1、Determination of biochemical oxygen demand of water after II days (BOD,) Part 2: Method for undiluted samples (IS0 581 5 : 1989, modified) English version of DIN EN 1899-2 ICs 13.060.01 Supersedes DIN 38409-52, Descriptors: Water quality, biochemical oxygen demand, testing. November 1987 edition DIN
2、 - EN 1899-2 Wasserbeschaffenheit - Bestimmung des Biochemischen Sauerstoff- bedarfs nach y1 Tagen (BSB,) - Teil 2: Verfahren fr unverdnnte Proben (IS0 581 5 : 1989, modifiziert) European Standard EN 1899-2 : 1998 has the status of a DIN Standard. A comma is used as the decimal marker. This standard
3、 is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlamm- untersuchung - Summarische Wirkungs- und Stoffkenngren (Gruppe H) (German standard methods for the examination of water, waste water and sludge - Summary methodes (group H). National foreword This standard has been
4、 prepared by CEN/TC 230. The responsible German body involved in its preparation was the Normenausschu Wasserwesen (Water Practice Standards Committee). Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Depending on the objective of t
5、he analysis, a check shall be made in each individual case on whether and to what extent additional boundary conditions will have to be specified. Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods includ
6、ed in the loose-leaf publication entitled Deutsche Einheitsvetfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by VCH Verlagsgesellschaft and Beuth Verlag GmbH. The standard methods relating to the Wasserhaushaltsgesetz (German Water Management Act), the Abwasserab
7、gabengesetz (Law on the charges leviable for the discharge of effluent into water), and all the regulations on waste waterthat have been issued to date are included in DIN-Taschenbuch (DIN Handbook) 230. Standard methods or draft standards bearing the group title German standard methods for the exam
8、ination of water, waste water and sludge are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory methods (group B) (DIN 38403) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 3840
9、6) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) Biological-ecological methods of analysis (group M) (DIN 3841 O) Microbiological methods (group K) (DIN 3841 1) Continued overleaf. EN c
10、omprises 12 pages. No pari of this standard may be reproduced without the prior permission of V Deutsches Institut fr Normung e. V., Berlin. buth Verlag GmbH, D-10772 Berlin, has the exclusive right of sale for German Standards (DIN-Normen). Ref. No. DIN EN 1899-2 : 1998-0 English price group 07 Sal
11、es No. 1107 10.98 Page 2 DIN EN 1899-2 : 1998-05 Bio-assays (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 3841 4) Bio-assays with microorganisms (group T) (DIN 3841 5) In addition to the methods described in the DIN 38402 to DIN 38415 series
12、of standards, there are a number of European Standards available, which also form part of the collection of German standard methods. Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenausschu Wasserwesen, telephone (O 30)
13、26 O1 -24 23, or from Beufh Verlag GmbH, Burggrafenstrae 6, D-10787 Berlin. Amendments DIN 38409-52, November 1987 edition, has been superseded by the specifications of EN 1899-2. Previous edition DIN 38409-52: 1987-1 1 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 1899-2 March 1998 ICs 13.06
14、0.01 Descriptors: Water quality, biochemical oxygen demand, testing. English version Water quality Determination of biochemical oxygen demand after II days (BOD,) Part 2: Method for undiluted samples (IS0 581 5 : 1989, modified) Qualit de leau - Dtermination de la demande biochimique en oxygne aprs
15、y1 jours (DOB,) - Partie 2: Mthode pour les chantillons non dilus (IS0 581 5 : 1989, modifie) Wasserbeschaffenheit - Bestimmung des Biochemischen Sauerstoff- bedarfs nach y1 Tagen (BSB,) - Teil 2: Verfahren fr unverdnnte Proben (IS0 581 5 : 1989, modifiziert) This European Standard was approved by C
16、EN on 1998-02-22. CEN members are bound to comply with the CENKENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may
17、 be obtained on application to the Central Secretariat or to any CEN member. The European Standards exist in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Centr
18、al Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, the Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, the Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, and the U
19、nited Kingdom. CEN European Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels O 1998. CEN - All rights of exploitation in any form and by any means reserved worldwide for CEN national members. Ref. No.
