1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN ISO 13132:2011Laboratory glassware Petridishes (ISO 13132:2011)BS EN ISO 13132:2011 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN IS
2、O13132:2011.The UK participation in its preparation was entrusted to TechnicalCommittee LBI/36, Laboratory glassware and related apparatus.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessarypr
3、ovisions of a contract. Users are responsible for its correctapplication. BSI 2011ISBN 978 0 580 68043 4ICS 71.040.20Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee o
4、n 31 August 2011.Amendments issued since publicationDate Text affectedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 13132 July 2011 ICS 71.040.20; 11.100.01 English Version Laboratory glassware - Petri dishes (ISO 13132:2011) Verrerie de laboratoire - Botes de Petri (ISO 13132:2011) Labor
5、gerte aus Glas - Petrischalen (ISO 13132:2011) This European Standard was approved by CEN on 14 July 2011. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterati
6、on. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by
7、 translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
8、Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCH
9、ES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 13132:2011: EBS EN ISO 13132:2011EN ISO 13132:2011 (E) 3 Foreword This document (EN ISO 13132:2011)
10、 has been prepared by Technical Committee ISO/TC 48 “Laboratory equipment“ in collaboration with Technical Committee CEN/TC 332 “Laboratory equipment” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identic
11、al text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at the latest by January 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held re
12、sponsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Fi
13、nland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 13132:2011 has been approved by CEN as a E
14、N ISO 13132:2011 without any modification. BS EN ISO 13132:2011ISO 13132:2011(E) ISO 2011 All rights reserved iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards
15、 is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take p
16、art in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to
17、 prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility t
18、hat some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 13132 was prepared by Technical Committee ISO/TC 48, Laboratory equipment, Subcommittee SC 6, Glass and plastics ware including volumetr
19、ic instruments. BS EN ISO 13132:2011BS EN ISO 13132:2011INTERNATIONAL STANDARD ISO 13132:2011(E) ISO 2011 All rights reserved 1Laboratory glassware Petri dishes 1 Scope This International Standard specifies requirements and tests for glass Petri dishes intended for general laboratory purposes and mi
20、crobiological work. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 71
21、9, Glass Hydrolytic resistance of glass grains at 98 C Method of test and classification 3 Types Two types of Petri dishes are specified in this International Standard. Type 1 Thick-walled Petri dishes Type 2 Thin-walled Petri dishes 4 Nominal sizes and series 4.1 Nominal sizes Petri dishes shall ha
22、ve the following nominal sizes. 50, 60, 80, 100, 120, 150 and 200 millimetres NOTE For Series B, nominal sizes refer to the external diameter of the bottom dish. 4.2 Series Petri dishes shall comply with the following dimensional requirements. Series A Dimensions in accordance with Table 1 Series B
23、Dimensions in accordance with Table 2 5 Designation Petri dishes in accordance with this International Standard shall be designated by their nominal size followed by the type and the series. BS EN ISO 13132:2011ISO 13132:2011(E) 2 ISO 2011 All rights reservedDesignation of a thick-walled Petri dish
24、with nominal size of 120 mm, Type 1, Series A: Petri dish ISO 13132 120 20 1A If bottom dishes (2) and top dishes (1) are ordered separately, the following designations shall be used. Designation of a thick-walled bottom dish (2) of nominal size 120 mm, Type 1, Series A: Petri bottom dish ISO 13132
