1、October 2015 Translation by DIN-Sprachendienst.English price group 12No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).IC
2、S 71.100.50!%FmZ“2357455www.din.deDIN EN 49-2Wood preservatives Determination of the protective effectiveness against Anobium punctatum (De Geer) by egglaying and larval survival Part 2: Application by impregnation (Laboratory method);English version EN 492:2015,English translation of DIN EN 49-2:20
3、15-10Holzschutzmittel Bestimmung der vorbeugenden Wirkung gegenber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Larven Teil 2: Anwendung durch Volltrnkung (Laboratoriumsverfahren);Englische Fassung EN 492:2015,Englische bersetzung von DIN EN 49-2:2015-10Produits de
4、 prservation du bois Dtermination de lefficacit protectrice vis vis de Anobium punctatum (De Geer) par lobservation de la ponte et de la survie des larves Partie 2: Application par imprgnation (Mthode de laboratoire);Version anglaise EN 492:2015,Traduction anglaise de DIN EN 49-2:2015-10SupersedesDI
5、N EN 492:200506www.beuth.deIn case of doubt, the German-language original shall be considered authoritative.DDocument comprises 23 pages09.15DIN EN 49-2:2015-10 2 A comma is used as the decimal marker. National foreword This document (EN 49-2:2015) has been prepared by Technical Committee CEN/TC 38
6、“Durability of wood and wood-based products” (Secretariat: AFNOR, France). The responsible German body involved in its preparation was the DIN-Normenausschuss Holzwirtschaft und Mbel (DIN Standards Committee Timber and Furniture), Working Committee NA 042-03-06 AA Spiegel-ausschuss zu CEN/TC 38 und
7、ISO/TC 165/SC 1 Dauerhaftigkeit von Holz und Holzprodukten. Amendments This standard differs from DIN EN 49-2:2005-06 as follows: a) the materials for preparing the egg-laying zones have been described in a generalized way; b) new harmonized specifications for wood quality have been included. Previo
8、us editions DIN EN 49 1977-03 DIN EN 49-2: 1992-11, 2005-06 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 49-2 August 2015 ICS 71.100.50 Supersedes EN 49-2:2005English Version Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying
9、and larval survival - Part 2: Application by impregnation (Laboratory method) Produits de prservation du bois - Dtermination de lefficacit protectrice vis vis de Anobium punctatum (De Geer) par lobservation de la ponte et de la survie des larves - Partie 2 : Application par imprgnation (Mthode de la
10、boratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Larven -Teil 2: Anwendung durch Volltrnkung (Laboratoriumsverfahren) This European Standard was approved by CEN on 16 July 2015. CEN members are
11、bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to th
12、e CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Cent
13、re has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuani
14、a, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-100
15、0 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 49-2:2015 EEN 49-2:2015 (E) 2 Contents Page European foreword .3 Introduction .4 1 Scope 5 2 Normative reference 5 3 Terms and definitions .5 4 Principle 5 5 Test mate
16、rials .6 5.1 Biological material .6 5.2 Products and reagents 6 5.3 Apparatus .6 6 Sampling .7 7 Test specimens 8 7.1 Species of wood.8 7.2 Wood quality 8 7.3 Provision of test specimens .8 7.4 Dimensions of test specimens .8 7.5 Number of test specimens 8 8 Procedure .9 8.1 Preparation of the test
17、specimens .9 8.1.1 Conditioning of test specimens before treatment 9 8.1.2 Treatment of the test specimens 9 8.1.3 Drying and conditioning of the test specimens after treatment 10 8.2 Exposure of the test specimens to the insects . 11 8.3 Conditions and duration of the test 11 8.4 Examination of the
18、 test specimens . 11 9 Validity of test . 12 10 Expression of results . 12 10.1 Assessment of the protective effectiveness 12 10.2 Toxic values 12 11 Test report . 12 Annex A (informative) Example of a test report 14 Annex B (informative) Identification of sex of test insects (Anobium punctatum) . 1
19、6 Annex C (informative) Culturing technique for Anobium punctatum . 17 Annex D (informative) Environmental, health and safety precautions within chemical/biological laboratory 20 Bibliography . 21 DIN EN 49-2:2015-10 EN 49-2:2015(E)3 European foreword This document (EN 49-2:2015) has been prepared b
20、y Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by February 2016 and conflicti
21、ng national standards shall be withdrawn at the latest by February 2016. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document
22、 supersedes EN 49-2:2005. Significant technical differences between this document and EN 49-2:2005 are as follows: a) generalization of material for preparing the egg-laying zones; b) introduction of new harmonized specifications for wood quality. EN 49, Wood preservatives Determination of the prote
23、ctive effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival, consists of two parts: Part 1: Application by surface treatment (Laboratory method); Part 2: Application by impregnation (Laboratory method). EN 49-1 is required to enable effectiveness assessments of wood pre
