1、May 2009DEUTSCHE NORM English price group 15No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.060.70!$W_4“1526017www.
2、din.deDDIN EN ISO 11348-1Water quality Determination of the inhibitory effect of water samples on the lightPart 1: Method using freshly prepared bacteria (ISO 11348-1:2007)English version of DIN EN ISO 11348-1:2009-05Wasserbeschaffenheit Teil 1: Verfahren mit frisch gezchteten Bakterien (ISO 11348-1
3、:2007)Englische Fassung DIN EN ISO 11348-1:2009-05SupersedesDIN EN ISO 11348-1:1999-04www.beuth.deemission of Vibrio fischeri (Luminescent bacteria test) Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission von Vibriofischeri (Leuchtbakterientest) Document comprises 33 pagesDIN EN ISO 1
4、1348-1:2009-05 This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung Testverfahren mit Wasserorganismen (Gruppe L) (German standard methods for the examination of water, waste water and sludge Test methods using water organisms (group L) and de
5、scribes method L 51. National foreword This standard has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was the Normenausschuss
6、Wasserwesen (Water Practice Standards Committee), Technical Committees NA 119-01-03-05-01 AK Biotests and NA 119-01-03 AA Wasseruntersuchung. This standard has been published to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 Oc
7、tober 2000 establishing a framework for Community action in the field of water policy1). ISO 11348 consists of the following parts, under the general title Water quality Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test): Part
8、 1: Method using freshly prepared bacteria Part 2: Method using liquid-dried bacteria Part 3: Method using freeze-dried bacteria Designation of method L 51 “Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) Part 1: Method usi
9、ng freshly prepared bacteria”: Method DIN EN ISO 11348-1 L 51 Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Existing safety regulations are to be taken into account. Depending on the objective of the analysis, a check shall be mad
10、e on a case-by-case basis as to whether and to what extent additional conditions will have to be specified. Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods included in the loose-leaf publication entitl
11、ed Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth Verlag GmbH. 1)Registered in the DITR database of DIN Software GmbH, available from: Beuth Verlag GmbH, 10772 Berlin. 2 DIN EN ISO 11348-1:2009-05 All standard met
12、hods relevant to the Abwasserverordnung (Waste Water Regulation) (AbwV) included in the new Regulation on Section 7a of the Gesetz zur Ordnung des Wasserhaushaltes (German Water Management Act) concerning Anforderungen an das Einleiten von Abwasser in Gewsser together with the Abwasserverordnung and
13、 the Gesetz zur Ordnung des Wasserhaushalts have been published by Beuth Verlag GmbH as a loose-leaf collection Analysenverfahren in der Abwasserverordnung Rechtsvorschriften und Normen (Supplements). Standard methods or draft standards bearing the group title “German standard methods for the examin
14、ation of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory analysis (group B) (DIN 38403) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 3840
15、6) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) Biological-ecological methods of analysis (group M) (DIN 38410) Microbiological methods (group K) (DIN 38411) Test methods using water o
16、rganisms (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) In addition to the methods described in the DIN 38402 to DIN 38415 series of standards, there are a number of European and Inte
17、rnational Standards available as DIN EN, DIN EN ISO and DIN ISO Standards, which also form part of the collection of German standard methods. Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenausschuss Wasserwesen, teleph
18、one +49 30 2601-2448, or from Beuth Verlag GmbH, Burggrafenstr. 6, 10787 Berlin. The DIN Standards corresponding to the International Standards referred to in the EN are as follows: ISO 5667-16 DIN EN ISO 5667-16 ISO 5814 DIN EN 25814 ISO 7027 DIN EN ISO 7027 ISO 10253 DIN EN ISO 10253 3 DIN EN ISO
19、11348-1:2009-05 Amendments This standard differs from DIN EN ISO 11348-1:1999-04 as follows: a) The scope has been extended to include: sea water; sediment eluates (fresh water, brackish and sea water); single substances, diluted in water. b) Clause 4 “Interferences” has been revised: the oxygen con
20、centration of readily biodegradable nutrients is no longer specified; reference is now made to Bibliography 1; specifications regarding interferences due to samples with extreme pH values have been included; when testing salt-water samples, possible inferences as a result of stimulation effects of b
21、ioluminescence are to be taken into account. c) Clause 5 “Reagents and materials”: information on sources of supply for luminescence bacteria has been included; the concentration of the 3,5-Dichlorophenol reference substance has been changed and the purity has been specified; commercially available
22、reference substance solutions may now be used. d) Modifications relating to the preparation of the procedure: apparatus: the permissible temperature deviation within one test has been changed from 0,2 C to 0,3 C; sampling: samples may now be stored at 18 C for periods of up to two months (instead of
23、 two weeks); sample preparation: the oxygen concentration is to be measured in accordance with ISO 5814 and samples having an oxygen concentration of less than 3 mg/l are to be oxygenated by aeration or stirring; preparation of pre-cultures: incubation time for pre-cultures has been reduced to 3 day
24、s (instead of 5 days); preparation of stock suspension: the aliquot volume of the bacterial suspension in the German version has been corrected to read 100 l (instead of 100 l); freezing of the stock suspension for future use is mandatory and the potential storage period has been extended. e) Modifi
