1、BRITISH STANDARD BS EN ISO 8655-4:2002 Piston-operated volumetric apparatus Part 4: Dilutors ICS 17.060 Incorporating Corrigendum December 2008National foreword This British Standard is the UK implementation of EN ISO 8655-4:2002. It is identical with ISO 8655-4:2002, incorporating corrigendum Decem
2、ber 2008. It, together with Parts 2, 3 and 5 of BS EN ISO 8655:2002, supersedes BS 7653-2:1993 which is withdrawn. The start and finish of text introduced or altered by corrigendum is indicated in the text by tags. Text altered by ISO corrigendum December 2008 is indicated in the text by . The UK pa
3、rticipation in its preparation was entrusted by Technical Committee LBI/36, Laboratory glassware and related apparatus, to Subcommittee LBI/36/1, General and volumetric, glass and plastics apparatus. A list of organizations represented on this committee can be obtained on request to its secretary. T
4、his publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunity from legal obligations. BS EN ISO 8655-4:2002 Amendments issued since publication Date Comments 30 June
5、 2009 Implementation of ISO corrigendum December 2008 This British Standard, having been prepared under the direction of the Materials and Chemicals Sector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 22 October 2002 BSI 2009 ISBN
6、 978 0 580 66191 4 /corrigendaEUROPEANSTANDARD NORMEEUROPENNE EUROPISCHENORM ENISO86554 September2002 ICS17.060 Englishversion PistonoperatedvolumetricapparatusPart4:Dilutors(ISO 86554:2002) AppareilsvolumtriquespistonPartie4:Diluteurs(ISO 86554:2002) VolumenmessgertemitHubkolbenTeil4:Dilutoren(ISO
7、86554:2002) ThisEuropeanStandardwasapprovedbyCENon13July2002. CENmembersareboundtocomplywiththeCEN/CENELECInternalRegulationswhichstipulatetheconditionsforgivingthisEuropean Standardthestatusofanationalstandardwithoutanyalteration.Uptodatelistsandbibliographicalreferencesconcerningsuchnational stand
8、ardsmaybeobtainedonapplicationtotheManagementCentreortoanyCENmember. ThisEuropeanStandardexistsinthreeofficialversions(English,French,German).Aversioninanyotherlanguagemadebytranslation undertheresponsibilityofaCENmemberintoitsownlanguageandnotifiedtotheManagementCentrehasthesamestatusastheofficial
9、versions. CENmembersarethenationalstandardsbodiesofAustria,Belgium,CzechRepublic,Denmark,Finland,France,Germany,Greece, Iceland,Ireland,Italy,Luxembourg,Malta,Netherlands,Norway,Portugal,Spain,Sweden,SwitzerlandandUnitedKingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPIS
10、CHESKOMITEEFRNORMUNG ManagementCentre:ruedeStassart,36B1050Brussels 2002CEN Allrightsofexploitationinanyformandbyanymeansreserved worldwideforCENnationalMembers. Ref.No.ENISO86554:2002EINESO6855:42(200E) 2 Foreword Thisdocument(ISO86554:2002)hasbeenpreparedbyTechnicalCommitteeISO/TC48 “Laboratorygla
11、sswareandrelatedapparatus“incollaborationwithTechnicalCommittee CEN/TC332“Laboratoryequipment“,thesecretariatofwhichisheldbyDIN. ThisEuropeanStandardshallbegiventhestatusofanationalstandard,eitherbypublication ofanidenticaltextorbyendorsement,atthelatestbyMarch2003,andconflictingnational standardssh
12、allbewithdrawnatthelatestbyMarch2003. AccordingtotheCEN/CENELECInternalRegulations,thenationalstandardsorganizationsof thefollowingcountriesareboundtoimplementthisEuropeanStandard:Austria,Belgium, CzechRepublic,Denmark,Finland,France,Germany,Greece,Iceland,Ireland,Italy, Luxembourg,Malta,Netherlands
13、,Norway,Portugal,Spain,Sweden,Switzerlandandthe UnitedKingdom. Endorsementnotice ThetextoftheInternationalStandardISO86554:2002hasbeenapprovedbyCENasa EuropeanStandardwithoutanymodifications. NOTENormativereferencestoInternationalStandardsarelistedinAnnexZA(normative). ENISO86554:2002 BS EN ISO 8655
14、-4:2002INTERNATIONAL STANDARD ISO 86554 Firstedition 20020915 Referencenumber ISO 86554:2002(E) Pistonoperatedvolumetricapparatus Part 4: Dilutors Appareilsvolumtriquespiston Partie 4:Diluteurs ENISO86554:2002 BS EN ISO 8655-4:2002ISO 55682:4)E(200 ii ISO 2002 Arllithgrseresvde FDPdicslaimre ThisFDP
