1、BSI Standards PublicationBS ISO 3064:2015Essential oil of petitgrain, Paraguayan type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.)BS ISO 3064:2015 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 3064:2015.The UK participatio
2、n in its preparation was entrusted to TechnicalCommittee AW/54, Essential oils.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correc
3、tapplication. The British Standards Institution 2015. Published by BSI StandardsLimited 2015ISBN 978 0 580 74319 1ICS 71.100.60Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy C
4、ommittee on 31 January 2015.Amendments issued since publicationDate Text affectedBS ISO 3064:2015 ISO 2015Essential oil of petitgrain, Paraguayan type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.)Huile essentielle de petitgrain, type Paraguay (Citrus aurantium L.
5、var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.)INTERNATIONAL STANDARDISO3064Third edition2015-01-15Reference numberISO 3064:2015(E)BS ISO 3064:2015ISO 3064:2015(E)ii ISO 2015 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2015All rights reserved. Unless otherwise specified, no part
6、 of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member bod
7、y in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 3064:2015ISO 3064:2015(E) ISO 2015 All rights reserved iiiContents PageForeword iv1 Scope . 12 Normat
8、ive references 13 Terms and definitions . 14 Specifications . 14.1 Appearance 14.2 Colour 24.3 Odour . 24.4 Relative density, d2020. 24.5 Refractive index at 20 C . 24.6 Optical rotation at 20 C 24.7 Miscibility in ethanol 70 % (volume fraction) at 20 C 24.8 Acid value. 24.9 Chromatographic profile
9、24.10 Flashpoint 35 Sampling 36 Test methods . 36.1 Relative density, d2020. 36.2 Refractive index at 20 C . 36.3 Optical rotation at 20 C 36.4 Miscibility in ethanol 70 % (volume fraction) at 20 C 36.5 Acid value. 36.6 Chromatographic profile 37 Packaging, labelling, marking, and storage 4Annex A (
10、informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of petitgrain, Paraguay type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium L. var. bigaradia Hook f.) 5Annex B (informative) Flashpoint . 7Bibliography 8BS ISO 3064:2015ISO 3064:2015(E)ForewordI
11、SO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical c
12、ommittee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electr
13、otechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was dra
14、fted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent ri
15、ghts. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not consti
16、tute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThe comm
17、ittee responsible for this document is ISO/TC 54, Essential oils.This third edition cancels and replaces the second edition (ISO 3064:2000), which has been technically revised.iv ISO 2015 All rights reservedBS ISO 3064:2015INTERNATIONAL STANDARD ISO 3064:2015(E)Essential oil of petitgrain, Paraguaya
18、n type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.)1 ScopeThis International Standard specifies certain characteristics of the essential oil of petitgrain, Paraguayan type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.), intended
19、 to facilitate the assessment of its quality.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of
20、the referenced document (including any amendments) applies.ISO/TS 210, Essential oils General rules for packaging, conditioning and storageISO/TS 211, Essential oils General rules for labelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative
21、 density at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of miscibility in ethanolISO 1242, Essential oils Determination of acid valueISO 11024 (all parts), Essential oils Gen
22、eral guidance on chromatographic profiles3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1essential oil of petitgrainessential oil obtained by steam distillation of only leaves and twigs of Citrus aurantium L. var. Paraguay (syn. Citrus aurantium
23、 var. bigaradia Hook f.), mainly cultivated in Paraguay, of the Rutaceae familyNote 1 to entry: For information on the CAS number, see ISO/TR 21092.24 Specifications4.1 AppearanceClear liquid. ISO 2015 All rights reserved 1BS ISO 3064:2015ISO 3064:2015(E)4.2 ColourColourless to pale yellow.4.3 Odour
24、Fresh, green, and flowery.4.4 Relative density, d2020Minimum: 0,884.Maximum: 0,892.4.5 Refractive index at 20 CMinimum: 1,454.Maximum: 1,463.4.6 Optical rotation at 20 CBetween 5 and 1.4.7 Miscibility in ethanol 70 % (volume fraction) at 20 CIt shall not be necessary to use more than 3,5 volumes of
25、ethanol 70 % (volume fraction) to obtain a clear solution with one volume of essential oil.4.8 Acid valueMaximum: 1.4.9 Chromatographic profileCarry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representative and characteristic components sh
26、own in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil.Table 1 Chromatographic profileComponent Minimum%Maximum%-Pinene 0,5 2,0Myrcene 1,3 3,0Sabinene 0,1 0,5Limonene 0,7 3,5(Z)
