1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS T ISO 14853 KS T ISO 14853:2012 2012 11 21 http:/www.kats.go.krKS T ISO 14853:2012 : ( ) ( ) 3 ( ) : (http:/www.standard.go.kr) ： ：2012 11 21 2012-0627 ： ： ( 02-509-7274) (http:/www.kats.go.kr). 10 5 , . KS T ISO 14853:2012 i ii 1 1 2 1 3 .1 4 3 5 .3 6 6
2、7 6 8 .10 9 .12 10 .12 A( ) .14 B( ) 15 C( ) 17 D( ) 18 E( ) 20 F( ) (ThCo2) (ThCH4) 21 G( ) 22 H( ) .25 26 KS T ISO 14853:2012 27 KS T ISO 14853:2012 ii 2005 1 ISO 14853, Plastics Determination of the ultimate anaerobic biodegradation of plastic materials in an aqueous system Method by measurement
3、of biogas production , . KS T ISO 14853:2012 Plastics Determination of the ultimate anaerobic biodegradation of plastic materials in an aqueous system Method by measurement of biogas production , , . , . / . 1 . . 60 (2530 ) . . . / , , (inoculums) . (： KS I ISO 13641 ) . . 2 . . ( ) . KS I ISO 8245
4、, (DOC) (TOC) KS I ISO 13641( ), 3 . KS T ISO 14853:2012 2 3.1 (ultimate anaerobic biodegradation) , , (無機化 ) 3.2 1 (primary anaerobic biodegradation) 3.3 (digested sludge) (dewaterability) 35 . 3.4 (concentration of suspended solids in digested sludge) 105 3.5 (dissolved organic carbon) DOC , , 15
5、40 000 m s 2 0.2 m 0.45 m 3.6 (inorganic carbon) IC 3.7 (total dry solids) 105 3.8 (theoretical amount of evolved biogas) Thbiogas (CH4+CO2) 3.9 (theoretical amount of evolved carbon dioxide) ThCO2 KS T ISO 14853:2012 3 3.10 (theoretical amount of evolved methane) ThCH4 3.11 (lag phase, lag period)
6、, 10 % ( ) 3.12 (plateau phase) ( ) 3.13 (biodegradation phase) 90 % ( ) 3.14 (maximum level of biodegradation) , 4 . 20 mg/L 200 mg/L (OC) (352) 60 . (IC) 1 g/L 3 g/L . (CO2) (CH4) ( .) . CO2 . (IC) . IC . IC . . , 1 . . ( G ) . 5 5.1 , 2 mg/L DOC . 5.2 , . . KS T ISO 14853:2012 4 Anhydrous potassi
7、um dihydrogen phosphate KH2PO4 0.27 gDisodium hydrogen phosphate dodecahydrate Na2HPO4 12H2O 1.12 g Ammonium chloride NH4Cl 0.53 Calcium chloride dihydrate CaCl2 2H2O 0.075 g Magnesium chloride hexahydrate MgCl2 6H2O 0.10 Iron(II) chloride tetrahydrate FeCl2 4H2O 0.02 g Resazurin (oxygen indicator)
8、0.001 g Disodium sulfide( ) Na2S 9H2O 0.1 g Stock solution of trace elements( ) 10 mL Vitamin solution No. 1 0.5 mL Stock solutions of vitamins( ) Vitamin solution No. 2 0.5 mL (5.1)( ) 1 L , pH 70.2 . , 20 . , . , (sodium dithionite) . 10 mg/L . 5.3 - ( ) , . Manganese chloride tetrahydrate MnCl2 4
