1、 DEUTSCHE NORM September 2006DIN EN 1390 ICS 71.100.50 Supersedes DIN V ENV 1390:1995-05 Wood preservatives Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae Laboratory method English version of DIN EN 1390:2006-09 Holzschutzmittel Bestimmung der bekmpfenden Wirkung
2、gegenber Larven von Hylotrupes bajulus (Linnaeus) Laboratoriumsverfahren Englische Fassung DIN EN 1390:2006-09 Document comprises 22 pages No part of this standard may be reproduced without prior permission of DIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,
3、 has the exclusive right of sale for German Standards (DIN-Normen). English price group 12 www.din.de www.beuth.de !,pr“01.07 9777927DIN EN 1390:2006-09 2 National foreword This standard has been prepared by CEN/TC 38 “Durability of wood and derived materials” (Secretariat: France). The responsible
4、German body involved in its preparation was the Normenausschuss Materialprfung (Materials Testing Standards Committee), Technical Committee NMP 412 Prfung von Holzschutzmitteln. Amendments This standard differs from DIN V ENV 1390:1995-05 as follows: a) Harmonized specifications have been introduced
5、 for test specimens used in diverse biological tests. b) The method has been split into a method for testing fast and slow acting preservatives over a test period of 12 weeks or 24 weeks and a method for testing deferred acting preservatives respectively over a test period of 52 weeks. c) The terms
6、given in EN 1001-1 and the definitions given in EN 1001-2 have been taken into account. d) An informative Annex specifying environmental, health and safety precautions within chemical/biological laboratories has been included. Previous editions DIN V ENV 1390:1995-05 EUROPEAN STANDARD NORME EUROPENN
7、E EUROPISCHE NORM EN 1390 June 2006 ICS 71.100.50 Supersedes ENV 1390:1994 English Version Wood preservatives - Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae - Laboratory method Produits de prservation du bois - Dtermination de laction curative contre les larves
8、dHylotrupes bajulus (Linnaeus) - Mthode de laboratoire Holzschutzmittel - Bestimmung der bekmpfeden Wirkung gegenber Larven von Hylotrupes bajulus (Linnaeus) - Laboratoriumsverfahren This European Standard was approved by CEN on 24 May 2006. CEN members are bound to comply with the CEN/CENELEC Inter
9、nal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member
10、. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members a
11、re the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and
12、 United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2006 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 1390:
13、2006: EEN 1390:2006 (E) 2 Contents Page Foreword3 Introduction .4 1 Scope 5 2 Normative references 5 3 Terms and definitions .5 4 Principle5 5 Test materials.6 6 Sampling.7 7 Test specimens7 8 Procedure .9 9 Validity of the test. 12 10 Expression of results . 12 11 Test report . 13 Annex A (informat
14、ive) Example of a test report 14 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus 16 Annex C (informative) Environmental, health and safety precautions within chemical/biological laboratory 19 Bibliography. 20 EN 1390:2006 (E) 3 Foreword This document (EN 1390:2006) has bee
15、n prepared by Technical Committee CEN/TC 38 “Durability of wood and derived materials”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2006, an
16、d conflicting national standards shall be withdrawn at the latest by December 2006. This document supersedes ENV 1390:1994. Significant technical differences between this standard and ENV 1390:1994 are as follows: a) introduction of new harmonised specifications for the test specimens used in the di
17、verse biological tests; b) separation of the method according to the expected test periods for fast and slow acting preservatives and for deferred acting preservatives respectively; c) admission of the terms given in EN 1001-1and the definitions of EN 1001-2; d) introduction of an informative Annex
18、to take account of consideration for minimisation of environmental and health hazards caused by the use of this biological test. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria,
19、Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EN 1390:2006 (E) 4 Introduct
20、ion This document describes a laboratory method of testing which gives a basis for the assessment of the eradicant action of fast and slow acting wood preservatives and of deferred acting wood preservatives against Hylotrupes bajulus. It allows determination of the lethal effect of a surface applica
21、tion of a preservative product on a population of large larvae previously introduced into the test specimens. The method simulates conditions in practice where a beam is treated, which is only slightly attacked and where cutting away has not exposed insect tunnels. This represents a severe test of t
22、he product. In some particular instances, for example where the preservative is to be used on timbers of large dimensions, laminated beams, blockboard, plywood and other panel products, other test methods can be used to obtain complementary information on the effectiveness of the eradicant action of
23、 a product. Such methods lie outside the scope of this document. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. lt is further recommended that
24、results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products that are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible,
25、 operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel, (see also Annex C for environm
26、ental, health and safety precautions). EN 1390:2006 (E) 5 1 Scope This document specifies a method for the determination of the eradicant action of a surface application of a fast and a slow acting wood preservative product or a deferred acting wood preservative product on timber infested with larva
