1、BRITISH STANDARD BS ISO 4733:2004 Oil of cardamom Elettaria cardamomum (L.) Maton ICS 71.100.60 BS ISO 4733:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 13 July 2004 BSI 13 July 2004 ISBN 0 580 44042 7 National foreword This British S
2、tandard reproduces verbatim ISO 4733:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on reque
3、st to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI El
4、ectronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers t
5、o understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document c
6、omprises a front cover, an inside front cover, the ISO title page, pages ii to iv, pages 1 to 7 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments Reference number ISO 4733:2004
7、(E) OSI 4002INTERNATIONAL STANDARD ISO 4733 Second edition 2004-07-01 Oil of cardamom Elettaria cardamomum (L.) Maton Huile essentielle de cardamome Elettaria cardamomum (L.) Maton BSISO4733:2004ISO 4733:2004(E) DPlcsid Fremia ihTs PDF file may ctnoian emdebt dedyfepcaes. In ccaocnadrw eith Aebods l
8、icensilop gnic,y this file mairp eb ynted iv roweb detu slahl ton ide ebtlnu deess the typefaces whice era hml era deddebicsnede to i dnanstlaled t noeh computfrep reormign tide ehtin.g In wodlnidaot gnhis file, trapise atpecc tiereht nser ehnopsiiblity fo not infriigngn Aebods licensilop gnic.y ehT
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10、b sah taken to sneeru that the file is suitlbae fosu re yb ISO memdob rebeis. In tlnu ehikletneve y ttah lborp aem leratit gno it is f,dnuo plsaee inform ttneC ehlar Secrteiraat ta the serddaig sleb nevwo. ISO 2004 All irthgs erse.devr lnUeto sswrehise specified, on trap fo this lbupictaion maeb y c
11、udorperro de tuilizi den yna form ro na ybm ynae,s lecetrinoc ro mecinahcal, inclidung tohpcoiypodna gn micrfoilm, wittuoh repmissii non writign from ietI rehSa Ot tsserdda eh ebolw or ISOs memreb i ydobn the cnuotrfo y ttseuqer ehe.r ISO cirypothg fofice saCe tsopale 65 eneG 1121-HC 02 av leT. 4 +
12、10 947 22 1 11 xaF0 947 22 14 + 9 74 E-mail coirypthgiso.o gr We bwww.is.o gro Pulbisdehi n Switlrez dnaii ISO 4002 Allr ithgsr esedevrBSISO4733:2004IS:3374 O4002(E) I SO 4002 All irthgs ersedevr iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of nation
13、al standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Internatio
14、nal organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rule
15、s given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at l
16、east 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 4733 was prepared by Technical Committee ISO/TC 54
17、, Essential oils. This second edition cancels and replaces the first edition (ISO 4733:1981), which has been technically revised. BSISO4733:2004INTERNATIONAL STANDARD ISO 4733:2004(E) ISO 2004 All rights reserved 1o liOf racmado melEc airattmumomadra (L). Maton epocS 1 Thsi retnItannoial atSnrads dc
18、epfisei certnia crahcaretistcis fo eht iol fo cramadom llEeratia craomadmum M ).L(anot., io nrder ot afciiltaet assessmfo tne sti auqlity. iv vitamroN 2e secnerefer Tf ehniwollor gferecnecod demustne rae dniissnepalbf ero pa ehtlpicnoita fo siht codmu.tne Fro r detadferecneseo ,lny de ehttic noiia d
19、etppseil. Fro tadnur deferecnesetal eht ,set deitifo no eht rferecnecod demu( tneicnlidua gnny mamdnestne) ppaseil. OSI/TR 012, sEsitnela oisl G reneal rusel rof capkigac ,gnoidnoitina gnns dtroega OSI/TR ,112 sEsitneo lails G rener lausel rof balelnila gm dnarkgni c fooiatnrens OSI 12,2 sEsitneaio
20、lsl maS plign OSI 72,9 sEsitneal iosl Deretmanitoifo n realvite snedyti 2 ta0 C eR refenchtem edo OSI 82,0 sEstneaiio lsl reteD mitannoi fo rrfecaviti exedn OSI 53,6 sEsitneal iosl rP eraptanoi t foest asmpels OSI 95,2 sEstneaiio lsl reteDmitannoi tpo focila itatorno OSI 07,9 sEsitneal iosl reteDman
21、itoifo n seret avlue OSI 78,5 sEsitneao lsli vEulaaito nof misciibytil ni ahtelon OSI 21,24 sEsitneao lsli Deretmanitoica fo nid vaeul OSI 11-420,1 sEstneaio lsli G reneal ugdicnao en crhotamorghpaci rpifolse raP t 1: rPperatafo noi chrootamrghpaci rpfoseli rof rpsetatneoini n stradnasd OSI 11-420,2
22、 sEstneaio lsli G reneal ugdicnao en crhotamorghpaci rpifolse raP t 2: itUliztao noif crhotamorghpaci rpfolise fo slpmase fo sesitnela iosl 3 d dna smreTesnoitinif Fro eht rupsopse fo tsih codmueht ,tne flloowign retms fed dnanitisnoi apply. 1.3 drac fo lioamom sesaitnel ioo lbatenid by stmae sidtli
23、talino fo hte frstiu fo craadmom ellEratic aardomammu L(.) otaMn fo eht Zignrebicaeaf emaily, rgowign mlniay C nirtneamA lreciG/ataumeala dna dnIi/arSi knaLa NOTE i roFnmrofitano t noC ehAmun S,reb ese OSI/TR .29012 stnemeriuqeR 4 .41 Apecnaraep qiLudi. .42 loCruo Almots oclourelt sso pale yelwol. .
