1、BRITISH STANDARD BS ISO 21389:2004 Oil of gum turpentine, Chinese (mainly from Pinus massoniana Lamb.) ICS 71.100.60 BS ISO 21389:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 1 July 2004 BSI 1 July 2004 ISBN 0 580 44007 9 National for
2、eword This British Standard reproduces verbatim ISO 21389:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can
3、 be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of t
4、he BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid en
5、quirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This d
6、ocument comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. N
7、o. Date Comments Reference number ISO 21389:2004(E) OSI 4002INTERNATIONAL STANDARD ISO 21389 First edition 2004-06-15 Oil of gum turpentine, Chinese (mainly from Pinus massoniana Lamb.) Huile essentielle de trbenthine, type Chine (principalement de la gomme de Pinus massoniana Lamb.) BSISO21389:2004
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11、sleb nevwo. ISO 4002 All irthgs erse.devr lnUeto sswrehise specified, on trap fo this lbupictaion maeb y cudorperro de tuilizi den yna form ro na ybm ynae,s lecetrinoc ro mecinahcal, inclidung tohpcoiypodna gn micrfoilm, wittuoh repmissii non writign from ietI rehSa Ot tsserdda eh ebolw or ISOs memr
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13、evr iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for wh
14、ich a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all
15、matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are ci
16、rculated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held respo
17、nsible for identifying any or all such patent rights. ISO 21389 was prepared by Technical Committee ISO/TC 54, Essential oils. BSISO21389:2004blank 4002:98312OSISBINTENRATIONAL TSANDADR IS:98312 O4002(E)I SO 4002 All irthgs ersedevr 1Oil of gum turpentine, Chinese (mainly from Pinus massoniana Lamb.
18、) 1 Scope This International Standard specifies certain characteristics of the oil of gum turpentine, Chinese (mainly from Pinus massoniana Lamb.), in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of thi
19、s document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for la
20、belling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation
21、 of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 3405, Petroleum products Determination of distillation characteristics at atmospheric pressure ISO 4715, Essential oils Quantitative evaluation of residue on evaporation ISO 11024-1, Essential oils General guidance o
22、n chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of t
23、his document, the following terms and definitions apply. 3.1 oil of gum turpentine, Chinese essential oil obtained by steam distillation of the gum resin mainly from Pinus massoniana Lamb., of the Pinaceae family, growing in the south of China NOTE For information on the CAS number, see ISO/TR 21092
24、. 4 Requirements 4.1 Appearance Clear, transparent, mobile liquid. 4.2 Colour Colourless. 4.3 Odour Characteristic of gum turpentine. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,850 Maximum: 0,872 4.5 Refractive index at 20 C Minimum: 1,465 0 Maximum: 1,473 0 4.6 Optical rotation at 20 C Betwee
25、n 35 and 10. BSISO21389:2004IS:98312 O4002(E) 2 I SO 4002 All irthgs ersedevr4.7 Miscibility in ethanol, 90 % (volume fraction), at 20 C It shall not be necessary to use more than 7 volumes of ethanol, 90 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value
26、Maximum: 1,0 4.9 Distillation characteristics The temperature at the beginning of the distillation shall be higher than 150 C, and at least 90 % of the distillate shall be obtained at a temperature not exceeding 170 C. 4.10 Residue on evaporation Maximum: 2,5 % 4.11 Chromatographic profile Analysis
27、of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the c
28、hromato- graphic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene 65 90 Camphene traces 2,5 -Pinene 3,0 18 -3-Carene traces 0,3 Myrcene traces 1,5 Limonene traces 5,0 p-Cymene traces 2,5 Longifolene traces 2,5 -Caryophyllene traces 3,0 Caryophyllene
29、 oxide traces 0,4 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 4.12 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 150 ml. NOTE This volume allows each of the t
30、ests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 90 % (volume fraction), at 20 C See ISO 875
31、. 6.5 Acid value See ISO 1242. 6.6 Distillation characteristics See ISO 3405. 6.7 Residue on evaporation See ISO 4715. Test portion: 2,0 g Evaporation time: 3 h 6.8 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSI
32、SO21389:2004IS:98312 O4002(E) I SO 4002 All irthgs ersedevr 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of gum turpentine, Chinese (mainly from Pinus massoniana Lamb.) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 -Pinene Ca
33、mphene -Pinene Myrcene -3-Carene p-Cymene Limonene Longifolene -Caryophyllene Caryophyllene oxide Column: fused silica capillary; length 30 m; internal diameter 0,25 mm Stationary phase: poly(methyl phenyl vinyl siloxane) (SE-54 ) Film thickness: 0,25 m Oven temperature: isothermal at 50 C for 5 min
34、, then temperature programming from 50 C to 80 C at a rate of 2 C/min and isothermal at 80 C for 5 min, then temperature programming from 80 C to 120 C at a rate of 4 C/min and isothermal at 120 C for 5 min, then temperature programming from 120 C to 200 C at a rate of 8 C/min, and finally isotherma
35、l at 200 C for 5 min Injector temperature: 250 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1,0 ml/min Split ratio: 70/1 Figure A.1 Typical chromatogram taken on an apolar column BSISO21389:2004IS:98312 O4002(E) 4 I
36、 SO 4002 All irthgs ersedevrPeak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 -Pinene Camphene -Pinene -3-Carene Myrcene Limonene p-Cymene Longifolene -Caryophyllene Caryophyllene oxide Column: fused silica capillary; length 30 m; internal diameter 0,32 mm Stationary phase: poly(ethylene
37、 glycol) 20 000/2-nitroterephthalic acid (FFAP ) Film thickness: 0,25 m Oven temperature: isothermal at 50 C for 5 min, then temperature programming from 50 C to 80 C at a rate of 2 C/min and isothermal at 80 C for 5 min, then temperature programming from 80 C to 120 C at a rate of 4 C/min and isoth
38、ermal at 120 C for 5 min, then temperature programming from 120 C to 200 C at a rate of 8 C/min, and finally isothermal at 200 C for 5 min Injector temperature: 250 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1,0 m
39、l/min Split ratio: 70/1 Figure A.2 Typical chromatogram taken on a polar column BSISO21389:2004IS:98312 O4002(E) I SO 4002 All irthgs ersedevr 5Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety servic
40、es require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is wide
41、variation in the chemical composition of essential oils; the volume of the sample needed for certain requirements would be too costly for high- priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specif
42、ied type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see I
43、SO/TR 11018. B.2 Flashpoint of the essential oil of gum turpentine, Chinese The mean value is +38 C. NOTE Obtained with “closed cup” equipment. BSISO21389:2004IS:98312 O4002(E) 6 I SO 4002 All irthgs ersedevrBibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of fl
44、ashpoint 2 ISO/TR 21092:2004, Essential oils Characterization BSISO21389:2004blankBS ISO 21389:2004 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Eur
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