27、e of Hylotrupes bajulus (Linnaeus). This method is applicable to: organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or water-soluble prod
28、ucts, for example, salts. NOTE An ageing procedure cannot be combined with this method. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition o
29、f the referenced document (including any amendments) applies. EN ISO 3696,Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) ISO 835-1:1981, Laboratory glassware - Graduated pipettes - Part 1: General requirements 3 Terms and definitions For the purposes of this doc
30、ument, the following terms and definitions apply. 3.1 representative sample sample with physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing th
31、e sample of wood preservative to be tested) Adapted from EN 1001-2:2005, 4.83 4 Principle Insertion of larvae of Hylotrupes bajulus into test specimens. After a period of time to allow the larvae to establish themselves in the test specimens, treatment of these test specimens by brushing or pipettin
32、g of the test preservative product. After the time necessary for the preservative to act effectively, assessment of the mortality of the larvae compared with that of larvae in untreated control test specimens. EN 1390:2006 (E) 6 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linn
33、aeus) larvae 5.1.2 Source of larvae. The larvae shall preferably be obtained from cultures reared according to the method described in Annex B. NOTE Larvae can also be taken from naturally infested wood, in which case they should be transferred into sapwood of pine and stored for at least four weeks
34、 under the rearing conditions specified in Annex B. Do not use the larvae in the test if they have not fed normally during this storage period. 5.1.3 Provision of larvae Carefully split or crumble infested blocks to extract larvae. Keep the larvae separate from one another in glass receptacles for t
35、wo or three days in the culturing chamber (5.3.1) to check they are healthy. 5.1.4 Choice of larvae Use only healthy larvae in the test. NOTE 1 A healthy larva can be recognized by ivory-white colour, its firm consistency and rounded appearance, and by the absence of wounds or bites, which show up a
36、s dark marks. Healthy larvae react to the touch by vigorous movement and attempts to bite. Reject any larvae, which are shrunken or aged which have recently moulted, or which are in a pre-pupal stage. Weigh each larva and place it in a glass receptacle marking the receptacle with the weight of the l
37、arva. Make up two groups with the weight ranges: - 51 mg to 100 mg and -101 mg to 150 mg. NOTE 2 Larvae with a mass larger than 150 mg in mass are unsuitable as they can pupate during the course of the test. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens
38、 to be treated with solutions in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found suitable. 5.2.2 Gelatine, for sealing the relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3
39、 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or
40、 other solvents which pose a health risk. EN 1390:2006 (E) 7 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (28 2) C and at a relative humidity of (70 5) %. 5.3.2 Ventilated fume cupboard, in which the specimens are treated with an input air temperature of (20 5) C and a
41、maximum air speed, measured at the input opening with the sash in the approximate operating position, of 0,5 m/s. CAUTION - It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.3 Testing chambe
42、r, ventilated and controlled at (21 2) C and at a relative humidity of (75 5) %. 5.3.4 Drill and twist drills, with 3 mm, 4 mm and 5 mm diameters. 5.3.5 Pipettes as specified in ISO 835-1:1981, Class B - graduated pipette with no waiting time, with a capacity 5 ml and an accuracy of 0,05 ml. 5.3.6 S
43、afety equipment and protective clothing, appropriate for the test product and the test, to ensure the safety of the operator. 5.3.7 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 1 mg. 5.3.8 Rectangular cover with sides, constructed either of glass, plastics
44、, plywood and of a height not less than 200 mm and with an open face of sufficient size to cover all the treated specimens from a single test. 5.3.9 X-ray apparatus (optional) with tungsten target and beryllium window, with voltage and current continuously variable in the ranges: -voltage: 10 kV to
45、50 kV; -current: 0 mA to 15 mA. 5.3.10 Protective gloves 6 Sampling The sample of preservative shall represent the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier. NOTE For the sampling of preservatives from bulk supplies, th
46、e procedure given in EN 212 should be used. 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus)1). NOTE Additional tests may be carried out using other species but, if so, this should be stated in the test report. 1) In southern European countries the
47、 species of pine most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been demonstrated in all aspects (development of larvae, resistance to impregnation, etc.). EN 1390:2006 (E)
48、 8 7.2 Wood quality The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in winter. The wood shall not have been stored for m
49、ore than five years. NOTE 1 Wood that has been kiln dried at temperatures below 60 C may be used. The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole. NOTE 2 It is recommended to use test specimens of a similar growth rate within a single test. 7.3 Provision of test specimens Prepare planed strips having a cross-section of (100 2) mm x (25 2) mm removing a minimum of
