1、BSI Standards Publication BS ISO 8896:2016 Essential oil of caraway (C C Ca a ar r ru u um m m c c ca a ar r rv v vi i i L.)BS ISO 8896:2016 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 8896:2016. The UK participation in its preparation was entrusted to Te
2、chnical Committee AW/54, Essential oils. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standard
3、s Institution 2016. Published by BSI Standards Limited 2016 ISBN 978 0 580 87122 1 ICS 71.100.60 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 April 2016.
4、Amendments/corrigenda issued since publication Date T e x t a f f e c t e dBS ISO 8896:2016 ISO 2016 Essential oil of caraway (Carum carvi L.) Huile essentielle de carvi (Carum carvi L.) INTERNATIONAL STANDARD ISO 8896 Second edition 2016-04-01 Reference number ISO 8896:2016(E)BS ISO 8896:2016ISO 88
5、96:2016(E)ii ISO 2016 All rights reserved COPYRIGHT PROTECTED DOCUMENT ISO 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying
6、, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01
7、 11 Fax +41 22 749 09 47 copyrightiso.org www.iso.orgBS ISO 8896:2016ISO 8896:2016(E)Foreword iv 1 Scope . 1 2 Normative references 1 3 T erms and definitions . 1 4 Requirements 1 4.1 Appearance 1 4.2 Colour 1 4.3 Odour . 2 4.4 Relative density at 20 C . 2 4.5 Refractive index at 20 C . 2 4.6 Optica
8、l rotation at 20 C 2 4.7 Acid value. 2 4.8 Miscibility in ethanol 80 % (volume fraction) at 20 C 2 4.9 Chromatographic profile 2 5 Flashpoint 2 6 Sampling 3 7 Test methods . 3 7.1 Relative density at 20 C . 3 7.2 Refractive index at 20 C . 3 7.3 Optical rotation at 20 C 3 7.4 Acid value. 3 7.5 Misci
9、bility in ethanol 80 % (volume fraction) at 20 C 3 7.6 Chromatographic profile 3 8 Packaging, labelling, marking and storage . 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatographyof the essential oil of caraway (Carum carvi L.) . 4 Annex B (informative) Flashpoint . 6
10、Bibliography 7 ISO 2016 All rights reserved iii Contents PageBS ISO 8896:2016ISO 8896:2016(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carrie
11、d out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. IS
12、O collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different
13、 approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives). Attention is drawn to the possibility that some of the elements of this document may be
14、 the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).
15、 Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles
16、in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information The committee responsible for this document is ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 8896:1987), which has been technically revised.iv ISO 2016 Al
17、l rights reservedBS ISO 8896:2016INTERNATIONAL ST ANDARD ISO 8896:2016(E) Essential oil of caraway (Carum carvi L.) 1 Scope This International Standard specifies certain characteristics of the essential oil of caraway (Carum carvi L.), in order to facilitate assessment of its quality. 2 Normative re
18、ferences The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies
19、. ISO/TS 210, Essential oils General rules for packaging, conditioning and storage ISO/TS 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential o
20、ils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024 (all parts), Essential oils General guidance on chromatographic profiles 3 T erm
21、s a nd definiti ons For the purposes of this document, the following terms and definitions apply. 3.1 essential oil of Caraway essential oil obtained by steam distillation from the dried, ripe fruit of caraway (Carum carvi L.) of the Apiaceae family Note 1 to entry: For information on the CAS number
22、, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear mobile liquid. 4.2 Colour Colourless to pale yellow. ISO 2016 All rights reserved 1BS ISO 8896:2016ISO 8896:2016(E) 4.3 Odour Fresh, herbaceous and spicy. 4.4 Relative density at 20 C, d Minimum: 0,900 Maximum: 0,920 4.5 Refractive index at 20
23、C Minimum: 1,484 Maximum: 1,490 4.6 Optical rotation at 20 C Between +67 and +80. 4.7 Acid value Maximum: 1,0 4.8 Miscibility in ethanol 80 % (volume fraction) at 20 C It shall not be necessary to use more than 3 volumes of ethanol 80 % (volume fraction) to obtain a clear solution with 1 volume of e
24、ssential oil. Sometimes opalescence can arise on continuing the addition of ethanol. 4.9 Chr omat ogr aphic p r ofile Carry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representative and characteristic components shown in Table 1. The propo
