1、 g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58ICS 71.100.60Oil of sage, Spanish (Salvia lavandulifoliaVahl)BRITISH STANDARDBS ISO 3526:2005BS ISO
2、 3526:2005This British Standard was published under the authority of the Standards Policy and Strategy Committee on 17 January 2006 BSI 17 January 2006ISBN 0 580 46880 1The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue und
3、er the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct appl
4、ication. Compliance with a British Standard does not of itself confer immunity from legal obligations.Summary of pagesThis document comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover.The BSI copyrigh
5、t notice displayed in this document indicates when the document was last issued.Amendments issued since publicationAmd. No. Date CommentsA list of organizations represented on this committee can be obtained on request to its secretary.Cross-referencesenquiries on the interpretation, or proposals for
6、 change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK.National forewordThis British Standard reproduces verbatim ISO 3526:2005 and implements it as the UK national standard.The UK participation in its preparation was entrusted
7、to Technical Committee AW/54, Essential oils, which has the responsibility to: aid enquirers to understand the text; present to the responsible international/European committee any Reference numberISO 3526:2005(E)INTERNATIONAL STANDARD ISO3526Third edition2005-07-01Oil of sage, Spanish (Salvia lavan
8、dulifolia Vahl) Huile essentielle de sauge dEspagne (Salvia lavandulifolia Vahl) BS ISO 3526:2005ii iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normal
9、ly carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the
10、 work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare I
11、nternational Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some o
12、f the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 3526 was prepared by Technical Committee ISO/TC 54, Essential oils. This third edition cancels and replaces the second edition (ISO 3526:1991), wh
13、ich has been technically revised. BS ISO 3526:2005blank1Oil of sage, Spanish (Salvia lavandulifolia Vahl) 1 Scope This International Standard specifies certain characteristics of the oil of sage, Spanish (Salvia lavandulifolia Vahl), in order to facilitate assessment of its quality. 2 Normative refe
14、rences The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules f
15、or packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 59
16、2, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentat
17、ion in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of sage, Spanish essent
18、ial oil obtained by steam distillation of the aerial part of the flowering plant of Salvia lavandulifolia Vahl, of the Lamiaceae family, growing wild or cultivated in Spain or in any part of the world NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Liquid. 4.2
19、 Colour From colourless to pale yellow. 4.3 Odour Characteristic, camphoraceous, herbaceous. 4.4 Relative density at 20 C, 2020dMinimum: 0,907 Maximum: 0,932 4.5 Refractive index at 20 C Minimum: 1,465 0 Maximum: 1,473 0 4.6 Optical rotation at 20 C Between +7 and +17. BS ISO 3526:20052 4.7 Miscibil
20、ity in ethanol, 80 % (volume fraction), at 20 C It shall not be necessary to use more than 2 volumes of ethanol, 80 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 1,0 4.9 Chromatographic profile Analysis of the essential oil shall be carried o
21、ut by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromato-graphic profile of the essentia
22、l oil. Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene 4 11 Sabinene 0,1 3,5 Limonene 2 6 1,8-Cineole 10 30 Linalol 0,3 4 Camphor 11 36 Borneol 1 7 Terpinen-4-ol 2 Linalyl acetate 0,1 5 -Terpinyl acetate 0,5 9 Sabinyl acetate 0,5 9 NOTE The chromatographic profile is normative,
23、 contrary to typical chromatograms given for information in Annex A. 4.10 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out a
24、t least once. 6 Test methods 6.1 Relative density at 20 C, 2020dSee ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 80 % (volume fraction), at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Chromatographic profile See ISO 1
25、1024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 3526:20053Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of sage, Spanish (Salvia lavandulifolia Vahl) Peak identification 1 -Pinene 8 Bor
26、neol 2 Camphene 9 Terpinen-4-ol 3 Sabinene 10 Linalyl acetate 4 -Pinene 11 Bornyl acetate 5 Limonene + 1,8-cineole 12 Sabinyl acetate 6 Linalol 13 -Terpinyl acetate 7 Camphor 14 -Caryophyllene Operating conditions Column: capillary; length 60 m; internal diameter 0,25 mm Stationary phase: poly(dimet
27、hyl siloxane) (DB-1) Film thickness: 0,25 m Oven temperature: temperature programming from 80 C to 110 C at a rate of 3 C/min, then isothermal at 110 C for 10 min, then temperature programming from 110 C to 140 C at a rate of 4 C/min, from 140 C to 250 C at a rate of 6 C/min and isothermal at 250 C
28、for 5 min Injector temperature: 260 C Detector temperature: 270 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,2 l Carrier gas flow rate: 1 ml/min Split ratio: 1/60 Figure A.1 Typical chromatogram taken on an apolar column BS ISO 3526:20054 Peak identification 1 -Pinene 9 L
29、inalyl acetate 2 Camphene 10 Bornyl acetate 3 -Pinene 11 Terpinen-4-ol 4 Sabinene 12 -Caryophyllene 5 Limonene 13 Sabinyl acetate 6 1,8-Cineole 14 -Terpinyl acetate 7 Camphor 15 Borneol 8 Linalol Operating conditions Column: capillary; length 60 m; internal diameter 0,25 mm Stationary phase: poly(et
30、hylene glycol) TPA modified (SP-1 000) Film thickness: 0,25 m Oven temperature: temperature programming from 95 C to 190 C at a rate of 4 C/min, then isothermal at 190 C for 8 min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: nitrogen Volume inj
31、ected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 3526:20055Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services requ
32、ire information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study of the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is wide variati
33、on in the chemical composition of essential oils; the volume of the sample needed for certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type
34、 only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11
35、018. B.2 Flashpoint of the essential oil of sage, Spanish The mean value is + 49 C. NOTE Obtained with “Setaflash” equipment. BS ISO 3526:20056 Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092:2004, Essential oils Characterization BS
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