20、 EN 1899-2 : 1998 E Page 2 EN 1899-2 : 1998 Contents Page Foreword 2 Introduction 3 1 Scope . 3 2 Normative references 4 3 Definition 4 4 Principie . 4 5 Apparatus . 4 7 Procedure 5 8 Calculation and expression of results 6 9 Trueness and Precision . 7 10 Test report . 8 Annex A (normative) Alternat
21、ive incubation periods and temperatures . 9 Annex B (normative) Modifications for specific evaluations 1 1 . 6 Storage of the sample 5 . Annex C (informative 1 Bibliography 12 Foreword This European Standard has been prepared by Technical Committee CEN/TC 230 “Water analysis“, the secretariat of whi
22、ch is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by September 1998, and conflicting national standards shall be withdrawn at the latest by September 1998. According to the CENKENELE
23、C Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switz
24、erland and the United Kingdom. This draft European Standard consists of the following Parts: EN 1899-1 Water quality - Determination of biochemical oxygen demand after n days (BOD,) - Part 1: Dilution and seeding method with allylthiourea addition Water quality - Determination of biochemical oxygen
25、demand after n days (BOD,) - Part 2: Method for undiluted samples EN 1899-2 Annex A, which is normative, concerns alternative incubation periods. Annex B, which is normative, concerns modifications for specific evaluations of the quality of waters. Annex C, which is informative, contains bibliograph
26、y. Endorsement notice The text of the International Standard IS0 5815:1989 was approved by CEN as a European Standard with agreed common modifications as given below: - Change in setup (splitting into two parts) - Additions of annexes. Page 3 EN 1899-2 : 1998 Introduction This European Standard EN 1
27、899-2 is intended for analysis of BOD in waters with a BOD in the range (0,5 to 6) mg/l of oxygen. The times of incubation specified in the proposal are 5 days - as in IS0 581 5: 1989, “Water Quality - Deter- mination of biochemical oxygen demand after 5 days (BOD,) - Dilution and seeding method“. I
28、ncubation for 5 days - as has been applied in many European countries - or 7 days as has been applied in several Nordic countries through the past many years. The 7 day incubation will typically give higher BOD results than 5 days incubation time. With an incubation time of 5 days weekend work can o
29、nly be avoided, if samples are collected Wednesdays, Thursdays to Fridays. With an incubation time of 7 days, samples collected on the first five weekdays can be analysed without implying weekend work. For this reason a 7 day incubation can be considered more convenient than the conventional 5 day i
30、ncubation. A modified 7 day incubation is described in a normative annex. The first investigations indicate that BOD results obtained by this modified method are identical to results obtained by the 5 day method described in the proposed standard. It is hoped that more comparative data on these two
31、incubation methods will be obtained during the coming years, so that the modified 7 day incubation method can be included fully at the time of review and revision of this standard. 1 Scope This European Standard specifies determination of the biochemical oxygen demand of waters of undiluted samples.
32、 This standard is applicable to all waters having biochemical oxygen demands greater than or equal to the limit of determination 0,5 mg/l of oxygen and not exceeding 6 mg/l of oxygen. In this standard, the limit of determination, D, is defined as .$ + n DL = 0,QOV) . . B where sB is the within serie
33、s standard deviation, is the Student t-value, with f is the degrees of freedom for the determination of s, and n is the number of analysis for determination of the blank in an analytical series. s, is calculated from determinations of real samples with a BOD contentration near the estimated O,. In c
34、ases where the analytical method does not require any blank correction, the term is omitted. The results obtained are the product of a combination of biochemical and chemical reactions. They do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defin
35、ed, chemical process. Nevertheless, they provide an indication from which the quality of waters can be estimated. The test can be influenced by the presence of various substances. Those which are toxic to microorganisms, for example bactericides, toxic metals or free chlorine, will inhibit biochemic
36、al oxidation. The presence of algae or nitrifying microorganisms can produce high results. In these situations a modification of the method (see annex B or as described in EN 1899-1. It is absolutely essential that test conducted according to this standard are carried out by suitably qualified staff
37、. Annex A describes alternative incubation periods. Annex B describes procedures for modification of the method by addition of seeding material, salts, inhibition of nitrification by allylthiourea (ATU) addition, neutralisation, homogenisation and/or filtration. These modifica- tions may be found ne
38、cessary for specific evaluations of the water quality of receiving waters. Page 4 EN 1899-2 : 1998 2 Normative references This European Standard incorporates by dated or undated reference provisions from other publications. These normative references are cited at the appropriate places in the text a
39、nd the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies.
