1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS M ISO 14852 KS M ISO 14852 :2009 2009 11 26 http:/www.kats.go.krKS M ISO 14852:2009 : ( ) ( ) ( )SK ( ) ( ) : (http:/www.standard.go.kr) ： ： 2004 6 30 ： 2009 11 26 2009-0732 ： ： ( 02-509-7300) (http:/www.kats.go.kr). 10 5 , . KS M ISO 14852:2009 Determin
2、ation of the ultimate aerobic biodegradability of plastic materials in an aqueous mediumMethod by analysis of evolved carbon dioxide 1999 1 ISO 14852, Determination of the ultimate aerobic biodegradability of plastic materials in an aqueous mediumMethod by analysis of evolved carbon dioxide . , , .
3、. 1 . , , . , . , . ( C ) . . , , . (KS I ISO 8192 2) . KS M ISO 14852:2009 2 . 2 . . ( ) . KS I ISO 8245, (DOC) (TOC) KS I ISO 9439, ( ) KS I ISO 10634, KS I ISO 15462, 3 . 3.1 (ultimate aerobic biodegradation) () 3.2 (activated sludge) 3.3 (concentration of suspended solids in an activated sludge)
4、 105 3.4 DIC (dissolved inorganic carbon) , 15 40 000 ms 2 0.2 0.45 m 3.5 (theoretical amount of evolved carbon dioxide, ThCO 2 ) , 1 mg 1 g (mg) . 3.6 TOC (total organic carbon) 3.7 DOC (dissolved organic carbon) KS M ISO 14852:2009 3 , 15 40 000 ms 2 0.2 0.45 m 3.8 (lag phase) , 10 % . 3.9 (maximu
5、m level of biodegradation) . . 3.10 (biodegradation phase) 90 % 3.11 (plateau phase) ( ) 3.12 (pre-exposure) , 3.13 (pre-conditioning) , 4 . , 100 2 000 mg/L . , . 6 . , A B . (ThCO 2 ) , . . ( C ). KS I ISO 9439 , . , . 5 KS M ISO 14852:2009 4 20 25 ( 1 ) . . . 6 . 6.1 , ( ) DOC 2 mg/L . 6.2 (6.2.1
6、 ). (6.2.2) . 6.2.1 6.2.1.1 A (6.1) 1 000 mL . (KH 2 PO 4 ) 8.5 g (K 2 HPO 4 ) 21.75 g 2 (Na 2 HPO 4 2H 2 O) 33.4 g (NH 4 Cl) 0.5 g pH , pH 7.4 . 6.2.1.2 B 7 (MgSO 4 7H 2 O) 22.5 g (6.1) 1 000 mL . 6.2.1.3 C 2 (CaCl 2 2H 2 O) 36.4 g (6.1) 1 000 mL . 6.2.1.4 D (III) 6(FeCl 3 6H 2 O) 0.25 g (6.1) 1 00
7、0 mL . (HCl) 1 (EDTA) 0.4 g/L 1 . 6.2.1.5 1 L , (6.1) 500 mL 6.2.1.1 A 10 mL, 6.2.1.2, 6.2.1.3, 6.2.1.4 B, C, D 1 mL , (6.1) 1 000 mL . KS M ISO 14852:2009 5 6.2.2 . pH . 2 400 mg/L , 50 mg/L 2 000 mg/L . C/N 40：1 . 6.2.2.1 A (6.1) 1 000 mL . (KH 2 PO 4 ) 37.5 g 2 (Na 2 HPO 4 2H 2 O) 87.3 g (NH 4 Cl
