1、BSI Standards PublicationBS ISO 19012-3:2015Microscopes Designation ofmicroscope objectivesPart 3: Spectral transmittanceBS ISO 19012-3:2015 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 19012-3:2015. The UK participation in its preparation was entrusted to T
2、echnicalCommittee CPW/172, Optics and Photonics.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British St
3、andards Institution 2015.Published by BSI Standards Limited 2015ISBN 978 0 580 79528 2 ICS 37.020 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2015.Am
4、endments/corrigenda issued since publicationDate T e x t a f f e c t e d ISO 2015Microscopes Designation of microscope objectives Part 3: Spectral transmittanceMicroscopes Dsignation des objectifs de microscope Partie 3: Facteur de transmission spectraleINTERNATIONAL STANDARDISO 19012-3First edition
5、 2015-02-15Reference number ISO 19012-3:2015(E)BS ISO 19012-3:2015ISO 19012-3:2015(E)ii ISO 2015 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2015All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, e
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7、 + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 19012-3:2015ISO 19012-3:2015(E)Contents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 14.1 General . 14.2 Spectral transmittance tab
8、le or diagram 14.3 OSTD description 2Bibliography 4 ISO 2015 All rights reserved iiiBS ISO 19012-3:2015ISO 19012-3:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International S
9、tandards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, als
10、o take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. I
11、n particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the element
12、s of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (
13、see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence
14、 to the WTO principles in the Technical Barriers to Trade (TBT), see the following URL: Foreword Supplementary information .The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes.ISO 19012 consists of the following parts, under
15、the general title Microscopes Designation of microscope objectives: Part 1: Flatness of field/Plan Part 2: Chromatic correction Part 3: Spectral transmittanceiv ISO 2015 All rights reservedBS ISO 19012-3:2015ISO 19012-3:2015(E)IntroductionThe spectral transmittance of microscope objectives is an imp
16、ortant parameter that allows users to choose the appropriate product for a given application. Certain elements of glass material, cements, coatings, and optical design will have an effect on the spectral transmittance characteristics of microscope objectives. For example, one can choose to emphasize
17、 broad ranges of wavelengths or peak transmission in narrow bands or trade-off aberrations vs wavelength range. Therefore, the design of the lens set determines its spectral transmittance. A standard way to represent the spectral transmittance as a result of its design criteria was developed to addr
18、ess this important requirement. ISO 2015 All rights reserved vBS ISO 19012-3:2015ISO 19012-3:2015(E)BS ISO 19012-3:2015Microscopes Designation of microscope objectives Part 3: Spectral transmittance1 ScopeThis part of ISO 19012 specifies the relation of spectral characteristics between optical desig
19、n and the description to microscope users for the spectral transmittance of objectives, as the guideline.NOTE This part of ISO 19012 does not apply to objectives exclusively used on stereomicroscopes.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in th
20、is document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part 1: Light mi
21、croscopy3 Terms and definitionsFor the purposes of this document, the terms and definitions given in ISO 10934-1 and the following apply.3.1OSTDobjective spectral transmittance by designspectral transmittance calculated under the following conditions:a) on-axis light path;b) internal absorption of t
22、ransparent materials according to specifications by the materials manufacturer is included;c) reflectance of thin film coatings on optical surfaces according to their nominal value is included;d) internal absorption and surface reflectance of immersion media and specimen covering is neglectedNote 1
23、to entry: OSTD is usually expressed as a percentage.4 Requirements4.1 GeneralTransmittance data for microscope objectives given in catalogues, instruction manuals, websites, or other sources shall be labelled as OSTD.When OSTD is used in the designation of microscope objectives according to this par
24、t of ISO 19012, then it shall be made in either of two ways described in 4.2 and 4.3.4.2 Spectral transmittance table or diagramSee Figure 1 for an example of an appropriate transmittance diagram.INTERNATIONAL STANDARD ISO 19012-3:2015(E) ISO 2015 All rights reserved 1BS ISO 19012-3:2015ISO 19012-3:
25、2015(E) %100 nm806040200300 400 500 600 700 800 900 1 000Key wavelength transmittanceFigure 1 Example of spectral transmittance diagram4.3 OSTD descriptionThe OSTD description shall be in accordance with Table 1.Table 1 OSTD of objectivesSpectral transmittance DescriptionaExamplesOSTD 80 %1 2orHigh
26、OSTD in 1 2EXAMPLE 1 Designation of an objective with trans-mittance OSTD 80 % in the wavelength range 400 nm to 700 nm:400 - 700orHigh OSTD in 400 7003orHigh OSTD at 3EXAMPLE 2 Designation of an objective with trans-mittance OSTD 80 % at the wavelength 1 064 nm:1 064orHigh OSTD at 1 064a1, 2, 3are
27、indications of wavelengths, in nanometres, and shall be included in the description.2 ISO 2015 All rights reservedBS ISO 19012-3:2015ISO 19012-3:2015(E)Spectral transmittance DescriptionaExamplesOSTD 25 %1 2orNominal OSTD in 1 2EXAMPLE 3 Designation of an objective with trans-mittance OSTD 25 % in t
28、he wavelength range 340 nm to 1 100 nm:340 1 100orNominal OSTD in 340 1 1003orNominal OSTD at 3EXAMPLE 4 Designation of an objective with trans-mittance OSTD 25 % at the wavelength 340 nm:340orNominal OSTD at 340a1, 2, 3are indications of wavelengths, in nanometres, and shall be included in the desc
29、ription.Table 1 (continued) ISO 2015 All rights reserved 3BS ISO 19012-3:2015ISO 19012-3:2015(E)Bibliography1 ISO 20473:2007, Optics and photonics Spectral bands4 ISO 2015 All rights reservedBS ISO 19012-3:2015BS ISO 19012-3:2015ISO 19012-3:2015(E) ISO 2015 All rights reservedICS 37.020Price based o
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