1、BS ISO 20408:2017Traditional Chinese medicine Panax notoginseng seeds andseedlingsBSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS ISO 20408:2017 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 20408:2017. The UK participation
2、 in its preparation was entrusted to TechnicalCommittee CH/100/1, Traditional Chinese Medicine.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsibl
3、e for its correctapplication. The British Standards Institution 2017. Published by BSI Standards Limited 2017ISBN 978 0 580 91626 7ICS 65.020.20Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Poli
4、cy and Strategy Committee on 31 January 2017.Amendments/Corrigenda issued since publicationDate Text affectedBS ISO 20408:2017 ISO 2017Traditional Chinese medicine Panax notoginseng seeds and seedlingsMdecine traditionnelle chinoise Graines et plants de Panax notoginsengINTERNATIONAL STANDARDISO2040
5、8First edition2017-01Reference numberISO 20408:2017(E)BS ISO 20408:2017ISO 20408:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise i
6、n any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de B
7、landonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 20408:2017ISO 20408:2017(E)Foreword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Descriptions 34.1 Panax notoginseng seed . 34.2 Panax notoginseng see
8、dling . 45 Requirements 55.1 General characteristics . 55.2 Panax notoginseng seed . 55.2.1 Morphological features of seed . 55.2.2 Moisture 55.2.3 Purity . 65.2.4 Viability 65.2.5 Maturity . 65.2.6 Fungi . 65.2.7 1 000-seed weight, seed width, seed thickness and seed length . 65.3 Panax notoginseng
9、 seedling . 65.3.1 Morphological features of seedling 65.3.2 Fungi . 65.3.3 Nematode . 65.3.4 Seedling weight . 76 Sampling 76.1 Seed sampling 76.2 Seedling sampling 77 Test methods . 77.1 Moisture content . 77.2 Seed width 77.3 Seed thickness . 77.4 Seed length . 87.5 Maturity 87.6 Purity . 87.7 Se
10、ed viability. 87.8 1 000-seed weight 87.9 Fungi testing 87.9.1 Preparation of seeds . 87.9.2 Preparation of seedlings . 87.9.3 Alternaria spp. testing . 97.9.4 Fusarium spp. testing . 97.10 Nematode testing 97.11 Seedling weight 97.12 Seedling diameter . 97.13 Hibernaculum diameter 98 Test report .
11、99 Packaging, storage and transportation 1010 Marking .10Bibliography .11 ISO 2017 All rights reserved iiiContents PageBS ISO 20408:2017ISO 20408:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The wo
12、rk of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governm
13、ental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in th
14、e ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the po
15、ssibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list
16、 of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as wel
17、l as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.The committee responsible for this document is ISO/TC 249, Traditional Chinese medicine.iv ISO 2017 All rights res
18、ervedBS ISO 20408:2017INTERNATIONAL STANDARD ISO 20408:2017(E)Traditional Chinese medicine Panax notoginseng seeds and seedlings1 ScopeThis document specifies minimum requirements and test methods for seeds and seedlings of Panax notoginseng (Burk.) F. H. Chen. It is suitable for marketing of cultiv
19、ated Panax notoginseng seeds and seedlings. It is also suitable to be used for quality assurance by cultivators of Panax notoginseng.2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. Fo
20、r dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.International Seed Testing Association (ISTA): 2016, International Rules for Seed TestingInternational Seed Testing Association (ISTA): 2003, W
21、orking Sheets on Tetrazolium testing3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological databases for use in standardization at the following addresses: IEC Electropedia: available at h t t p :/ www .electropedia .or
22、g/ ISO Online browsing platform: available at h t t p :/ www .iso .org/ obp3.11 000-seed weightaverage weight of every 1 000 pure seeds of a test sample (3.19)3.2composite sampleportion formed by combining and mixing all the primary samples (3.8) taken from the lot (3.6)3.3foreign matterparts of Pan
