1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 9509 KS I ISO 9509:2008 2008 11 24 http:/www.kats.go.krKS I ISO 9509:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 1998 3 26 ： 2008 11 24 2008-0794 ： ： ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 9509:2008 Water quality Method for a
2、ssessing the inhibition of nitrification of activated sludge micro organisms by chemicals and wastewaters 2006 2 ISO 9509, Water quality Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms , . . . . . , . . 1 . 3 24 . , . N (N allylthiourea); A . , g 2 mg 6
3、.5 mg 2 mg N/g h 6.5 mg N/g h , , (9. ). , . . . 3 24 (KS M ISO 5667 16) . KS I ISO 9509:2008 2 2 . . ( ) . KS I ISO 5667 16, 16： KS I ISO 6777, KS I ISO 7150 1, 1： KS I ISO 11733, 3 , . 3.1 (nitrification) . ISO 6107 1： 2004, 49. 3.2 (test material) , , 3.3 (activated sludge) ( ) ISO 6107 1： 2004,
4、2. 3.4 (concentration of suspended solids of an activated sludge) 105 KS I ISO 9888, 3.4 3.5 (toxic range) 0 % 100 % ISO 8192:1) 1) (ISO 8192:1986 )KS I ISO 9509:2008 3 3.6 EC50 50 % 4 ( ) . 5 5.1 . . ( , Cu2 ) . 5.2 ( C ) . , . ( 6 ). . ( 1 ), 24 (KS I ISO 5667 16 ). , ( C ). . . ( 5 10 000 min 1)
5、. . , ( 3 g/L) . 5.3 (NaHCO3) 5.04 g (NH4)2SO4 2.65 g 1 L . (5.1) 1： 10(1 9) , 56 mg/L , pH 7.6 . pH 25 mg/L . 5.4 5.4.1 A KS I ISO 9509:2008 4 (NaHCO3) 10.08 g (5.1) 1 L . 5.4.2 B (NH4)2SO4 5.3 g (5.1) 1 L . 5.5 N allythiourea(ATU) 1.16 g (5.1) 1 L . 2 6 ( ) 2-chloro-6(trichloromethl)pyridine , . 5
6、.6 (5.1) (1 g/L 10 g/L) . , pH 7.60.1 . 5.7 4 (KS I ISO 5667 16 ). , pH 7.60.1 . (ammonium N) . , . 100 mg/L . , N 20 mg/L . , (heterotrophic bacteria) . , 56 mg/L (batch) . B(5.4.2), A(5.4.1), , A, B . 6 6.1 6.1.1 200 mL 500 mL 6.1.2 100 mL 6.2 KS I ISO 9509:2008 5 6.3 6.3.1 100 mL (6.1.2) 6.3.2 (6
7、.1.1) . 6.4 6.5 . 6.6 / . 7 7.1 , A . 3 2 mg 6.5 mg( 2 mg N/g h 6.5 mg N/g h) . (5.1) (9. ). , . , ( 6.1.1 6.1.2) (VF) 1/10 / (5.3) . 1 500 mg/L (VF/2) (5.2) . , , 5 mL (5.6) (5.1) (VF) ( B ). . , , , , , ATU (5.5) VF/100, 11.6 mg/L . , . ( ) , VF/10 (5.3) VF/20 A(5.4.1) , (VF/2) . , VF , 56 mg/L (
8、) B(5.4.2) (5.1) . 7.2 KS I ISO 9509:2008 6 (222) 4 ( 2 mg N/g h ) , (6.1.2) 4 mg/L (6.1.1) . 4 mg/L . ( )(： KS I ISO 6777, ISO 7890 1) / ( , ： KS I ISO 7150 1) , (6.4, 6.5) . (20 mL 25 mL) . . . 8 (IN%) . IN (c t)/(c b)100 (1) c ： , (mg/L) t： , (mg/L) b： , (mg/L) t . (INH3) . . INH3 (l e)/(o e)100
9、(2) l： , (ammonium N) (mg/L) e： , (ammonium N) (mg/L) o： (ammonium N) (mg/L) EC50 . EC50 . 9 . , ( A ), . , . , . , KS I ISO 9509:2008 7 . 2 mg N/g h 6.5 mg N/g h . 2 mg N/g h “ ”, 6 10 d ( C KS I ISO 11733 ). 6.5 mg N/g h , , pH (5.4) . . EC50 . 10 (1985) , 3 6 11 1 . 1 EC50 EC50 mg/L mg/L % mg/L 3
