1、BRITISH STANDARD BS ISO 4718:2004 Oil of lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson ICS 71.100.60 BS ISO 4718:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 2 June 2004 BSI 2 June 2004 ISBN 0 580 43841 4 National fore
2、word This British Standard reproduces verbatim ISO 4718:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can b
3、e obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the
4、 BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enqu
5、irers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This doc
6、ument comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No.
7、 Date Comments Reference number ISO 4718:2004(E)INTERNATIONAL STANDARD ISO 4718 Second edition 2004-05-15 Oil of lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson Huile essentielle de lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson BSISO4718:2004DPlcsid Fremia ihTs PDF file
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10、aeino marapterew stpo ereimizde fro irptni.gn Evyre cera neeb sah takne tsne oeru taht teh file is siutlbae fosu re yb ISO memdob rebeis. In tlnu ehikletneve y ttah lborp aem lertait gno it is f,dnuo plsaee ifnrom ttneC ehlar Secterirata ta teh serddaig sleb nevwo. ii BSISO4718:2004 iiiForeword ISO
11、(the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical comm
12、ittee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrote
13、chnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the memb
14、er bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifyi
15、ng any or all such patent rights. ISO 4718 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 4718:1981), which has been technically revised. BSISO4718:2004blank BSISO4718:2004INTENRATIONAL TSANDADR IS:8174 O4002(E)1Oil of l
16、emongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson 1 Scope This International Standard specifies certain charac- teristics of the oil of lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson, in order to facilitate assessment of its quality. 2 Normative references The following ref
17、erenced documents are indispen- sable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditio
18、ning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Dete
19、rmination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatogr
20、aphic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of lemongrass essential oil obtained by steam distillation of the fresh aerial parts of Cymbopogon
21、 flexuosus (Nees ex Steudel) J.F. Watson, of the Poaceae family. NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear, mobile liquid. 4.2 Colour Pale yellow to yellowish brown. 4.3 Odour Strong, green, resembling that of citral. 4.4 Relative density at 20 C,
22、20 20 d Minimum: 0,885 Maximum: 0,905 4.5 Refractive index at 20 C Minimum: 1,483 Maximum: 1,489 4.6 Optical rotation at 20 C Between 4 and +1. BSISO4718:20042 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C It shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fr
23、action), to obtain a clear solution with 1 volume of essential oil. 4.8 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristics components shown in Table 1 shall be identified. The proporti
24、ons of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % Limonene 0,5 3,5 6-Methyl-5-heptene-2-one 0,1 2,0 Caryophyllene 0,2 3,5 Neral 25,0 35,0
25、 Geranial 35,0 47,0 Geranyl acetate 0,5 6,0 Geraniol 1,5 8,0 NOTE The chromatographic profile is normative, contrary to typical chromatogram given for information in Annex A. 4.9 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 m
26、l. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 70
27、% (volume fraction), at 20 C See ISO 875. 6.5 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSISO4718:2004 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of
28、lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson Peak identification Operating conditions 1 6-Methyl-5-hepten-2-one 2 Limonene 3 Neral 4 Geraniol 5 Geranial 6 Geranyl acetate 7 Caryophyllene Column: capillary; length 30 m; internal diameter 320 m Stationary phase: poly(dimethyl siloxane
29、) Film thickness: 0,25 m Oven temperature: isothermal at 70 C for 10 min, then temperature programming from 70 C to 220 C at a rate of 2 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0
30、,3 ml/min Figure A.1 Typical chromatogram taken on an apolar column BSISO4718:20044 Peak identification Operating conditions 1 Limonene 2 6-Methyl-5-hepten-2-one 3 Citronellal 4 Caryophyllene 5 Neral 6 Geranial 7 Geranyl acetate 8 Geraniol Column: capillary; length 10 m; internal diameter 0,100 mm S
31、tationary phase: poly(ethylene glycol) (Supercowax-10) Film thickness: 0,1 m Oven temperature: 60 C to 240 C at a rate of 12 C/min, then 240 C for 18 min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 1 l Carrier gas flo
32、w rate: 0,7 ml/min Split ratio: 150/1 Figure A.2 Typical chromatogram taken on a polar column BSISO4718:2004 5Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flas
33、hpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical com- p
34、osition of essential oils; the volume of the sample needed in certain requirements would be too costly for high- priced essential oils; as there are several different types of equip- ment which can be used for the determination, users cannot be expected to use one specified type only. Consequently,
35、it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint
36、of the essential oil of lemongrass The mean value is +89 C. NOTE Obtained with Pensky Martens equipment. BSISO4718:20046 Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092: 1) , Essential oils Characterization 1) To be published. BSISO
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