23、ax notoginseng seeds (3.11) or seedlings (3.15) other than those named with the limits specified for the herbal materials concernedNote 1 to entry: Foreign matter is any organism, part or product of an organism, other than that named in the specification and description of Panax notoginseng seeds or
24、 seedlings.Note 2 to entry: Foreign matter is mineral admixtures not adhering to Panax notoginseng seeds or seedlings, such as soil, stones, sand and dust.3.4hibernaculumhibernated bud with bud scales ISO 2017 All rights reserved 1BS ISO 20408:2017ISO 20408:2017(E)3.5hibernaculum diameterlargest dis
25、tance at the site of the hibernaculum (3.4)Note 1 to entry: See Figure 2.Note 2 to entry: It is expressed in centimetres.3.6lotspecified quantity of seeds (3.11) or seedlings (3.15) that is physically and uniquely identifiable3.7maturitypercentage of mature seeds, determined by number, in the test s
26、ample (3.19)3.8primary sampleportion taken from the lot (3.6) during one single sampling action3.9purityweight of pure seed fraction over the total weight of the test sample (3.19)Note 1 to entry: The pure seed refers to the species stated by the applicant or found to predominate in the test and inc
27、ludes all botanical varieties and cultivars of that species.Note 2 to entry: It is expressed in per cent.3.10sealedclosed in such a way that the container cannot be opened to get access to the seed and be closed again without either destroying the seal or leaving evidence of tamperingNote 1 to entry
28、: This definition refers to the sealing of seed lots (3.6), as well as of seed samples.3.11seedmature ovules produced by Panax notoginseng (Burk.) F. H. Chen, consisting of three basic parts: embryo, endosperm and seed coat3.12seed lengthlargest distance from the lower part to the topNote 1 to entry
29、: See Figure 1.Note 2 to entry: It is expressed in millimetres.3.13seed thicknesslargest distance from the side perpendicular to the ridge to the opposite sideNote 1 to entry: See Figure 1.Note 2 to entry: It is expressed in millimetres.3.14seed widthlargest distance from the side of the raphe to th
30、e oppositeNote 1 to entry: See Figure 1.2 ISO 2017 All rights reservedBS ISO 20408:2017ISO 20408:2017(E)Note 2 to entry: It is expressed in millimetres.3.15seedlingyoung plant of Panax notoginseng (Burk.) F. H. Chen, which, after cultivation for one year, consists of hibernaculum (3.4), tap root (3.
31、18), lateral root and fibrous root3.16seedling diameterlargest distance at the site of the taprootNote 1 to entry: See Figure 2.Note 2 to entry: It is expressed in centimetres.3.17seedling weightaverage weight of test samples (3.19) of seedlings (3.15)3.18tap rootroot generated from the one year dev
32、elopment of the radicle after seed germination3.19test sampleportion of the composite sample (3.2) to which one of the test required in this standard is appliedNote 1 to entry: Test samples may be packed in different materials meeting conditions for specific tests e.g. moisture or purity (3.9).3.20v
33、iabilitypotential ability of a seed to germinate, or capability of an embryo to live, as a percentage of stained seeds in the test sample (3.19)Note 1 to entry: The percentage of stained seeds in the test sample shall be estimated by the method of Topographical Tetrazolium Test .4 Descriptions4.1 Pa
34、nax notoginseng seedIn this document, Panax notoginseng seed is the dehydrated seed of plant Panax notoginseng (Burk.) F. H. Chen consisting of three basic parts: embryo, endosperm and the seed coat, as shown in Figure 1. ISO 2017 All rights reserved 3BS ISO 20408:2017ISO 20408:2017(E)a) Faade of th
35、e seed b) Back of the seed123457689Key1 seed width2 seed length3 seed thickness4 circular water hole5 raphe6 seed coat7 endosperm8 embryo9 cavity of seedFigure 1 Structure of Panax notoginseng seed4.2 Panax notoginseng seedlingPanax notoginseng seedling is one-year-old seedling, consisting of four p
36、arts: hibernaculum, taproot, lateral root and fibrous root. For practical application, Panax notoginseng seedlings are classified into two groups on the basis of their morphology: long seedlings and short seedlings, as shown in Figure 2.4 ISO 2017 All rights reservedBS ISO 20408:2017ISO 20408:2017(E