24、43 Oruod rahCcaretistcis ,pciy nac dioencil. 44. Realvite densyti at 20 C, 02 02 d neCtA larmire/ac Gutaalame aidnI/ L irSnaak niMimum: 29,00 19,09 xaMimum: 49,039,0 06 BSISO4733:2004INTENRATIONAL TSANDADR IS:3374 O4002(E)I SO 4002 All irthgs ersedevr 1Oil of cardamom Elettaria cardamomum (L.) Maton
25、 1 Scope This International Standard specifies certain characteristics of the oil of cardamom Elletaria cardamomum (L.) Maton., in order to facilitate assessment of its quality. iv 2 Normative references The following referenced documents are indispensable for the application of this document. For d
26、ated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marki
27、ng of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 356, Essential oils Preparation of test samples ISO 592, Essential oils Determination of optical rotation
28、 ISO 709, Essential oils Determination of ester value ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for present
29、ation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of cardamom essential
30、 oil obtained by steam distillation of the fruits of cardamom Elletaria cardamomum (L.) Maton of the Zingiberaceae family, growing mainly in Central America/Guatemala and India/Sri Lanka NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Liquid. 4.2 Colour Almost
31、 colourless to pale yellow. 4.3 Odour Characteristic, spicy and cineolic. 4.4 Relative density at 20 C, 20 20 d Central America/ Guatemala India/ Sri Lanka Minimum: 0,920 0,919 Maximum: 0,940 0,936 BSISO4733:2004IS:3374 O4002(E) 2 I SO 4002 All irthgs ersedevr4.5 Refractive index at 20 C Central Ame
32、rica/ Guatemala India/ Sri Lanka Minimum: 1,460 1,460 Maximum: 1,467 1,468 4.6 Optical rotation at 20 C Central America/ Guatemala India/ Sri Lanka Between +24 and +39 Between +22 and +41 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C It shall not be necessary to use more than 3 volumes
33、 of ethanol, 70 % (volume fraction), to obtain a clear solution (sometimes opalescent) with 1 volume of essential oil. 4.8 Acid value Central America/ Guatemala India/ Sri Lanka Maximum: 6 6 4.9 Ester value Central America/ Guatemala India/ Sri Lanka Minimum: 92 92 Maximum: 150 150 4.10 Chromatograp
34、hic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. T
35、his constitutes the chromatographic profile of the essential oil. Table 1 Chromatographic profile Central America/ Guatemala India/ Sri Lanka Component Min. % Max. % Min. % Max. % -Pinene 1 2 1 2 Sabinene 3 5 2 4 Myrcene traces2,5 traces 2,5 Limonene 2 3 3 7 1,8-Cineole 27 35 23 33 Linalol 3 6 3,5 7
36、 Linalyl acetate 4 6 4 9 Terpinen-4-ol 0,8 1,5 1 3 -Terpineol traces 2,5 3 7 Terpinyl acetate 35 45 32 42 trans-Nerolidol 0,5 1 1 2 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 4.11 Flashpoint Information on the flashpoint is give
37、n in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Preparation of test sample See ISO 356. 7 Test methods 7.1 Relative density at 20 C, 20 20 d See ISO 279.
38、BSISO4733:2004IS:3374 O4002(E) I SO 4002 All irthgs ersedevr 37.2 Refractive index at 20 C See ISO 280. 7.3 Optical rotation at 20 C See ISO 592. 7.4 Miscibility in 70 % (volume fraction) ethanol at 20 C See ISO 875. 7.5 Acid value See ISO 1242. 7.6 Ester value See ISO 709. Test sample: 1 g. Saponif
39、ication time: 3 h. 7.7 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 8 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSISO4733:2004IS:3374 O4002(E) 4 I SO 4002 All irthgs ersedevrAnnex A (informative) Typical chromatograms of the analysis by gas chromatography o
40、f the essential oil of cardamom Elettaria cardamomum (L.) Maton Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 -Pinene Sabinene Myrcene Limonene 1,8-Cineole Linalol Terpinen-4-ol -Terpineol Linalyl acetate Terpinyl acetate trans-Nerolidol Column: fused silica capillary; length 25 m
41、; internal diameter 0,25 mm Stationary phase: poly(dimethyl siloxane) Film thickness: 0,25 m Oven temperature: temperature programming from 50 C to 230 C at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 230 C Detector: flame ionization type Carrier gas: helium Injection volume:
42、 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/90 Figure A.1 Typical chromatogram taken on an apolar column BSISO4733:2004IS:3374 O4002(E) I SO 4002 All irthgs ersedevr 5Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 -Pinene Sabinene Myrcene Limonene 1,8-Cineole Linalol Terp
43、inen-4-ol -Terpineol Linalyl acetate Terpinyl acetate trans-Nerolidol Column: fused silica capillary; length 25 m; internal diameter 0,22 mm Stationary phase: poly(ethylene glycol) (CARBOWAX 20 M) Film thickness: 0,25 m Oven temperature: temperature programming from 50 C to 230 C at a rate of 2 C/mi
44、n Injector temperature: 230 C Detector temperature: 230 C Detector: flame ionization type Carrier gas: helium Injection volume: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/90 Figure A.2 Typical chromatogram taken on a polar column BSISO4733:2004IS:3374 O4002(E) 6 I SO 4002 All irthgs ersede
45、vrAnnex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant me
46、thods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that there is wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high- p
47、riced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard in ord
48、er to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essential oil of cardamom The mean value is +59 C. NOTE Obtained with “Grabner” equipment. BSISO4733:2004IS:33
49、74 O4002(E) I SO 4002 All irthgs ersedevr 7Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092:2004, Essential oils Characterization BSISO4733:2004BS ISO 4733:2004 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent natio