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19、OSI 55682002:4)E( ISO 2002 rllAithgsreservde iii Contents Page 1 Scope. 1 2 Normativereferences . 1 3 Termsanddefinitions 1 4 Principleofoperation 2 5 Adjustment 2 6 Metrologicalperformancerequirements . 3 7 Userinformation . 4 8 Marking. 4 Bibliography. 6 iii ENISO86554:2002 BS EN ISO 8655-4:2002IS
20、O 55682:4)E(200 vi ISO 2002 Arllithgrseresvde Foreword ISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(ISO memberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughISOtechnical committees.Eachmemberbodyinterestedinasubjectfor
21、whichatechnicalcommitteehasbeenestablishedhas therighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnongovernmental,in liaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnical Commission(IEC)onallmattersofelectrotechnicalstandardizati
22、on. InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part 3. DraftInternationalStandardsadoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting. PublicationasanInternationalStandardrequiresapprovalbyatleast75 %ofthememberbodiescastingavote. Attention
23、isdrawntothepossibilitythatsomeoftheelementsofthispartofISO 8655maybethesubjectofpatent rights.ISOshallnotbeheldresponsibleforidentifyinganyorallsuchpatentrights. InternationalStandardISO 86554waspreparedbyTechnicalCommitteeISO/TC 48, Laboratoryglasswareand relatedapparatus,SubcommitteeSC 1,Volumetr
24、icinstruments. ISO 8655consistsofthefollowingparts,underthegeneraltitlePistonoperatedvolumetricapparatus : Part 1: Terminology,generalrequirementsanduserrecommendations Part 2: Pistonpipettes Part 3: Pistonburettes Part 4: Dilutors Part 5: Dispensers Part 6: Gravimetricmethodsforthedeterminationofme
25、asurementerror iv ENISO86554:2002 Part 7: Non-gravimetric methods for the assessment of equipment performance BS EN ISO 8655-4:2002OSI 55682002:4)E( ISO 2002 rllAithgsreservde v Introduction ISO 8655addressestheneedsof: suppliers,asabasisforqualitycontrolincluding,whereappropriate,theissuanceofsuppl
26、iersdeclarations; testhousesandotherbodies,asabasisforindependentcertification; usersoftheequipment,toenableroutinecheckingofaccuracy. Thetestsspecifiedshouldbecarriedoutbytrainedpersonnel. v ENISO86554:2002 BS EN ISO 8655-4:2002 blank BS EN ISO 8655-4:2002ANRETNIITOTSLANDNADRA OSI 55682002:4)E( ISO
27、 2002 rllAithgsreservde 1 Pistonoperatedvolumetricapparatus Part 4: Dilutors 1 Scope ThispartofISO 8655specifies metrologicalrequirements, maximumpermissibleerrors, requirementsformarkingand informationtobeprovidedforusers, fordilutorswithasampleuptakecapacity(In)from to andadiluentcapacity(Ex)from
28、to .They aredesignedtodeliverthesampleanddiluenttogetherinmeasuredproportionandmeasuredvolume. 2 Normativereferences Thefollowingnormativedocumentscontainprovisionswhich,throughreferenceinthistext,constituteprovisionsof thispartofISO 8655.Fordatedreferences,subsequentamendmentsto,orrevisionsof,anyof
29、thesepublicationsdo notapply.However,partiestoagreementsbasedonthispartofISO 8655areencouragedtoinvestigatethe possibilityofapplyingthemostrecenteditionsofthenormativedocumentsindicatedbelow.Forundatedreferences, thelatesteditionofthenormativedocumentreferredtoapplies.MembersofISOandIECmaintainregis
30、tersof currentlyvalidInternationalStandards. ISO 3696,Waterforanalyticallaboratoryuse Specificationandtestmethods ISO 86551:2002, Pistonoperatedvolumetricapparatus Part 1:Terminology,generalrequirementsanduser recommendations ISO 86556:2002,Pistonoperatedvolumetricapparatus Part 6:Gravimetricmethods
31、forthedeterminationof measurementerror 3 Termsanddefinitions ForthepurposesofthispartofISO 8655,thetermsanddefinitionsgiveninISO 86551apply. 5l1 0 m l 5 0 l 100 ml 1 ENISO86554:2002 NOTE GeneralrequirementsanddefinitionsoftermsofpistonoperatedvolumetricapparatusaregiveninISO86551. bythesupplier,anal
