1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22074-7 SANS 5334:2008Edition 5SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the common housefly (Musca domestica (L.
4、) Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5334:2008 Edition 5 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Committee SABS
5、 SC 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in December 2008. This document supersedes SABS SM 334:1988 (third revi
6、sion). SANS 5334:2008 Edition 5 1 Pesticides Rearing and handling of the common housefly (Musca domestica (L.) 1 Scope This standard specifies a method for the rearing and handling of the common housefly (Musca domestica (L.). 2 Normative references The following referenced documents are indispensab
7、le for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standards can be obtained from the SABS S
8、tandards Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. SANS 3310-1/ISO 3310-1, Test sieves Technical requirements and testing Part 1: Test sieves of metal wire cloth. 3 Rearing conditions Maintain the rearing conditions prescribed in S
9、ANS 5808, but illuminate the room housing the adult flies by means of fluorescent daylight units that maintain a light intensity of approximately 25 lux. 4 Rearing equipment 4.1 Brush, soft hairbrush. 4.2 Feeding dishes for adults, disposable plastics dishes of approximate diameter 100 mm and depth
10、65 mm. 4.3 Graduated 10 mL glass centrifuge tube. 4.4 Breeding cage for houseflies, as described in SANS 5808. 4.5 Rearing containers, type A rearing containers as described in SANS 5808. 4.6 Rearing container lids 4.6.1 Type A rearing container lids as described in SANS 5808. 4.6.2 Type B rearing c
11、ontainer lids as described in SANS 5808. SANS 5334:2008 Edition 5 2 4.7 Sieves 4.7.1 A woven wirecloth sieve of nominal aperture size in the range 1,7 mm to 2,0 mm, and that complies with the relevant requirements of SANS 3310-1. 4.7.2 Type B sieve as described in SANS 5808. 5 Rearing media 5.1 Feed
12、ing media for adults 5.1.1 Use the following media in separate dishes (see 4.2), filling each dish to approximately 10 mm below the rim: a) brown sugar; and b) full-cream milk powder. 5.1.2 Stopper the glass tubes (that fit the rings of a breeding cage (see 4.4) and then, through the hole near the e
13、nd, fill the tubes with water. So insert a wick made of cotton wool through the hole in each of the tubes that the wick protrudes only slightly through the hole. So insert the two assemblies through the rings in the cage that the holes with the wicks face upwards. 5.2 Oviposition medium and shelter
14、Moisten a layer of cotton wool (contained in a Petri dish) with a mixture of 80 g of full-cream milk powder, 10 g of malt extract and 5 g of brewers yeast powder, suspended in 750 mL of water. Use a second smaller piece of cotton wool moistened with the same suspension to fashion a half-dome on top
15、of the first piece of cotton wool in the Petri dish. (This serves as a necessary shelter for oviposition.) 5.3 Rearing medium for larvae Sift approximately 2,5 kg of digestive bran through the sieve (see 4.7.1) and retain the coarse fraction. Mix together 2 kg of the coarse digestive bran, 300 g of
16、full-cream milk powder, 20 g of brewers yeast powder and 6 g sodium methyl hydroxybenzoate in a rearing container (see 4.5). Add to the mixture 3 280 mL of lukewarm water (approximately 40 C) and mix to obtain a loose-textured product. NOTE 1 Unsifted digestive bran usually contains fine particles t
17、hat, when mixed with water, form a paste instead of a mixture with a loose texture. The use of a pasty mixture has a detrimental effect on the development of the pupae as it usually results in the death of the pupae, and in fungal growth on the mixture. NOTE 2 The quantity of the rearing medium give
18、n in 5.3 is sufficient for one rearing container of the size and dimensions given in 4.5 as the depth of the rearing medium in the container is important. When using smaller containers than those given in 4.5, adjust the volume of the rearing medium and the volume of eggs accordingly. 6 Procedure 6.
19、1 Place approximately 2 000 (400 mL) of the common housefly pupae (see 6.7) and the feeding media for adults (see 5.1) in a breeding cage (see 4.4). Replace the water in the tubes with fresh water every 3 d or as necessary (see 5.1.2). SANS 5334:2008 Edition 5 3 6.2 After a period of 5 d to 7 d afte
20、r emergence of the flies, place a Petri dish containing freshly made oviposition medium (see 5.2) in the cage and leave for approximately 2 h. 6.3 Remove from the cage the Petri dish containing the eggs, moisten the oviposition medium and eggs with water and gently transfer the eggs directly into th
21、e graduated centrifuge tube (see 4.3) using the brush (see 4.1). 6.4 Add approximately 5 mL of distilled water to the centrifuge tube, shake gently to separate the eggs from one another, and then allow them to settle to the bottom of the tube. Note and record the volume of the eggs, shake the centri
22、fuge tube, and so decant and discard the excess eggs that a volume of 1 mL of eggs remains in the tube (equivalent to approximately 8 000 eggs). 6.5 Pour, by rinsing the tube with water, the remaining eggs onto the surface of the freshly prepared rearing medium in the rearing container and cover wit
23、h a thin layer of the medium. Cover the rearing container with a lid (see 4.6.1). 6.6 After 3 d, thoroughly mix the contents of the rearing container and change the lid (see 4.6.2). 6.7 Pupae will form in the upper 25 mm to 40 mm of the rearing medium after a period of 9 d to 11 d after the eggs have been added. 6.8 Remove the pupae from the rearing medium by gently sifting the upper layer of the rearing medium using the sieve assembly specified in 4.7.2. 6.9 Use the procedure described in 6.1 to 6.8 (inclusive) to rear new colonies from a given colony. SABS