25、cations relating to the procedure: two parallel determinations have been specified for the tests; the test suspension is prepared now in two steps. f) A further method for the calculation of the concentration-effect relationship has been included (e.g. as in ISO/TS 20281). g) Modifications relating
26、to the criteria of validity: the permissible upper limit for the tfk-value has been reduced to 1,3 (formerly 1,8). h) In Annex B, the volumes of the test and control batches have been modified and a dilution scheme has been included. i) Annex D “Testing salt water samples with the luminescent bacter
27、ia test with freshly cultured bacteria” has been added. j) The standard has been editorially revised. 4 DIN EN ISO 11348-1:2009-05 Previous editions DIN 38412-34: 1991-03, 1997-07 DIN EN ISO 11348-1: 1999-04 National Annex NA (informative) Bibliography DIN EN 25814, Water quality Determination of di
28、ssolved oxygen by the electrochemical probe method DIN EN ISO 5667-16, Water quality Sampling Part 16: Guidance on biotesting of samples DIN EN ISO 7027, Water quality Determination of turbidity DIN EN ISO 10253, Water quality Marine algal growth inhibition test with Skeletonema costatum and Phaeoda
29、ctylum tricornutum 5 DIN EN ISO 11348-1:2009-05 6 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11348-1 November 2008 ICS 13.060.70 Supersedes EN ISO 11348-1:1998 English Version Water quality Determination of the inhibitory effect of water samples on the
30、light emission of Vibrio fischeri (Luminescent bacteria test) Part 1: Method using freshly prepared bacteria (ISO 11348-1:2007) Qualit de leau Dtermination de leffet inhibiteur dchantillons deau sur la luminescence de Vibrio fischeri (Essai de bactries luminescentes) Partie 1: Mthode utilisant des b
31、actries frachement prpares (ISO 11348-1:2007) Wasserbeschaffenheit Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission von Vibrio fischeri (Leuchtbakterientest) Teil 1: Verfahren mit frisch gezchteten Bakterien (ISO 11348-1:2007) This European Standard was approved by CEN on 29 October
32、 2008. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtaine
33、d on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management
34、 Centre has the same status as theofficial versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Po
35、land, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2008 CEN All rights of exploitation in any form and
36、by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11348-1:2008: EContents Page Foreword. 3 Introduction 4 1 Scope . 5 2 Normative references . 5 3 Principle. 6 4 Interferences . 6 5 Reagents and materials . 6 6 Apparatus 8 7 Sampling and sample pretreatment 9 8 Cultivation of
37、 luminescent bacteria 9 9 Procedure 11 10 Evaluation 12 11 Expression of results . 14 12 Criteria of validity 15 13 Precision 16 14 Test report . 16 Annex A (informative) Colour-correction method. 17 Annex B (informative) Dilution level D Preparation of the dilution series. 20 Annex C (informative)
38、Precision data. 23 Annex D (informative) Testing salt water samples with the luminescent bacteria test with freshly cultured bacteria. 24 Bibliography . 27 2DIN EN ISO 11348-1:2009-05 EN ISO 11348-1:2008 (E) 3DIN EN ISO 11348-1:2009-05 EN ISO 11348-1:2008 (E) Foreword The text of ISO 11348-1:2007 ha
39、s been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 11348-1:2008 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be giv
40、en the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2009, and conflicting national standards shall be withdrawn at the latest by May 2009. Attention is drawn to the possibility that some of the elements of this document may be the
41、subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 11348-1:1998. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to impl
42、ement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland
43、 and the United Kingdom. Endorsement notice The text of ISO 11348-1:2007 has been approved by CEN as a EN ISO 11348-1:2008 without any modification. Introduction The measurements specified in ISO 11348 can be carried out using freshly prepared bacteria, as well as freeze-dried or liquid-dried bacter
44、ial preparations. Standardized work carried out by DIN Normenausschuss Wasserwesen and ISO/TC 147/SC 5/WG 1 has shown that, in special cases, these different techniques may deliver different results, especially in the presence of heavy metals. Such varying sensitivity is caused by differences in med
45、ia composition used in the preparation of freeze-dried or liquid-dried bacteria. These protective media influence the bioavailability of toxicants and/or the light emission of luminescent bacteria. This means that the origin and type of preparation need to be taken into account when interpreting the
46、 results. This may be difficult sometimes, as freeze-dried and liquid-dried bacteria may be obtained from different suppliers. This, in turn, can mean that the composition is not known in detail and therefore cannot be interpreted by the user. For this reason, in addition to toxicity measurements wi
47、th liquid-dried bacteria (ISO 11348-2) and freeze-dried bacteria (ISO 11348-3), a procedure with freshly prepared bacteria is described in this part of ISO 11348, the performance of which can be interpreted by the user in every detail. The laboratories responsible for the results have the opportunit
48、y to select the most suitable technique based on expert judgement and information about the water sample to be tested. 4DIN EN ISO 11348-1:2009-05 EN ISO 11348-1:2008 (E) WARNING Persons using this part of ISO 11348 should be familiar with normal laboratory practice. This standard does not purport t
49、o address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted in accordance with this part of ISO 11348 be carried out by suitably trained staff. 1 Scope ISO 11348 describes three methods for determining the inhibition of the luminescence e