9、H2O 0.05 g Boric acid H3BO30.005 Zinc chloride ZnCl20.005 Copper chloride CuCl20.003 g Disodium molybdate dihydrate Na2MoO4 2H2O 0.001 Cobalt chloride hexahydrate CoCl2 6H2O 0.1 g Nickel chloride hexahydrate NiCl2 6H2O 0.01 Disodium selenite Na2SeO30.005 g Disodium tungstate Na2WO4 2H2O 0.002 (5.1)(
10、 ) 1 L 1 L 10 mL . 5.4 ( ) 5.4.1 1 KS T ISO 14853:2012 5 4-Aminobenzoic acid 40 mg D-Biotin 10 mg (5.1) 500 mL . D-Pantothenic acid, calcium salt 50 mg Pyridoxamine dihydrochloride 150 mg Thiamine dichloride 100 mg ( 0.45 m) 4 . 1 L 0.5 mL . 5.4.2 2 Cyanocobalamin( B12) 10 mg 100 mL (5.1). ( 0.45 m)
11、 4 . 1 L 0.5 mL . 5.5 NaCl 200 g 1 000 mL (5.1) 5 g . bromophenol blue methyl orange . 5.6 20 mg/L 200 mg/L . ( ) . 200 mg/L . , - . 5.7 , , poly-hydroxybutyrate, ( ) 400 . , , , . . 5.8 ( ) KS T ISO 14853:2012 6 (5.2) 5.6 5.7 . 6 6.1 . 6.1.1 , (352) 6.1.2 , 1 mg/L 200 mg/L . 6.2 (manometric) 6.2.1
12、, 0.1 L 1 L , 2 000 hPa(： A ) . 10 %30 % . , 10 % , , 30 % . (serum bottle) . 6.2.2 , , 3 . . . 6.3 6.3.1 (： ), 0.1 L 1 L 250 mL 300 mL . , 10 %20 % . ( B ) ( 5.5) . . 7 7.1 ( ) . 7.2 . . . 1 cm . , KS T ISO 14853:2012 7 . . . (blanks) . (352) 7 . 5 . . , 5 mg/L 20 mg/L OC . . . 7.3 IC 20 mg/L . IC
13、, . , (5.2) (3.7 ) . 3 g/L 1 g/L . (： ). 7.4 3 FT 3 FB FP( ) . FI (inhibition control) ( 1 ). . 7.3 1 g/L 3 g/L . (5.6) (5.7) . OC 100 mg/L . , 1 20 mg/L OC . . (5.1) . ( ) , IC . 70.2 . . 1 , . ( ) . VL VH (6.2.1 ). VL VH(8. ) . , (5.2) . (6.1.1) . KS T ISO 14853:2012 8 1 () FT1 + + FT2 + + FT3 + +
14、 FB1 FB2 + FB3 FP + + + + FI ( ) + + + 7.5 7.5.1 (352) 7.5.2 ( A ) (352) 1 , 0 . , . . 24 48 . . . . . Hectopascal . . . . , . , . . 7.5.3 ( B ) KS T ISO 14853:2012 9 ( ) . (352) 1 , septum 0 . . . . , ( B ). , ( ). . 7.6 60 . (3.12 ) . , . 90 . 7.7 , (IC)(mg/L) . ( ). IC . , . . , 4 2 . , , IC . ,
15、IC . (7.5 ). (： 1 %) 8.2 (3) VH . 7.8 1 (7.4 ) . (VH) (VL) . KS T ISO 14853:2012 10 8 8.1 1 1 12 g . (1) , nm = 00012 (1) m ： 12 000 ： n ： 8.2 (2) n . RTpVn = (2) n ： p ： pascal V ： m3 R ： (8 314 J/mol K) T ： kelvin (3) ( ) . RTVpm)(1.000012Hh=(3) mh ： (mg) VH ： (L) p ： hectopascals 0.1 ： hectopascals , m3 12 000 ： (mg) 35 (308 K) , (4) . )(468.0HhVpm = (4) p(hectopascals) . (3.11 ) ( C ). 8.3 KS T ISO 14853:2012 11 (2.1), (3.1) (4.1) n mh : RTvppnw=)(2.1) RTVppmw Hh)(1.000012 = (3.1) Hh)62.5(468.0 Vpm = (4.1) pw ： (hPa)( E ) p
