1、December 2015 English price group 32No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.060.70!%J-b“2391063www.din.d
2、eDIN EN 16695Water quality Guidance on the estimation of phytoplankton biovolume;English version EN 16695:2015,English translation of DIN EN 16695:2015-12Wasserbeschaffenheit Anleitung zur Abschtzung des Phytoplankton-Biovolumens;Englische Fassung EN 16695:2015,Englische bersetzung von DIN EN 16695:
3、2015-12Qualit de leau Lignes directrices pour lestimation du biovolume des microalgues;Version anglaise EN 16695:2015,Traduction anglaise de DIN EN 16695:2015-12www.beuth.deDocument comprises 10 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be considere
4、d authoritative.411.15DIN EN 16695:2015-12 2 A comma is used as the decimal marker. National foreword This document (EN 16695:2015) has been prepared by Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was DIN-Normen
5、ausschuss Wasserwesen (DIN Standards Committee Water Practice), Working Committee NA 119-01-03-05-06 AK Biologisch-kologische Gewsseruntersuchung of NA 119-01-03 AA Wasseruntersuchung. Designation of the method: Guidance on the estimation of phytoplankton biovolume: Method DIN EN 16695 M 37 This sta
6、ndard has been prepared under mandate M/424, which was given to CEN by the European Commission for the development and enhancement of standard methods in support of the Water Framework Directive (WFD) (Directive 2000/60/EC). DIN EN 16695:2015-12 3 Expert assistance and specialized laboratories will
7、be required to perform the analyses described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what extent additional conditions will have to be specified. This standard h
8、as been prepared by DIN-Normenausschuss Wasserwesen (DIN Standards Committee Water Practice) in collaboration with the Wasserchemische Gesellschaft null Fachgruppe in der Gesellschaft Deutscher Chemiker (Water Chemistry Society Division of the German Chemical Society). It is part of the series Deuts
9、che Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung (German standard methods for the examination of water, waste water and sludge): Guidance on the estimation of phytoplankton biovolume (M 37). Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either
10、 individually or grouped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Beuth Verlag GmbH and Wiley-VCH Verlag GmbH hd Examples: Aphanizomenon, Aulacoseira, Co
11、scinodiscus, Phormidium, Rhizosolenia, Thalassiosira = 214V dh For radius =2dr : =2V rh 5 Elliptic cylinder prism on elliptic base oval cylinder cross section longitudinal sections basic shape Volume calculation precision: exact Dimensions: large diameter (d1) - m small diameter (d2) - m; hd height
12、of cylinder (h) - hd; m Examples: Achnanthes, Chaetoceros, Fragilaria, Odontella, Surirella = 1214V ddh For radius r: =12V rrh with =112dr ; =222dr DIN EN 16695:2015-12 EN 16695:2015 (E)17 ID Geometrical shape (Synonyms) Information Volume formula 6 Lanceolate cylinder naviculoid cross section longi
13、tudinal sections basic shape Volume calculation precision: approximate Dimensions: large diameter (d1) - m small diameter (d2) - m; hd height of cylinder (h) - hd; m Examples: Haslea, Navicula =122V ddh For radius r: =128V rrh with =112dr ; =222dr 7 Cone cross section longitudinal section basic shap
14、e Volume calculation precision: exact Dimensions: diameter (d) - m slant height (s) - m height of cone (z) - hd NOTE With an inverted microscope, the slant height can exactly be measured. The cone height is a hidden dimension because of the oblique projection. Examples: Calciopappus, Minuscula, Pyra