40、EN IS0 3696 Water for analytical laboratory use - Specification and test methods (IS0 3696:1987) EN 25813 Water quality - Determination of dissolved oxygen - lodometric method (IS0 581 3:1983) EN 25814 Water quality - Determination of dissolved oxygen - Electrochemical probe method (IC0 581 4: 1990)
41、 IS0 6107-2:1997 Water quality - Vocabulary - Part 2 3 Definition For the purposes of this European Standard, the following definition applies: biochemical oxygen demand after n days (BOD,): The mass concentration of dissolved oxygen consumed under specified conditions by the .biochemical oxidation
42、of organic and/or inorganic matter in water. n is the incubation time; it IS equal to 5 or 7. NOTE: For the purpose of this European Standard, “biochemical oxidation“ is taken to mean “biological oxidation“. This definition is similar to the definition of IS0 6107-2. 4 Principle Equilibration of the
43、 sample of water to be analyzed to 20 OC followed by, if necessary, aeration. Incubation at 20 OC for a defined period, 5 or 7 days, in the dark, in a completely filled and stoppered bottle. Determination of the dissolved oxygen concentration before and after incubation. Calculation of the mass of o
44、xygen consumed per litre of sample. 5 Apparatus 5.1 Incubation bottles, BOD bottles, with glass stoppers, For example, preferably 250 ml to 300 ml or 1 O0 ml to 125 ml, with stoppers and preferably with straight shoulders, or any equivalent bottles. It is important that the bottles are thoroughly cl
45、eaned before use. If the iodornetric method (in accordance with EN 2581 3) for determining dissolved oxygen is used, it is, for example, normally sufficient to rinse the bottle several times with tap water then deionised water. However, if the electrode method (in accordance with EN 25814) is used,
46、a more stringent cleaning procedure, for example, as follows, is required. Add to the empty bottle 5 ml to 10 ml of a wash solution (for example 2,5 g of iodine plus 12,5 g of potassium iodide per litre of 1 % (V/W sulfuric acid) shaking well to coat the bottle walls. Let stand for 15 min, pour off
47、the solution and rinse thoroughly with tap water and finally deionised water. 5.2 Incubator, capable of being maintained at (20 f 1) OC. 5.3 Equipment for determining dissolved oxygen concentration In accordance with EN 2581 3 and EN 2581 4. 5.4 Means of refrigeration, (O to 4) OC, for transport and
48、 storage of the sample. 5.5 Aeration equipment A bottle of compressed air or a compressor: The air quality shall be such that the aeration does not lead to any contaminations, especially by the addition of organic matter, oxidizing or reducing materials, or metals. If a contamination is suspected, t
49、he air shall be filtered or washed. Page 5 EN 1899-2 : 1998 6 Storage of the sample Store the sample at a temperature (O to 4) OC in a filled and hermetically stoppered bottle immediately after sample collection and until the analysis is performed. Begin the determination of the BOD, as soon as possible and within 24 h after completion of sample collection. 7 Procedure 7.1 Preparation of test solutions Bring the test sample to a temperature of (20 f 2) OC and aerate if necessary. In case of aeration, let the sample stand about 15 min. Remove air bubbles and possi