24、servatives which are intended to be applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austr
25、ia, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden
26、, Switzerland, Turkey and the United Kingdom. DIN EN 49-2:2015-10 EN 49-2:2015 (E) 4 Introduction This document describes a laboratory method of testing which gives a basis for assessment of the effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the conce
27、ntration at which the product prevents the development of infestation from egg laying. The method simulates conditions which can occur in practice on timber which has been treated some time previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid. This l
28、aboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further recommended that results from this test should be supplemented by those from other appro
29、priate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood,
30、 laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). DIN EN 49-2:2015-10 EN 49-2:2015
31、 (E) 5 1 Scope This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method
32、is applicable to: water-insoluble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates,
33、 and water-soluble materials, for example salts. NOTE This method can be used in conjunction with an ageing procedure, for example EN 73. 2 Normative reference The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dat
34、ed references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this docum
35、ent, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled 3.2 supplier sponsor of the test (person or company providing the sample of wood pres
36、ervative to be tested) 4 Principle The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid, the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in untreated control test specimens. If the preservative has been p
37、repared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens. Depending on the test being carried out either on a set of test specimens of a susceptible wood species that is imp
38、regnated with a solution of the preservative, or DIN EN 49-2:2015-10 EN 49-2:2015 (E) 6 if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are impregnated with a series of solutions in which the concentration of preservative is ranged in a give
39、n progression. 5 Test materials 5.1 Biological material Anobium punctatum (De Geer) Adult males and females in good condition. Adults to be used in the test shall be collected daily from naturally infested wood or laboratory culture (see Annex C). Use recently emerged adults which have been recently
40、 collected; kept overnight in quarantine (see C.2.2 and C.6) and then checked to ensure that they are undamaged, active, and free from any infestation by mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and females in separate containers. NOTE The propor
41、tion of males and females varies during the emergence period. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the end sections of test specimens. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Paste, for securing filter paper. The paste shall be
42、 starch-free, non-toxic to Anobium punctatum and insoluble in the product under test. NOTE Sodium carboxymethyl cellulose, food grade, has been found to be suitable. 5.2.3 Xylene, technical grade, mixed isomers. 5.2.4 Water, complying with grade 3 of EN ISO 3696. 5.2.5 Solvent or diluent, a volatile
43、 liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.2.6 Filter paper, ordinary quality, med
44、ium-fast grade. 5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the egg-laying zones. NOTE Cotton, linen and polyamide-gauze have been proven suitable. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (21 2) C, and at
45、relative humidity (80 5) %. 5.3.2 Conditioning chamber, well ventilated, controlled at (20 2) C and at relative humidity (65 5) %. DIN EN 49-2:2015-10 EN 49-2:2015 (E) 7 The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has the condition
46、s specified for the conditioning chamber (see 5.3.2). 5.3.3 Treatment vessel(s), of a material that does not react with the preservative under test, for example of glass for organic products and of polyethylene for salts containing fluorine. 5.3.4 Laboratory work area, well ventilated, where treatme
47、nt of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
48、 5.3.6 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-fitting cover and containing supports that will give minimum contact with treated test specimens to be placed on them. The vessels and supports shall be of a material that does not react with the pre
49、servative under test, for example glass for organic compounds and polyethylene for products containing fluorine. 5.3.7 Vacuum vessel(s), fitted with stopcocks, capable of receiving the treatment vessels (5.3.3). 5.3.8 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa1). 5.3.9 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test. 5.3.10 Safety equipment and protective clothing
