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6、recision) . KS J ISO 24276 3.3.7 2 (self-to-self test) RNA 2 3.4 3.4.1 (microarray scanner) DNA 3.4.2 (scanning) DNA 3.4.3 (signal) DNA 3.4.4 (microarray scanner image) 3.4.5 (image analysis) 3.4.6 (normalization) , ( ) 3.4.7 (spot quality control) 4 DNA , . KS P 1017:2012 5 (1) RNA (2) , 5 DNA 5.1
7、 5.1.1 KS J ISO 24276, 6.4 . RNA , a), b) . a) b) (RNase) . . . . ( ) ( ) 1 KS P 1017:2012 6 5.1.2 KS J ISO 24276, 6.6 . . . RNA , . (buffer) . . 5.1.3 KS J ISO 24276, 6.5 , RNA ( , ) RNA . 5.2 5.2.1 DNA . . , DNA . KS P 1017:2012 7 A ( ) . A.1 Cy5 Cyanine 5 5 Cy3 Cyanine 3 3 QC Quality Control UV
8、Ultraviolet DB Database cDNA Complementary DNA CV Coefficient of variance PCR Polymerase Chain Reaction RNase Ribonuclease DNase Deoxyribonuclease cRNA Complementary RNA ssDNA Salmon sperm DNA MML-V Moloney Murine Leukemia Virus KS P 1017:2012 8 1 Sambrook J, Russell DW. Molecular Cloning A laborato
9、ry manual. New York : Cold Spring Harbor Laboratory Press. 2001 2 Schneider J, Buness A, Huber W, Volz J, Kioschis P, Hafner M, Poustka A, Sultmann H. Systematic analysis of T7 RNA polymerase base in vitro linear RNA amplification for use in microarray experiments. BMC Genomics, 2004, vol. 5, p. 29
10、3 Schena M. DNA Microarrays A practical approach. Oxford University Press. 1999 4 Wang X, Ghosh S, Guo SW. Quantitative quality control in microarray image processing and data acquisition. Nucleic Acids Res., 2001, vol. 29, no. 15, p. e75 5 Call DR, Chandler DP, Brockman F. Fabrication of DNA microa
11、rrays using unmodified oligonucleotide probes. Biotechniques, 2001, vol. 30, no. 2, p. 368 372, 374, 376 6 Huang Z, Sanders JC, Dunsmor C, Ahmadzadeh H, Landers JP. A method for UV-bonding in the fabrication of glass electrophoretic microchips. Electrophoresis, 2001, vol. 22, no. 18, p. 3924 3929 7
12、Xiang CC, Brownstein MJ. Fabrication of cDNA microarrays. Methods Mol Biol., 2003, vol. 224, p. 1 7 DNA 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS P 1017:2012KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS The technology for gene expression analysis using DNA microarrays General requirements and definitions ICS 11.040.00