32、yticalandmeasuringequipmentqualityassurancebytheuser)seeISO 86556oralternativetestmethods. Forsafetyrequirementsofelectricallypoweredpistonpipettes,seeIEC 610101. methodssuchasphotometricandtitrimetricmethodsaregiveninISO 86557. Forallothertests(e.g.qualityassu rance Conformitytesting(typeevaluation
33、)ofpistonoperatedvolumetricapparatusisgiveninISO 86556. Alternativetest BS EN ISO 8655-4:20024 Principleofoperation Adilutorisdesignedtoaspirateaccuratelyameasuredvolumeofasampleliquidandtodeliverittogetherwithan accuratelymeasuredvolumeofdiluent.Dilutorsmaybeoperatedmanually,electrically,pneumatica
34、llyor hydraulicallyandmaybehandheld,bottletopmountingorfreestandingbenchtopapparatus.Theycanalsobe automatedanalyserscomponents.Thedrivecomponentsmaybeintegralwith,ormanuallyseparablefromthe volumetricmeasuringcomponents(changeoverunits). Priortodeliveryofdiluent,thediluentpistonsystemischargedbyasp
35、irationofdiluentfromareservoir.Afterair bubblefreefillingofthesystem,diluentisdrawnintothevolumemeasuringcylinderbythediluentpiston,either directly,viatheuptakeanddeliveryprobe,orindirectly,fromareservoiruntilavolumecontrollinglimitisreached.A measuredvolumeofsampleisthenaspiratedintotheuptakeanddel
36、iveryprobe. Theuptakeofsamplemaybecontrolledbyasecondlimittothemovementofthediluentpiston,oritmayinvolvea second,dedicated,cylinderandpistonwithvalves.Duringdelivery,thesamplevolumeintheuptakeanddelivery probeisexpelled,followedbythemeasuredquantityofdiluent. 5 Adjustment 5.1 Adilutorshallbeadjusted
37、byitssupplierfortheuptakeanddeliveryofitsstatedvolumesandforthestandard referencetemperatureof ,usinggrade 3waterasspecifiedinISO 3696. 5.2 Ifthedilutorisdesignedtohaveitsfactorypresetadjustmentalteredbytheuser,thedesignshallprevent unintentionalreadjustment.Iftheuserreadjuststhedilutor,itshallbecle
38、arlyandunequivocallyindicatedonthe outsideofthedilutorthatreadjustmenthasbeeneffected,forexamplebymeansofamark,abrokenseal,oralabel affixedbytheuser. 5.3 Typetestingoftheadjustmentshallsatisfytheperformancerequirementsspecifiedinclause 6whenthedilutor istestedinaccordancewithISO 86556. Key 1 Volumeo
39、fdiluent 2 Pistonforsampleuptake Figure 1 Schematicdrawingofadilutor 20 C 2 ENISO86554:2002 When a dilutor is required for use in a country which has adopted a standard referencetemperatureof 27 C (the alternative recommended for tropical use), this figure shall be substituted for 20 C. BS EN ISO 86
40、55-4:2002OSI 55682002:4)E( ISO 2002 rllAithgsreservde 3 6 Metrologicalperformancerequirements Theconformitytest(typeevaluation)specifiedinISO 86556evaluatesthetotalsystemofthedilutor,comprisingthe pistonunitsandvalves,drive,uptakeanddeliveryprobeandtubesand,ifapplicable,thechangeoverunit(see clause
41、4). TheconformitytestshallbecarriedoutinaccordancewithISO 86556by thesupplier,priortotheissuanceofasuppliersdeclarationofconformity,or atesthouseorotherauthorizedbody,priortotheissuanceofacertificateofconformity. Forconformitytests,themaximumpermissibleerrorsofTables 1and2shallapply.Ifasuppliersdecl
42、arationora certificateofconformityisissued,itshallbestatedpreciselywhichspecificcomponentshavebeentested.Theuse ofsubstitutetubinginvolvesalterationofthesystemtypetestedandthismayinvalidatethesuppliersorthirdparty certifiedvolumetricperformance. Table 1 Maximumpermissibleerrorsofsampleuptake Nominal
43、volume Maximumpermissiblesystematicerror Maximumpermissiblerandomerror ab c 53 , 00 , 1 520 , 1 10 2,0 0,2 0,8 0,08 20 2,0 0,4 0,8 0,16 50 1,8 0,9 0,4 0,2 100 1,5 1,5 0,2 0,2 200 1,0 2,0 0,2 0,4 500 0,8 4,0 0,2 1,0 1 000 0,6 6,0 0,15 1,5 a Expressedasthedeviationofthemeanofatenfoldmeasurementfromthenominalvolumeorfromtheselectedvolume (see ISO 86556:2002,8.4). b Expressedasthecoefficientofvariationofatenfoldmeasurement(seeISO 86556:2002,8.5) c Expressedastherepeatabilitystandarddeviationofatenfoldmeasurement(
