1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22314-4 SANS 5469:2009Edition 2SOUTH AFRICAN NATIONAL STANDARD Soil burial Published by SABS Standards Division 1 Dr Lategan Road Groenklo
4、of Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5469:2009 Edition 2 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Committee SABS SC 1028G, Pesticides Resistance to fungal attack, in accordance
5、with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in June 2009. This document supersedes SABS SM 469:1967 (first edition). SANS 5469:2009 Edition 2 1 Contents Page Foreword 1 Scope 3 2 Apparatus 3 3 Test soil . 3 4 Contro
6、l specimen 3 5 Preparation of test specimens 4 6 Procedure 4 7 Evaluation 4 SANS 5469:2009 Edition 2 2 This page is intentionally left blank SANS 5469:2009 Edition 2 3 Soil burial 1 Scope This standard specifies a method for the soil burial of test specimens in order to determine the effect of micro
7、biological activity on these specimens. 2 Apparatus 2.1 Corrosion-resistant soil container, approximately 50,8 cm in length and approximately 20,32 cm in width and with a depth of at least 12,7 cm. 2.2 Glass sheet or other suitable lid, to cover the soil container. 2.3 Sieve, with a 0,635 cm mesh sc
8、reen. 2.4 Means for maintaining the test soil at a constant temperature of 28 C 2 C, and with an adequate moisture content. 3 Test soil 3.1 Type A well-manured, fertile garden soil without a high clay content, rich in microbial life which decomposes cellulose, and capable of completely deteriorating
9、 the control specimen in 14 d. 3.2 Preparation of soil 3.2.1 Bring the moisture content of the soil to within the optimum limits by gradually adding water. NOTE The optimum moisture content of suitable soils is about 65 % to 70 % of their moisture retaining capacity, or 25 % to 30 % of their oven-dr
10、y weight. 3.2.2 Mix the water with the soil in such a manner as to avoid puddling. 3.2.3 Allow the soil to stand for 24 h then sift it through the sieve. 4 Control specimen A specimen which has in no way been proofed against microbial or fungal attack, but which is otherwise similar to the test spec
11、imens. SANS 5469:2009 Edition 2 4 5 Preparation of test specimens 5.1 Prepare test specimens of suitable dimensions to allow for subsequent tests and of shape that will allow uniform contact with the test soil. When relevant, subject the test specimens to the appropriate pre-treatment specified in t
12、he relevant standard for the required bacteria. 5.2 The quantity and dimensions of the test specimens shall be as specified in the relevant standard for the required bacteria. 6 Procedure 6.1 Submerge the specimens for 1 h in distilled water that contains 0,05 % of a non-ionic wetting agent. 6.2 Lay
13、 part of the test soil at the bottom of the container so as to form a 2,54 cm thick bed. 6.3 Place the test specimens and the control specimen flat on the soil bed, then cover them with a 2,54 cm thick layer of test soil. Cover the container (see 2.2). NOTE In cases where a breaking strength or othe
14、r test is to be used for subsequent evaluation, it is advisable not to cover areas which are later to be inserted in the jaws of a tensile strength testing machine or used for similar anchoring purposes. 6.4 Ensure that the correct moisture content and the correct temperature of the test soil are ma
15、intained throughout the period of incubation, i.e., unless otherwise specified, a period of 14 d. 6.5 On completion of the incubation period, remove the specimens and gently wash off all adhering soil particles. 6.6 Dry the specimens in air at room temperature and examine the control specimen to det
16、ermine whether the degree of microbiological activity was satisfactory. In cases where this is not evident, discard the specimens and repeat the test on a new set of test specimens with freshly prepared soil. 7 Evaluation 7.1 Visual examination Examine the test specimens for any apparent deteriorati
17、on and report the findings. 7.2 Other tests Determine the breaking strength or carry out other tests (or do both) in accordance with the method(s) specified in the relevant standard for the required bacteria. SABS SABS Standards Division The objective of the SABS Standards Division is to develop, pr
18、omote and maintain South African National Standards. This objective is incorporated in the Standards Act, 2008 (Act No. 8 of 2008). Amendments and Revisions South African National Standards are updated by amendment or revision. Users of South African National Standards should ensure that they posses
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