 8.3 (tmT t
11、mB) (Dm) % . , % . . FT (20 % ) , . C FC , FI , FS KS I ISO 9439:2008 9 . ( ) , % “ ” , . . FI % 25 % , . . FS (10 % ) . . FS (10 % ) . 9 9.1 . a) FC( ) (%) 14 60 % b) 3 L FB 40 mg/L 70 mg/L c) (DIC) 5 % a) b) . c) . 9.2 FI , FI , % 40 % . . 9.3 pH pH 6.0 8.5 (%) 60 % , D . 10 . a) b) c) , ( ) d) Th
12、CO2 , DOC e) KS I ISO 9439:2008 10 f) , , , g) h) i) DOC (%) j) , FS (%) k) , FI (%) l) m) KS I ISO 9439:2008 11 A ( ) ( ) A.1 . 50 mL/min 100 mL/min . . . , NaOH (c 10 mol/L) 500 mL . Ba(OH)2 (c 0.012 5 mol/L) 100 mL . . B . 1 2 3 (NaOH) 4 Ba(OH)2 5 6 7 NaOH Ba(OH)2 A.1 1 2 3 4567 KS I ISO 9439:2008 12 B ( ) B.1 (DIC) NaOH , DOC DIC . NaOH(c 0.05 mol/L) . DIC B . DIC ( 10 mL). NaOH . DIC . (B.1) . 1067.3)(BTTtm (B.1) tmT： t FT (mg) T： t FT NaOH DIC (mg/L) B： t FB DIC (mg/L) 3.67 ： (44/12) 10 ： NaOH 100 mL L , . B.2 (B.2). Ba(OH)2 (B.3). CO2 Ba(OH)2 BaCO3 H2O (B.2) Ba(OH)2 2HCI BaCl2 2H2O