25、rtions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. T a b l e 1 C h r o m a t o g r a p h i c p r o f i l e Component Min. Max. % % Myrcene 0,2 0,7 Limonene 33,0 45,0 cis-Dihydrocarvone 0,1 1,5 tran
26、s-Carveol traces a 0,5 cis-Carveol 0,2 0,5 Carvone 50,0 63,0 atraces: 0,01 %. NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 5 Flashpoint Information on the flashpoint is given in Annex B.2 ISO 2016 All rights reservedBS ISO 8896:20
27、16ISO 8896:2016(E) 6 Sampling Sampling shall be performed in accordance with ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 7 Test methods 7.1 Relative density at 20 C, d Determine the
28、 relative density in accordance with ISO 279. 7.2 Refractive index at 20 C Determine the refractive index in accordance with ISO 280. 7.3 Optical rotation at 20 C Determine the optical rotation in accordance with ISO 592. 7.4 Acid value Determine the acid value in accordance with ISO 1242. 7.5 Misci
29、bility in ethanol 80 % (volume fraction) at 20 C Determine the miscibility in ethanol in accordance with ISO 875. 7.6 Chr omat ogr aphic p r ofile Determine the chromatographic profile in accordance with ISO 11024 (all parts). 8 Packaging, labelling, marking and storage These items shall be in accor
30、dance with ISO/TS 210 and ISO/TS 211. ISO 2016 All rights reserved 3BS ISO 8896:2016ISO 8896:2016(E) Annex A (informative) Typical chromatograms of the analysis by gas chromatographyof the essential oil of caraway (Carum carvi L.)P eak identification Operating conditions 1 Myrcene Column: fused capi
31、llary silica, 30 m length, 0,25 mm internal diameter 2 Limonene Stationary phase: SPB TM1 (SE-30) a 3 cis-Dihydrocarvone Film thickness: 0,25 m 4 Carvone Oven temperature: programming temperature from 50 C to 180 C at a rate of 3 C/min 5 trans-Carveol and 180 C to 220 C at a rate of 10 C/min 6 cis-C
32、arveol Injector temperature: 260 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: helium Volume injected: 1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/50 aSPB TM1 (SE-30) is an example of a suitable product available commercially. This information is given for the con
33、venience of users of this document and does not constitute an endorsement by ISO of this product. Figure A.1 Typical chromatogram taken on an apolar column4 ISO 2016 All rights reservedBS ISO 8896:2016ISO 8896:2016(E)P eak identification Operating conditions 1 Myrcene Column: fused capillary silica,
34、 30 m length, 0,25 mm internal diameter 2 Limonene Stationary phase: Supelco Wax TM10 a 3 cis-Dihydrocarvone Film thickness: 0,25 m 4 Carvone Oven temperature: programming temperature from 50 C to 180 C at a rate of 3 C/min 5 trans-Carveol and 180 C to 220 C at a rate of 10 C/min 6 cis-Carveol Injec
35、tor temperature: 260 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: helium Volume injected: 1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/50 aSupelco Wax TM10 is an example of a suitable product available commercially. This information is given for the convenience of
36、 users of this document and does not constitute an endorsement by ISO of this product. Figure A.2 Typical chromatogram taken on a polar column ISO 2016 All rights reserved 5BS ISO 8896:2016ISO 8896:2016(E) Annex B (informative) Flashpoint B.1 General information For safety reasons, transport compani
37、es, insurance companies, and people in charge of safety services require information on the flash points of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single app
38、aratus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high priced essential oils; as there are several different types of equipment which can be used fo
39、r the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties. The equipment with which this value
40、 was obtained shall be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essential oil of caraway (Carum carvi L.) The mean value is +57 C. NOTE Obtained with “closed cup” 1)equipment. 1) Equipment available commercially. This information is given for the convenience of use
41、rs of this document and does not constitute an endorsement by ISO of this product.6 ISO 2016 All rights reservedBS ISO 8896:2016ISO 8896:2016(E) Bibliography 1 ISO 3218, Essential oils Principles of nomenclature 2 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint 3 ISO
42、/TR 21092, Essential oils Characterization 4 Gildemeister E., & Hoffmann Fr. Die therischen le Vol. VII 649-660 pp. ( 1961) Akademie- Verlag, Berlin 5 Lawrence M. Progress in essential oils. Perfum. Flavor. 1980, 5 (4) pp. 616 ISO 2016 All rights reserved 7BS ISO 8896:2016ISO 8896:2016(E) ISO 2016 A
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