8、) 2.0 g 6.2.2.2 B 7 (MgSO 4 7H 2 O) 22.5 g (6.1) 1 000 mL . 6.2.2.3 C 2 (CaCl 2 2H 2 O) 36.4 g (6.1) , 1 000 mL . 6.2.2.4 D (III) 6(FeCl 3 6H 2 O) 0.25 g (6.1) 1 000 mL (6.2.1.4 ). 6.2.2.5 E ( , ) (25 %, 7.7 mol/L) 10 mL . ZnCl 270 mg, MnCl 2 4H 2 O 100 mg, H 3 BO 36 mg, CoCl 2 6H 2 O 190 mg, CuCl 2
9、 2H 2 O 3 mg, NiCl 2 6H 2 O 240 mg, Na 2 MoO 4 2H 2 O 36 mg, Na 2 WO 4 2H 2 O 33 mg Na 2 SeO 3 5H 2 O 26 mg (6.1) 1 000 mL . 6.2.2.6 F (, ) 100 mL (6.1) 0.6 mg, 2.0 mg, p- 2.0 mg, 1.0 mg, 10.0 mg, 5.0 mg, 2.0 mg, 5.0 mg, DL-6,8- 5.0 mg 2 1.0 mg . 15 mg (6.1) 100 mL . (membrane filters) (7.6 ). E F ,
10、 , , . 1 mL . 6.2.2.7 KS M ISO 14852:2009 6 1 L 800 mL (6.1) 6.2.2.1 A 100 mL, 6.2.2.2, 6.2.2.3, 6.2.2.4 B, C, D 1 mL( E F ) , (6.1) 1 000 mL pH . pH , pH 7.00.2 . 6.3 (Na 4 P 2 O 7 ) 2.66 g (6.1) 1 000 mL . 7 , . . 7.1 , , ( ). ( ) . 7.2 , 50 1 00 mL/min 10 % ( A ). 7.3 , . , CO 2 DIC ( B ) 7.4 (TO
11、C) (DOC), (KS I ISO 8245 ) 7.5 , 7.6 , ( 0.45 m) 7.7 pH , pH 7.8 , 8 8.1 . TOC ( , KS I ISO 8245 TOC ) ThCO 2 . TOC 100 mg/L . . (6.2.2) , TOC 2 000 mg/L , C/N 40：1 . . KS M ISO 14852:2009 7 , , . . . , . 250 m . , . . , ( ). , , , , (ASTM D 353691 1 ) . , . , . KS I ISO 10634 . 8.2 ( , - -) . TOC,
12、. ( ) . 8.3 . , . , . , . (flora) 8.3.1 8.3.2 . , . (5. ). (CFU) . 10 3 10 6CFU/ mL . 8.3.1 ( 72 ). ( , KS I ISO 11923 3 ). . 30 1 000 mg/L . 1 ( C ), KS M ISO 14852:2009 8 30 mg/L . . 500 mL 2 . 30 . , 15 %(V/V) . . 2 , . . , ( , x %). (KS I ISO 15462 ) ( , ) . 8.3.2 10 g 100 mL(6.2.1 6.2.2 ) (6.3)
13、 30 . , 15 %(V/V) . , . . , ( , , 50 ). 8.4 . a) ( F T ) 2 b) ( F B ) 2 c) ( F C ) 1 , . d) ( F S ) 1. F S (II)(HgCl 2 ) 10 g/L 5 mL/L . . e) ( F N ) 1 f) ( F I ) 1. C/N 40：1 . . 1 (6.2) (8.3 ) . KS M ISO 14852:2009 9 1 F T F T F B F B F C F S () F I () F N () ( A ) . (5. ) , 24 . , . . , . , . ( C
14、), DOC . . 1 (8.1), (8.2) , . 50 100 mL/min(1 12 ) . ( B ). () , . 6 . . pH , 1 mL . 24 , (F T , F C , F B , ) . 9 9.1 9.1.1 (1) mg . ThCO 2 mX C 12 44 (1) KS M ISO 14852:2009 10 m ： (mg) X C ： 44 12 ： F I . 9.1.2 (%) (2) F T D t . 100 ThCO ) CO ( ) CO ( 2 B 2 T 2 t D (2) (CO 2 ) T ： t F T (mg) (CO 2 ) B ： t F B (mg) ThCO 2 ： (mg) . (F C ) (%) . , F I (%), F S F N . , ( C ). 9.2 . 2 . (%) . . , . (wettability) , . . 10 . KS M ISO 14852:2009 11 a) ( F C ) 60 % . b) F B