25、120 20 1A 2 Designation of a thick-walled top dish (1) of nominal size 120 mm, Type 1, Series A: Petri top dish ISO 13132 120 20 1A 1 6 Material Petri dishes shall be manufactured from transparent glass of hydrolytic resistance Class HBG 1, Class HBG 2 or Class HGB 3 in accordance with ISO 719. The
26、glass shall be reasonably free from residual strain and from defects which might impair safety, durability or appearance such as mould marks and waviness. The glass shall not have any pronounced tint. 7 Dimensions 7.1 Series A Petri dishes Series A Petri dishes shall comply with the dimensions speci
27、fied in Table 1. Key 1 top dish 2 bottom dish Key 1 top dish 2 bottom dish Figure 1 Thick-walled Petri dish Figure 2 Thin-walled Petri dish BS EN ISO 13132:2011ISO 13132:2011(E) ISO 2011 All rights reserved 3Table 1 Dimensions for Series A Petri dishes Dimensions in millimetres Type 1 Thick-walled (
28、see Figure 1) Type 2 Thin-walled (see Figure 2) Nominal size d1d201h1 1 h2 1 s d1 1 d2 1 h1 1,5 h2 1,5 s 0,3 50 12 47 45 9 14 1,3 60 20 1,5055+54 15 20 2,5 56 53 15 20 1,8 80 15 77 74 11 15 1,3 80 20 1,5071+70 15 20 2,5 76 73 15 20 1,8 100 15 15 15 100 20 1,5091+90 13 20 3 96 93 11,5 20 1,8 120 20 1
29、,50111+110 15 20 3,5 116 113 15 20 1,8 150 30 20140+139 15 30 4 146 142 18 27 1,8 200 30 197 193 23 29 1,6 7.2 Series B Petri dishes Series B Petri dishes shall comply with the dimensions specified in Table 2. For any pair of Series B Petri dishes, the difference between the internal diameter of the
30、 top dish and external diameter of the bottom dish shall be between 2 mm to 4 mm. Table 2 Dimensions for Series B Petri dishes Dimensions in millimetres Type 2 Thin-walled (see Figure 2) Nominal size d110+d201h101h210+s min. 50 17 53 50 15 17 1,0 80 17 83 80 15 17 1,0 100 17 103 100 15 17 1,5 150 20
31、 153 150 17 20 1,5 200 20 203 200 20 20 1,5 8 Construction 8.1 Basic requirements Petri dishes shall be regular in shape (see e.g. Figure 1) and smoothly finished. They shall be symmetrical about the axis which shall be perpendicular to the plane of the base. BS EN ISO 13132:2011ISO 13132:2011(E) 4
32、ISO 2011 All rights reserved8.2 Base and side The bases of both the bottom and top dishes shall be, as far as possible, in one plane surface and the sides shall be perpendicular to the base with a maximum deviation of 3. The bases may be given a slight crown to prevent rocking. 8.3 Edge The edges of
33、 the side wall of both bottom and top dishes shall be smoothly ground, fire-polished or clean pressed. The edges shall be in a plane parallel to their bases. If the edges are finished by burn-off process, there shall not be any tear-drop extending above the plane of cut-off in the bottom dish. In th
34、e top dish such protrusion up to 1 mm is permissible. 8.4 Flatness of bottom dishes The inside depth at any two points on the base of the bottom dish shall not vary by more than 1,2 mm when measured in accordance with Annex A. 8.5 Performance requirements Petri dishes shall not show visible decrease
35、 of transparency, opalescence, iridescence, frosting or cracks after testing by the following procedures: minimum 15 min wet sterilization at minimum 120 C; minimum 1 h dry sterilization at minimum 160 C. 9 Marking Petri dishes shall be permanently and legibly marked on its sidewall with the followi
36、ng information: a) manufacturers and/or suppliers name and/or trade mark; b) optionally the number of this International Standard, “ISO 13132”. BS EN ISO 13132:2011ISO 13132:2011(E) ISO 2011 All rights reserved 5Annex A (normative) Test method for flatness The following type test procedure applies f
37、or measurement of flatness of the inner surface of the Petri bottom dish. a) Affix a dial gauge to a stand. b) Place both the measuring equipment and the Petri dish under test on the same flat and firm surface. c) Lower the dial gauge in such a way that the pointer is pressed against the inner surfa
38、ce of the Petri dish so that some deflection is seen on the pointer. d) Then measure with the dial gauge the difference between the deepest and the highest point of the inner surface which should not exceed the value specified in 8.4. This page deliberately left blankThis page deliberately left blan
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