1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS L ISO 27447 KS L ISO 27447:2011 2011 10 28 http:/www.kats.go.krKS L ISO 27447:2011 : ( ) ( ) KCC () ( ) : (http:/www.standard.go.kr) ： ：2011 10 28 2011-0458 ： ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS L ISO 27447:2011 i . ii 1 .1 2 .1 3 .1 4 2
2、5 3 6 3 6.1 .3 6.2 .4 7 6 7.1 6 7.2 6 7.3 .6 7.4 7 7.5 - (black light) 7 7.6 .7 7.7 7 8 8 8.1 8 8.2 8 9 9 9.1 .10 9.2 .11 9.3 .12 9.4 12 10 13 10.1 13 10.2 14 11 .15 A( ) .17 B( ) 19 C( ) 21 22 KS L ISO 27447:2011 23 KS L ISO 27447:2011 ii 2009 1 ISO 27447, Fine ceramics(advanced ceramics, advanced
3、technical ceramics) Test method for antibacterial activity of semiconducting photocatalytic materials , . KS L ISO 27447:2011 Fine ceramics (advanced ceramics, advanced technical ceramics) Test method for antibacterial activity of semiconducting photocatalytic materials 1 (UV) . ( , , ) . , . . ( ,
4、 , , ) . 2 . . ( ) . KS A ISO 31 0, 0 ： KS Q 5002, 1 ： 3 . 3.1 . , , , , . 3.2 , , KS L ISO 27447:2011 2 3.1 . 3.3 3.4 , . 3.5 , . 3.6 3.7 3.8 3.9 3.10 300 nm 400 nm UV-A KS M ISO 4892 3 351 nm (black light blue： BLB) 4 A , BD , BL L , CD , KS L ISO 27447:2011 3 CL L , FBD , FBL L , L Lmax Lmean3 L
5、min M MBA , 3 MBD , 3 MBL L , 3 MD , 3 ML L , 3 N P R RL (L) , R SL (L) , S V (lecithin) (polysorbate) 80 Z 2 5 , , , , . , . , , . . . , . . 6 6.1 6.1.1 a) (Staphylococcus aureus) KS L ISO 27447:2011 4 b) (Escherichia coli) 6.1.2 a) (Staphylococcus aureus) b) (Klebsiella pneumoniae) 6.1.3 1 , . . (
6、 ) . (37 1) (16 24) , 5 10 . 1 2 . 2 10 . 1 . 1 , 2 10 . 2 , . 1 (Staphylococcus aureus) ATCC 6538P DSM 346 NBRC 12732 American Type Culture Collection German Collection of Microorganisms and Cell Cultures (DSMZ) NITE Biological Resource Center (Escherichia coli) ATCC 8739 DSM 1576 NBRC 3972 America
7、n Type Culture Collection German Collection of Microorganisms and Cell Cultures (DSMZ) NITE Biological Resource Center (Klebsiella pneumoniae) ATCC 4352 DSM 789 NBRC 13277 American Type Culture Collection German Collection of Microorganisms and Cell Cultures (DSMZ) NITE Biological Resource Center 6.