10、,5 Dichlorophenol 5.6 3.0 54 0.7 9.6 10 4 Nitrophenol 43.3 26.7 62 8.4 92 11 N Allylthiourea 0.38 0.23 61 0.1 0.7 6 1 , (ISO 9509:1989) (5.3) (5.1) . 2 3.5 Dichlorophenol(5.6 mg/L) EC50 (0.525 mg/L) 10 ( 3 5 ). Nallylthiourea 2, , .11 . a) (KS I ISO 9509:2008) b) c) d) , e) EC50, , f) g) h) i) KS I
11、ISO 9509:2008 8 A ( ) A.1 2 ( 3 g/L) (5.2) (VF/2) . 10 mL (5.5) VF/100 . VFmL . 2 mg/L 4 . 4 , , / ( ) . , RN mg N/g h . RN ( t b)/(MLSS4) (A.1) t： 4 (mg/L) b： 4 (mg/L) MLSS： (mg/L) . A.2 4 , . RNS(mg NH4 N/g h) . RNS b(NH4 N) t(NH4 N)/MLSS4 (A.2) b(NH4 N) ： 4 (mg/L) t(NH4 N) ： 4 (mg/L) KS I ISO 950
12、9:2008 9 B ( ) B.1 . B.1 No. 1 2 3 4 5 6 7 (5.3)(mL) 25 25 25 25 25 25 25 (5.2)(mL) 125 125 125 125 125 125 125 ATU(5.5)(mL) 0 0 0 0 0 0 2.5 (5.1)(mL) 100 99.75 99.2 97.5 92 75 97.5 a(5.6)(mL) 0 0.25 0.8 2.5 8.0 25 0 (mg/L) 0 1 3.2 10 32 100 0 (mL) 250 250 250 250 250 250 250 3.0 g/L a ： 1 g/L KS I
13、ISO 9509:2008 10 C ( ) C.1 , . C.2 (Husmann unit)(KS I ISO 11733 ) . . . . / . . . . , 50 mg/L 150 mg/L (DOC) . NaHCO3 pH , , K2HPO4 NH4Cl . C.3 ： 20 L ： 10 L ： 3 L ： 99 % ： 15 25 ： C.4 NH4Cl 178.3 g K2HPO429.7 g NaHCO3485 g 5 L . C.5 ( ) ： 100 g KS I ISO 9509:2008 11 1 L . C.6 , 30 L 500 mL (C.4) ,
14、 60 mL . DOC 50 mg/L , . 24 L . (HRT) 0.83 , 1.4 . C.7 (SRT) 2.5 g/L . . 6 g SRT 18 12.5 L . C.8 DOC DOC (%) . DOC 80 % . (9. ). (%) (9. A ) . 4 . (1 2 ) . KS I ISO 9509:2008 12 1 GENDIG, C., DOMOGALA, G., AGNOLI, F., PAGGA, U. and STROTMANN, U.J. Evaluation and further development of the activated
15、sludge respiration test. Chemosphere, 52(1), 2003, pp. 143 149 2 PAINTER, H.A. A review of literature on inorganic nitrogen metabolism in micro-organisms. Wat. Res., 4, 1970, pp. 393 450 3 COLLEY, A., WADHIA, K. and THOMPSON, K.C. Evaluation of two enhanced chemiluminescence test kits for water qual
16、ity and toxicity testing. In： Toxic impacts of wastes on the aquatic environment (TAPP, J.F, HUNT, S.M. and WHARFE, J.R. eds.). Royal Society of Chemistry, Cambridge, UK, 1996, pp 282 3 4 STROTMANN, U.J. and EGLSER, H. The toxicity of substitued phenols in the nitrification inhibition test and lumin
17、escent bacteria test. Ecotox. Env. Saf., 30, 1995, pp. 269 273 5 THOMLINSON, T.G., BOON, A.G., TROTMAN, C.N.A. Inhibition of nitrification inthe activated sludge process of sewage disposal. J. Appl. Bacteriol., 29： 1966, pp. 266 291 6 WINTHER NIELSEN, M. and LACOUR JANSEN, J. The role of the sludge in nitrofication inhibition tests Wat.Sci.Tech.,