15、mimonas longicauda = 22 21112 4V ds d For radius =2dr and height = 22z sr: = 213V rz DIN EN 16695:2015-12 EN 16695:2015 (E) 18 ID Geometrical shape (Synonyms) Information Volume formula 8 Double cone two cones cross section longitudinal section composed shape basic shape: cone Volume calculation pre
16、cision: exact Dimensions: diameter (d) - m height of double cone (z) - m NOTE Due to the position in the sedimentation chamber, the total height can only be measured approximately because of the oblique projection. Examples: Gyrodinium spirale, Heterocapsa triquetra, Protoperidinium crassipes = 2112
17、V dz For radius =2dr : = 211232V rz 9 Cuboid rectangular box rectangular parallelepiped cross section longitudinal sections basic shape Volume calculation precision: exact Dimensions: first edge length (a) - m second edge length (b) - m; hd third edge length (c) - hd; m Examples: Bacillaria, Hantzsc
18、hia, Pinnularia, Tabellaria =V abc DIN EN 16695:2015-12 EN 16695:2015(E)19 ID Geometrical shape (Synonyms) Information Volume formula 10 Rhombic prism parallelepiped prism on parallelogram base cross section longitudinal sections basic shape Volume calculation precision: exact Dimensions: large diag
19、onal (d1) - m small diagonal (d2) - m; hd height of prism (h) - hd; m Examples: Brachysira, Nitzschia, Pseudo-nitzschia =1212V ddh 11 Trapezoid prism cross section longitudinal section basic shape Volume calculation precision: exact Dimensions: length of large trapezoid side (l1) - m length of small
20、 trapezoid side (l2) - m height of trapezoid (z) - m; hd height of prism (h) - hd; m Examples: Crucigenia fenestrata ( )= + 1212V l l zh DIN EN 16695:2015-12 EN 16695:2015 (E) 20 ID Geometrical shape (Synonyms) Information Volume formula 12 Triangular prism prism on triangle base cross section longi
21、tudinal section basic shape Volume calculation precision: exact Dimensions: length of triangle side (l) - m height of triangle (m) - m; hd height of prism (h) - hd; m NOTE For a prism on basis of an equilateral triangle, only the triangle side is needed. The triangle height can be expressed by this
22、side. Examples: Bellerochea, Ditylum, Goniochloris, Lithodesmium, Triceratium = 12V lmh or = 2134V lh for a prism on basis of an equilateral triangle with =132ml 13 Pyramid Cross sections Top view basic shape Volume calculation precision: exact Dimensions: length of first basic side (l1) - m length
23、of second basic side (l2) - m; hd height of pyramid (h) - hd; m Examples: Gomphonema, Licmophora =1213V llh DIN EN 16695:2015-12 EN 16695:2015 (E) 21 ID Geometrical shape (Synonyms) Information Volume formula 14 Tetrahedron lateral view top view basic shape Volume calculation precision: exact Dimens
24、ions: edge length (a) - m height of tetrahedron (h) hd Examples: Staurastrum, Staurodesmus, Tetraedriella, Tetraedron =21312V ah or =31212Va for a regular tetrahedron with =163ha; 15 Spindle cross section longitudinal section basic shape Volume calculation precision: approximate Dimensions: diameter
25、 (d) - m height (h) - m Examples: Ankistrodesmus, Closterium, Monoraphidium = 2215V dh For radius =2dr : = 2815V rh DIN EN 16695:2015-12 EN 16695:2015(E)22 ID Geometrical shape (Synonyms) Information Volume formula 16 Cone with half sphere cone with hemisphere cross section longitudinal section comp
26、osed shape basic shapes: cone, sphere Volume calculation precision: exact Dimensions: diameter (d) - m total height (h) - m NOTE Due to the position in the sedimentation chamber, the total height can only be measured approximately because of the oblique projection. Examples: Gonyaulax, Hemiselmis, K
