BS ISO 14461-1-2005 Milk and milk products - Quality control in microbiological laboratories - Analyst performance assessment for colony counts《奶和奶制品 微生物实验室内质量控制 菌落计数用分析性能评定》.pdf
《BS ISO 14461-1-2005 Milk and milk products - Quality control in microbiological laboratories - Analyst performance assessment for colony counts《奶和奶制品 微生物实验室内质量控制 菌落计数用分析性能评定》.pdf》由会员分享,可在线阅读,更多相关《BS ISO 14461-1-2005 Milk and milk products - Quality control in microbiological laboratories - Analyst performance assessment for colony counts《奶和奶制品 微生物实验室内质量控制 菌落计数用分析性能评定》.pdf(44页珍藏版)》请在麦多课文档分享上搜索。
1、BRITISH STANDARD BS ISO 14461-1:2005 Milk and milk products Quality control in microbiological laboratories Part 1: Analyst performance assessment for colony counts ICS 07.100.30 BS ISO 14461-1:2005 This British Standard was published under the authority of the Standards Policy and Strategy Committe
2、e on 13 September 2005 BSI 13 September 2005 ISBN 0 580 46093 2 National foreword This British Standard reproduces verbatim ISO 14461-1:2005 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology, which has the r
3、esponsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “Internatio
4、nal Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British
5、 Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and Europ
6、ean developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the ISO title page, pages ii to vi, pages 1 to 34, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was
7、 last issued. Amendments issued since publication Amd. No. Date Comments Reference numbers ISO 14461-1:2005(E) IDF 169-1:2005(E)INTERNATIONAL STANDARD ISO 14461-1 IDF 169-1 First edition 2005-05-01 Milk and milk products Quality control in microbiological laboratories Part 1: Analyst performance ass
8、essment for colony counts Lait et produits laitiers Contrle de qualit en laboratoires microbiologiques Partie 1: valuation de la performance des analystes effectuant les comptages de colonies BS ISO 14461-1:2005 ii BS ISO 14461-1:2005 iii Contents Page Foreword iv Introduction . vi 1 Scope 1 2 Norma
9、tive references . 1 3 Terms and definitions. 1 4 Principle (see Figure 1) 2 5 Diluent, culture media and reagents . 3 6 Apparatus and glassware. 5 7 Sampling 5 8 Preparation of test sample . 6 8.1 Milk . 6 8.2 Dried milk . 6 9 Procedure. 6 9.1 General . 6 9.2 Number of decimal dilution steps . 6 9.3
10、 Preparation of first decimal dilution . 6 9.4 Preparation of further decimal dilutions. 7 9.5 Melting the medium 7 9.6 Preparation of binary dilutions and inoculation of the medium 7 9.7 Incubation 7 9.8 Randomization and counting of colonies. 8 10 Statistical evaluation 10 10.1 Adequacy of the dat
11、a set 10 10.2 Evaluation of the complete data set (see Figure 2) 10 Annex A (informative) Weighted mean and homogeneity testing of colony counts 27 Annex B (Informative) BASIC programme for calculating the likelihood ratio index G 2 33 Bibliography . 34 iv Foreword ISO (the International Organizatio
12、n for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has
13、 the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Inter
14、national Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publicat
15、ion as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent righ
16、ts. ISO 14461-1IDF 169-1 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.
17、 ISO 14461IDF 169 consists of the following parts, under the general title Milk and milk products Quality control in microbiological laboratories: Part 1: Analyst performance assessment for colony counts Part 2: Determination of the reliability of colony counts of parallel plates and subsequent dilu
18、tion steps BS ISO 14461-1:2005 v Foreword IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National Committee in every member country. Every National Committee has the right to be represented on the IDF Standing Committees carrying out the technical work.
19、 IDF collaborates with ISO and AOAC International in the development of standard methods of analysis and sampling for milk and milk products. Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National Committees for voting. Publication as an Inte
20、rnational Standard requires approval by at least 50 % of the National Committees casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IDF shall not be held responsible for identifying any or all such patent rights. This
21、 part of International Standard ISO 14461-1IDF 169 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC International. It is being published jointly by ISO and IDF and separat
22、ely by AOAC International. All work was carried out by the Joint ISO/IDF/AOAC Action Team, Statistics of analytical data, of the Standing Committee on Quality assurance, statistics of analytical data and sampling, under the aegis of its project leaders, Dr. H. Glaeser (EU) and Prof. Dr. H. Weiss (DE
23、). This edition of ISO 14461-1IDF 169-1, together with ISO 14461-2IDF 169-2, cancels and replaces IDF 169:1994, which has been technically revised. ISO 14461IDF 169 consists of the following parts, under the general title Milk and milk products Quality control in microbiological laboratories: Part 1
24、: Analyst performance assessment for colony counts Part 2: Determination of the reliability of colony counts of parallel plates and subsequent dilution steps BS ISO 14461-1:2005 vi Introduction Every microbiological method consists of several steps that are followed in a specific sequence (sub-sampl
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