8、2 6.2.1 1/500 (nutrient broth) 1 000 mL 3.0 g 10.0 g 5.0 g KS L ISO 27447:2011 5 . , 25 pH (7.1 0.1) . 500 , pH (7.0 0.2) . (121 2) 15 . , , 5 10 . 1/500 1 . 6.2.2 1 000 mL 3.0 g 10.0 g 5.0 g . , 25 pH (7.1 0.1) . (6.2.1 ). , , 5 10 . 1 . 6.2.3 1 000 mL 3.0 g 5.0 g 15.0 g , . . 0.1 mol/L 25 pH (6.8
9、0.2) . (6.2.1 ). , , 5 10 . 1 . 45 48 . 6.2.4 (lecithin) (polysorbate) 80 - (SCDLP) 1 000 mL (casein peptone) 17.0 g, (soybean peptone) 3.0 g, 5.0 g phosphoric acid monopotassium dehydrogenate 2.5 g, (glucose) 2.5 g (lecithin) 1.0 g , . , 7.0 g . 25 pH (7.0 0.2) . (6.2.1 ). , SCDLP , 5 10 . SCDLP 1
10、 6.2.5 1 000 mL 8.5 g . (6.2.1 ). , , 5 10 . 1 . 6.2.6 1 000 mL 8.5 g . , 2.0 g . 20 mL (6.2.1 ). , , 5 10 . 1 . 6.2.7 KS L ISO 27447:2011 6 polyoxyethylene sorbitan monooleate(polysorbate 80) 7 . . 1 2 3 4 5 6 7 1 7.1 , 340 nm 380 nm 85 % . (40 2) mm . B . 7.2 340 nm380 nm 85 % , 1.1 mm . (40 2) m
11、m . B . 7.3 KS L ISO 27447:2011 7 340 nm380 nm 85 % , 1.1 mm . . 7.4 10 cm15 cm , U V . 7.5 - (black light) 351 nm BLB(black light blue) . 7.6 . . 7.7 ( 2, 3 ) . ： mm 1 2 ( 5 mm 15 mm) 2 0.01 mW/cm2 KS L ISO 27447:2011 8 ： mm 1 2 ( 5 mm 15 mm) 3 0.001 mW/cm2 8 8.1 (50 2) mm (50 2) mm . 10 mm . . 9 6
12、 . . . (50 2) mm (50 2) mm(10 mm ) 400 mm2 1 600 mm2 . 24 1.0 mW/cm2 . , ( 70 % ). 8.2 (50 2) mm (50 2) mm . 9 6 . . . (wire-mesh basket) . (autoclave) . , (clean bench) , 60 . KS L ISO 27447:2011 9 9 4 5 . 4 6.1(6.1.1) 2 9.1.1 8.1 9.3.1 (9.3.2 ) 9.1.2 (7. ) 9.1.3 9.3.4 9.1.4 9.3.3 9.1.4 9.4 10.1 11
13、 3. 3. + 3. 3. + 3. KS L ISO 27447:2011 10 5 9.1 9.1.1 (platinum loop) (nutrient agar slant) (37 1) 16 24 . (nutrient agar slant) (37 1) 16 20 . (platinum loop) 1/500 NB , . 6.7 105cells/mL 2.6 106cells/mL 1/500 NB . . , 0 4 . 9.1.2 , . . 0.15 mL . , . . 1 4 mL 6 mL . 2 . . , 9.3.4 9.2.4 6.1(6.1.2)
14、 2 9.2.1 8.2 9.3.1 (9.3.2 ) 9.2.2 (7. ) 9.2.3 9.3.3 9.2.4 9.4 10.2 11. 3. 3. + 3. 3. + 3. KS L ISO 27447:2011 11 1.0 105cells 4.0 105cells . , . 9.1.3 ( ) 3 , (Stomacher bag) . . SCDLP 10 mL , . . , (Stomacher bag) . 9.1.4 9.3.3 9.3.4 9.1.3 . 9.2 9.2.1 . a) (nutrient agar medium) . (37 1) 24 48 5 10
15、 1 ( A). b) 100 mL 20 mL (nutrient culture) . A , (37 1) 1824 (3 cm /110 ) ( B). c) 100 mL 20 mL . 1 108cells/mL2 108 cells/mL B 0.4 mL . 107cells/mL (37 1) (3 1) (3 cm 110 r/min) ( C). d) (optical density absorbance method) (optical microscope observation method) C . 20 , C (1 0.3) 105cells/mL . .
16、 0 4 . 9.2.2 , , . . 0.2 mL . , , . . 1 4 mL6 mL . 2 . . , 1.4 104cells2.6 104cells . . 3 , 0.05 % . KS L ISO 27447:2011 12 . 9.2.3 3 ( ) , , . 20 mL , , . . . 9.2.4 9.3.3 9.3.4 9.2.3 . 9.3 9.3.1 . . 9.3.2 . 9.3.2 . . 2 0.25 mW/cm2 , ( , BLB) 0.10 mW/cm2 ( , 1.5 m ), 0.01 mW/cm2 ( , 3 m ) 0.001 mW/cm2 ( ), ( ) 0.25 mW/cm2 . 0.001 mW/cm2 .