27、atodinium, Teleaulax = +21112 2V dh d For radius =2dr : ( )= +231 143 23V r hr r17 Ceratium-shape lateral view Complicated composed shape Volume calculation precision: approximate Dimensions: girdle diameter (d) - m Examples: Ceratium = yV xd x and y are factors derived from measurements 4, 9 Cerati
28、um x: 0,32359 y: 2,9953 C, furca x: 2,3038 y: 2,532 C, fusus x: 35,198 y: 1,9156 C, hirundinella x: 2,3038 y: 2,532 C, horridum x: 0,32437 y: 3,0474 C, lineatum x: 1,2375 y: 2,5989 C, longipes x: 0,32437 y: 3,0474 C, macroceros x: 0,32437 y: 3,0474 C, tripos x: 0,32359 y: 2,9953 DIN EN 16695:2015-12
29、 EN 16695:2015(E)23 Annex B (informative) Performance data The performance data given in Table B.1 to Table B.4 were determined in a European interlaboratory comparison for validation carried out in March 2014 on two mixed marine and two freshwater samples taken from the North Sea (Helgoland, German
30、y), the Baltic Sea (Stralsund, Germany) and different lakes around Oldenburg (Germany) and Bad Saarow (Germany). 52 participants from the following European countries have provided results: Denmark (4), Finland (3), France (1), Germany (20), Ireland (1), Italy (3), Lithuania (3), Norway (1), Poland
31、(2), Portugal (3), Spain (2), Sweden (3) and United Kingdom (6). All results have been calculated according to the German standard document DIN 38402-45:2014-06. DIN EN 16695:2015-12 EN 16695:2015(E)24 Table B.1 Performance data for measurements of first dimension in biovolume analysis of phytoplank
32、ton species in natural samples - Abbreviations for dimensions according to Annex A. Taxon name l n X xmedxmeanxHSsRCV,RsrCV,rm m m m m % m % Dactyliosolen fragilissimus (d) 45 1 225 11,74 11,93 12,06 12,04 2,01 16,7 1,80 14,9 Dinophysis acuminata (d1) 42 961 33,48 33,90 34,07 34,07 4,24 12,5 3,23 9,
33、5 Ceratium tripos (d1) 44 998 66,79 65,93 66,74 66,66 6,12 9,2 4,56 6,8 Pseudo-nitzschia cf.pungens (d1) 44 1 054 111,87 111,69 111,12 111,19 12,43 11,2 11,18 10,1 Thalassionema nitzschioides (d1) 42 1 013 32,95 32,82 33,15 33,15 4,29 13,0 4,25 12,8 Rhizosolenia imbricata (d) 42 1 154 9,68 10,19 10,
34、16 10,19 2,49 24,4 2,14 21,0 Ditylum brightwellii (l) 40 1 114 26,76 23,83 23,64 23,69 4,89 20,7 4,18 17,6 Stephanopyxis turris (d) 37 964 43,15 40,35 39,96 39,96 7,60 19,0 7,07 17,7 Odontella sinensis (d1) 40 1 106 167,28 163,87 175,38 174,21 40,62 23,3 33,02 19,0 Chaetoceros debilis (d1) 39 982 13
35、,39 14,25 13,65 13,65 3,84 28,2 2,77 20,3 Fragilaria crotonensis (d1) 53 1 391 76,94 76,58 78,15 77,47 12,51 16,2 14,27 18,4 Tabellaria fenestrata (a) 47 1 052 51,62 50,91 50,77 50,77 4,77 9,4 4,20 8,3 Aulacoseira granulata (d) 50 1 183 5,48 5,88 5,89 5,90 1,02 17,3 0,84 14,2 Cryptomonas erosa (d1)
36、50 1 133 12,63 12,83 12,96 12,96 1,58 12,2 1,32 10,2 Rhodomonas lacustris (d) 49 1 249 5,20 5,27 5,28 5,28 0,66 12,5 0,71 13,5 Planktothrix agardhii (d) 47 1 260 3,32 3,34 3,44 3,42 0,72 21,0 0,15 4,3 Monoraphidium arcuatum (d) 42 1 085 1,73 1,69 1,83 1,78 0,54 30,3 0,32 17,8 Woronichinia naegeliana
37、 (d) 44 1 184 2,92 3,27 3,26 3,26 0,60 18,3 0,39 12,0 Cosmarium ocellatum (d1) 43 977 28,60 26,21 26,44 26,46 2,54 9,6 1,85 7,0 Trachelomonas hispida (d) 45 1 068 21,15 20,96 20,86 20,87 2,08 10,0 1,75 8,4 Explanation of symbols l number of laboratories with |zu-score| 3 n number of total individual
38、 test results of all laboratories with |zu-score| 3 X assigned reference value based on 7 replicates with each having a minimum of 30 single measurements xmed interlaboratoryamedian value xmean interlaboratoryaarithmetic mean value xHS interlaboratoryarobust mean value (Hampel Schaetzer) sR reproduc