37、)123456Key1 hibernaculum2 lateral root3 taproot4 fibrous root5 seedling diameter6 hibernaculum diameterFigure 2 Panax notoginseng seedling5 Requirements5.1 General characteristicsThe following requirements shall be met before separating the composite sample into test samples.a) Panax notoginseng see
38、ds shall be clean and free from foreign matter.b) Panax notoginseng seedlings shall be healthy and intact.c) The presence of mouldy seeds and external contaminants which are visible to the naked eye shall not be permitted.5.2 Panax notoginseng seed5.2.1 Morphological features of seedThe colour of th
39、e seed shall be white or yellowish white. The seed shall be hard-texture, without any unusual smell or mildew.5.2.2 MoistureThe moisture content in percentage mass shall not be less than 60 %. ISO 2017 All rights reserved 5BS ISO 20408:2017ISO 20408:2017(E)5.2.3 PuritySeed purity shall not be less t
40、han 98 %.5.2.4 ViabilityViability shall not be less than 90 %.5.2.5 MaturityMaturity shall not be less than 90 %.5.2.6 FungiNeither Fusarium spp. nor Alternaria spp. shall be detected.5.2.7 1 000-seed weight, seed width, seed thickness and seed lengthThe 1 000-seed weight, seed width, seed thickness
41、 and seed length shall comply with the requirements in Table 1.Table 1 Grading requirements of Panax notoginseng seedGrade1 000-seed weightagSeed width mmSeed thickness mmSeed length mmFirstb100 5,5 5,5 6,3Secondc80 to 100 5,0 to 5,5 5,0 to 5,5 5,5 to 6,3Thirdd60 to 80 4,5 to 5,0 4,5 to 5,0 5,0 to 5
42、,5Unqualified 60 4,5 4,5 5,0NOTE The above requirements were established on the basis of seeds collected from different regions.aThe 1 000-seed weight shall be determined when the moisture content of the seed is 60 % or slightly above.bThe seeds, of which the seed width is not less than 5,5 mm, shal
43、l not be less than 95 %. Otherwise, it shall be judged to be the second grade.cThe seeds, of which the seed width is not less than 5,0 mm, shall not be less than 95 %. Otherwise, it shall be judged to be the third grade.dThe seeds, of which the seed width is not less than 4,5 mm, shall not be less t
44、han 95 %. Otherwise, it shall be judged to be the unqualified grade.5.3 Panax notoginseng seedling5.3.1 Morphological features of seedlinga) The taproot and lateral root of seedling shall be intact, without diseases and insects. Taproot shall be solid.b) The hibernaculum of seedling shall be intact
45、and solid, without diseases and insects.5.3.2 FungiNeither Fusarium spp. nor Alternaria spp. shall be detected.5.3.3 NematodeNematode shall not be detected.6 ISO 2017 All rights reservedBS ISO 20408:2017ISO 20408:2017(E)5.3.4 Seedling weightThe seedling weight shall comply with the requirements in T
46、able 2.Table 2 Grading requirements of Panax notoginseng seedlingGradingSeedling weight gSeedling diameter cmHibernaculum diameter cmFirst 2,5 1,2 0,6Second 1,5 to 2,5 0,9 to 1,2 0,4 to 0,6Third 1,0 to 1,5 0,6 to 0,9 0,2 to 0,4Unqualified 1,0 0,6 0,26 Sampling6.1 Seed samplingThe seed sampling shall
47、 be carried out in accordance with ISTA, International Rules for Seed Testing: 2016, Chapter 2. The maximum weight of lot and minimum weight of sample are specified in Table 3.Table 3 Maximum weight of lot and minimum weight of sampleMaximum weight of seed lot (kg)Minimum weight of test sample (g)Fo
48、r measure of 1 000-seed weight, width, thickness and lengthFor purity analysis For other tests2 000 500 100 300NOTE The establishment of the above requirement is based on seeds collected from different regions and different treatment.6.2 Seedling samplingThe maximum seedling lot shall be 300 000 see
49、dlings and the minimum composite samples shall be 100 seedlings.7 Test methods7.1 Moisture contentDetermination of the mass fraction of moisture shall be in accordance with the high constant temperature method specified in ISTA, International Rules for Seed Testing.7.2 Seed widthTake 100 to 150 intact seeds in duplicate from the composite sample using the quartering method. The seed width shall be measured using a Vernier caliper in millimetres (to two decimal places). If the difference between measured values of the du
