1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 19012-2:2013Microscopes Designation ofmicroscope objectivesPart 2: Chromatic correctionBS ISO 19012-2:2013 BRITISH STANDARDNational forewordThis British Standard is the UK
2、 implementation of ISO 19012-2:2013.It supersedes BS ISO 19012-2:2009 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee CPW/172, Optics and Photonics.A list of organizations represented on this committee can beobtained on request to its secretary.This pub
3、lication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2013.Published by BSI Standards Limited 2013 ISBN 978 0 580 78875 8 ICS 37.020 Compliance with a British Standard cannot confer immunity
4、 fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 28 February 2013. Amendments issued since publicationDate T e x t a f f e c t e dBS ISO 19012-2:2013 ISO 2013Microscopes Designation of microscope objectives Part 2: Chroma
5、tic correctionMicroscopes Dsignation des objectifs de microscope Partie 2: Correction chromatiqueINTERNATIONAL STANDARDISO19012-2Second edition2013-02-01Reference numberISO 19012-2:2013(E)BS ISO 19012-2:2013ISO 19012-2:2013(E)ii ISO 2013 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2013All ri
6、ghts reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from
7、either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 19012-2:2013ISO 19012-2:2013(E) ISO 2013 All rights
8、 reserved iiiContents PageForeword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 24.1 Basic criterion for the depth of field. 24.2 Markings . 24.3 Specifications 2Annex A (informative) Depth of field, ob4Bibliography 5BS ISO 19012-2:2013ISO 19012-2:2013(E)ForewordISO
9、 (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical com
10、mittee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrot
11、echnical standardization.International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the membe
12、r bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying
13、 any or all such patent rights.ISO 19012-2 was prepared by Technical Committee ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes.This second edition cancels and replaces the first edition (ISO 19012-2:2009), 4.3.2 of which has been technically revised.ISO 19012 consists
14、 of the following parts, under the general title Microscopes Designation of microscope objectives: Part 1: Flatness of field/Plan Part 2: Chromatic correctionThe following parts are under preparation: Part 3: Spectral transmittanceiv ISO 2013 All rights reservedBS ISO 19012-2:2013INTERNATIONAL STAND
15、ARD ISO 19012-2:2013(E)Microscopes Designation of microscope objectives Part 2: Chromatic correction1 ScopeThis part of ISO 19012 specifies classes of chromatic correction and defines minimum requirements regarding chromatic correction. The defined marking on the component enables the operator to co
16、rrectly use the microscope.The standard application for visual observation refers to the combination of objective and tube lens as specified by the manufacturer. The specifications regarding chromatic correction only refer to axial chromatic aberration.2 Normative referencesThe following referenced
17、documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part
18、1: Light microscopy3 Terms and definitionsFor the purposes of this document, the terms and definitions given in ISO 10934-1 and the following apply.3.1reference wavelengthwavelength of 546,07 nm (e-line)3.2blue wavelengthwavelength of 479,99 nm (F-line)3.3red wavelengthwavelength of 643,85 nm (C-lin
19、e)3.4focusbest focusing point for each wavelength3.5focus differenceaxial separation of foci for different wavelengths ISO 2013 All rights reserved 1BS ISO 19012-2:2013ISO 19012-2:2013(E)4 Requirements4.1 Basic criterion for the depth of fieldFormula (1) applies as the basic criterion for the depth
20、of field:ob=nNA22(1)wheren is the refractive index of medium in object space;NA is the numerical aperture of objective; is the wavelength of the reference wave e-line in micrometers.A table of obdepending on NA can be found in Annex A.4.2 Markings4.2.1 GeneralThe following markings may be used if th
21、e requirements according to 4.3 are met.The indication of this marking does not apply to objective lenses sold before the year 2011.This part of ISO 19012 does not apply to the objectives exclusively used on stereomicroscopes.A mixture of a capital letter and a lowercase letter is allowed in marking
22、.4.2.2 AchromatMarking is not necessary but possible.ACH, ACHRO, ACHROMAT4.2.3 SemiapochromatObjective lenses shall be marked with one of the following three options:a) SEMIAPO, orb) FL, orc) a naming containing the letter sequence FLU.4.2.4 ApochromatAPO4.3 Specifications4.3.1 GeneralThe specificat
23、ions of the “Semiapochromat” and “Apochromat” include the criterion of “Achromat”.2 ISO 2013 All rights reservedBS ISO 19012-2:2013ISO 19012-2:2013(E)4.3.2 AchromatThe absolute value of the focus difference between the red wavelength and the blue wavelength is 2 ob.4.3.3 SemiapochromatThe absolute v
24、alues of the focus differences for the red wavelength and the blue wavelength to the reference wavelength are 2,5 ob.4.3.4 ApochromatThe absolute values of the focus differences for the red wavelength and the blue wavelength to the reference wavelength are ob. ISO 2013 All rights reserved 3BS ISO 19
25、012-2:2013ISO 19012-2:2013(E)Annex A (informative) Depth of field, obDry Immersionn 1 n 1,518 (m) 0,546 (m) 0,546NA ob(m) NA ob(m)0,04 170,63 0,40 2,590,07 55,71 0,70 0,850,10 27,30 0,90 0,510,13 16,15 1,00 0,410,15 12,13 1,25 0,270,16 10,66 1,30 0,250,20 6,83 1,35 0,230,22 5,64 1,40 0,210,25 4,370,
26、30 3,030,35 2,230,40 1,710,45 1,350,50 1,090,55 0,900,60 0,760,65 0,650,70 0,560,75 0,490,80 0,430,85 0,380,90 0,340,95 0,304 ISO 2013 All rights reservedBS ISO 19012-2:2013ISO 19012-2:2013(E)Bibliography1 ISO 8578, Microscopes Marking of objectives and eyepieces ISO 2013 All rights reserved 5BS ISO
27、 19012-2:2013ISO 19012-2:2013(E) ISO 2013 All rights reservedICS 37.020Price based on 5 pagesThis page deliberately left blankBSI is the independent national body responsible for preparing British Standards and other standards-related publications, information and services. It presents the UK view o
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