27、-Ocimene 0,7 1,5(E)-Ocimene 0,5 3,5Linalool 15,0 30,0Linalyl acetate 40,0 60,0NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A.2 ISO 2015 All rights reservedBS ISO 3064:2015ISO 3064:2015(E)Component Minimum%Maximum%-Caryophyllene 0,3 1
28、,5-Terpineol 3,0 7,0Nerol 0,5 2,0Geraniol 2,0 4,5Neryl acetate 1,0 3,0Geranyl acetate 2,0 5,0NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A.4.10 FlashpointInformation concerning the flashpoint is given in Annex B.5 SamplingSampling s
29、hall be performed in accordance with ISO 212.Minimum volume of the test sample: 25 ml.NOTE This volume makes it possible for each of the tests specified in this International Standard to be carried out at least once.6 Test methods6.1 Relative density, d2020Determine the relative density in accordanc
30、e with ISO 279.6.2 Refractive index at 20 CDetermine the refractive index in accordance with ISO 280.6.3 Optical rotation at 20 CDetermine the optical rotation in accordance with ISO 592.6.4 Miscibility in ethanol 70 % (volume fraction) at 20 CDetermine the miscibility in accordance with ISO 875.6.5
31、 Acid valueDetermine the acid value in accordance with ISO 1242.6.6 Chromatographic profileDetermine the chromatographic profile in accordance with ISO 11024.Table 1 (continued) ISO 2015 All rights reserved 3BS ISO 3064:2015ISO 3064:2015(E)7 Packaging, labelling, marking, and storageThese items shal
32、l be in accordance with ISO/TS 210 and ISO/TS 211.4 ISO 2015 All rights reservedBS ISO 3064:2015ISO 3064:2015(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of petitgrain, Paraguay type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantiu
33、m L. var. bigaradia Hook f.)Peak identification Operating conditions: Column: dimethylpolysiloxane (HP-1 or BP-1a), length 30 m, internal diameter 0,25 mmFilm thickness: 0,25 mOven temperature: temperature programmed 1 min at 60 C, then from 60 C to 240 C at a rate of 2 C/minInjector temperature: 25
34、0 CDetector temperature: 300 CDetector: flame ionization typeCarrier gas: nitrogenVolume injected: 0,5 lCarrier gas flow rate: 1 ml/minSplit: 1/1001 -Pinene2 Sabinene3 Myrcene4 Limonene5 (Z)-Ocimene6 (E)-Ocimene7 Terpinolene8 Linalool9 Linalyl acetate10 -Caryophyllene11 -Terpineol12 Geranyl acetate1
35、3 Neryl acetate14 Nerol15 Geraniol16 Terpenyl acetateaHP-1 and BP-1 are examples of suitable products available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of these products.Figure A.1 Typical chromatogram of ess
36、ential oil of petitgrain taken on an apolar column ISO 2015 All rights reserved 5BS ISO 3064:2015ISO 3064:2015(E)Peak identification Operating conditions: Column: polyethylene glycol (HP innowax or BP 20a), length 30 m, internal diameter 0,25 mmFilm thickness: 0,25 mOven temperature: temperature pro
37、grammed 1 min at 60 C, then from 60 C to 240 C at a rate of 2 C/minInjector temperature: 250 CDetector temperature: 300 CDetector: flame ionization typeCarrier gas: nitrogenVolume injected: 0,5 lCarrier gas flow rate: 1 ml/minSplit: 1/1001 -Pinene2 Sabinene3 Myrcene4 Limonene5 (Z)-Ocimene6 (E)-Ocime
38、ne7 Terpinolene8 Linalool9 Linalyl acetate10 -Caryophyllene11 -Terpineol12 Geranyl acetate13 Neryl acetate14 Nerol15 Geraniol16 Terpenyl acetateaHP innowax or BP 20 are examples of suitable products available commercially. This information is given for the convenience of users of this document and d
39、oes not constitute an endorsement by ISO of these products.Figure A.2 Typical chromatogram of essential oil of petitgrain taken on a polar column6 ISO 2015 All rights reservedBS ISO 3064:2015ISO 3064:2015(E)Annex B (informative) FlashpointB.1 General informationFor safety reasons, transport companie
40、s, insurance companies, and people in charge of safety services require information on the flash points of essential oils, which in most cases are flammable products.A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was difficult to recommend a single appa
41、ratus for standardization purposes, given the following: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high priced essential oils; as there are several different types of equipment which can be
42、 used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard for information, in order to meet the requirements of the interested parties.The equipment with which this
43、value was obtained has to be specified.For further information, see ISO/TR 11018.1B.2 Flashpoint of the essential oil of petitgrain, Paraguayan typeThe mean value is +75 C.NOTE Obtained with Luchaire1)equipment.1) Luchaire is an example of a suitable equipment available commercially. This informatio
44、n is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product. ISO 2015 All rights reserved 7BS ISO 3064:2015ISO 3064:2015(E)Bibliography1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint2 ISO/TR 21092, Essentia
45、l oils Characterization8 ISO 2015 All rights reservedBS ISO 3064:2015BS ISO 3064:2015ISO 3064:2015(E) ISO 2015 All rights reservedICS 71.100.60Price based on 8 pagesThis page deliberately left blankBSI is the national body responsible for preparing British Standards and other standards-related publi
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