39、ibility standard deviation CV,R relative reproducibility standard deviation sr repeatability standard deviation CV,r relative repeatability standard deviation aBased on arithmetic means of individual laboratory measurements; only laboratories with |zu-score| 3 included. DIN EN 16695:2015-12 EN 16695
40、:2015 (E) 25 Table B.2 Performance data for measurements of second dimension in biovolume analysis of phytoplankton species in natural samples - Abbreviations for dimensions according to Annex A. Taxon name l n X xmedxmeanxHSsRCV,RsrCV,rm m m m m % m % Dactyliosolen fragilissimus (h) 45 1 225 60,61
41、59,69 59,32 59,32 12,01 20,3 11,86 20,0 Dinophysis acuminata (d2) 43 994 20,09 19,45 20,58 20,32 5,58 27,5 1,90 9,4 Ceratium tripos (d2) 45 1 018 43,41 38,00 38,53 38,09 11,82 31,0 2,12 5,6 Pseudo-nitzschia cf.pungens (d2) 43 1 024 4,81 4,51 4,59 4,58 1,01 22,0 0,84 18,4 Thalassionema nitzschioides
42、(d2) 42 1 013 3,99 4,35 4,26 4,23 0,99 23,4 0,77 18,2 Rhizosolenia imbricata (h) 42 1 154 188,20 201,34 200,16 200,46 45,13 22,5 41,59 20,7 Ditylum brightwellii (m) 40 1 114 23,17 22,99 22,87 22,96 4,47 19,5 4,51 19,7 Stephanopyxis turris (h) 37 964 66,83 59,20 58,30 58,30 10,36 17,8 9,62 16,5 Odont
43、ella sinensis (d2) 40 1 106 66,91 64,27 64,17 63,63 20,62 32,4 11,50 18,1 Chaetoceros debilis (d2) 38 959 10,04 9,31 9,53 9,40 2,59 27,6 1,59 16,9 Fragilaria crotonensis (d2) 47 1 171 4,40 3,96 3,99 3,98 0,96 24,2 0,23 5,9 Tabellaria fenestrata (b) 47 1 052 5,16 7,57 7,39 7,41 2,33 31,4 1,07 14,4 Au
44、lacoseira granulata (h) 43 966 27,33 26,04 26,11 26,11 3,16 12,1 2,68 10,3 Cryptomonas erosa (d2) 49 1 113 10,73 10,13 9,95 9,99 2,10 21,1 0,95 9,5 Rhodomonas lacustris (h) 49 1 249 11,43 10,53 10,61 10,61 1,85 17,4 1,67 15,7 Planktothrix agardhii - - - - - - - - - - Monoraphidium arcuatum (h) 41 1
45、065 49,31 42,00 41,25 41,25 7,92 19,2 4,98 12,1 Woronichinia naegeliana (h) 43 1 077 5,35 5,38 5,33 5,36 0,69 12,9 0,49 9,2 Cosmarium ocellatum (d2) 43 956 14,30 13,39 13,52 12,19 2,52 20,7 0,87 7,2 Trachelomonas hispida (h) 45 1 068 27,12 27,13 26,83 26,80 3,01 11,2 2,35 8,8 For explanation of symb
46、ols, see Table B.1. DIN EN 16695:2015-12 EN 16695:2015 (E) 26 Table B.3 Performance data for measurements of third dimension in biovolume analysis of phytoplankton species in natural samples - Abbreviations for dimensions according to Annex A Taxon name l n X xmedxmeanxHSsRCV,RsrCV,rm m m m m % m %
47、Dactyliosolen fragilissimus - - - - - - - - - - Dinophysis acuminata (h) 42 961 46,69 48,71 48,88 48,77 5,38 11,0 4,58 9,4 Ceratium tripos (h1) 42 938 40,33 42,67 41,39 41,44 6,27 15,1 3,34 8,1 Pseudo-nitzschia cf.pungens (h) 44 1 054 4,81 4,06 4,14 4,12 1,51 36,7 0,66 16,1 Thalassionema nitzschioid
48、es (h) 42 1 013 4,59 4,69 4,77 4,76 1,00 21,0 0,78 16,4 Rhizosolenia imbricata - - - - - - - - - - Ditylum brightwellii (h) 40 1 114 74,17 70,10 71,00 70,88 17,30 24,4 17,01 24,0 Stephanopyxis turris - - - - - - - - - - Odontella sinensis (h) 40 1 106 195,30 180,70 175,04 175,34 47,20 26,9 42,85 24,
49、4 Chaetoceros debilis (h) 38 954 9,14 8,83 8,99 8,91 2,10 23,6 1,57 17,6 Fragilaria crotonensis (h) 49 1 291 4,40 3,76 3,48 3,48 0,98 28,2 0,30 8,6 Tabellaria fenestrata (c) 41 929 7,82 4,88 5,47 5,40 2,06 38,2 0,70 13,0 Aulacoseira granulata - - - - - - - - - - Cryptomonas erosa (h) 49 1 113 25,85 25,75 25,91 25,91 3,64 14,1 3,53 13,6 Rhodomonas lacustris - - - - - - - - - - Planktothrix agardhii - - - - - - - - - - Monoraphidi
